Propofol Attenuates Odontogenic/osteogenic Differentiation of Human Dental Pulp Stem Cells In vitro

Overview

Journal J Dent Sci

Specialty Dentistry

Date 2022 Oct 27

PMID 36299329

Authors

Eun-Ji Choi

Cheul-Hong Kim

Ji-Young Yoon

Joo-Young Kim

Hyang-Sook Kim

Ji-Uk Yoon

Ah-Reum Cho

Eun-Jung Kim

Affiliations

Soon will be listed here.

Abstract

Background/purpose: Various studies have used stem cells in the field of bone tissue engineering to repair bone defects. Dental pulp stem cells (DPSCs) have multipotent properties and can be acquired in a noninvasive manner; therefore, they are frequently used in experiments in regenerative medicine. The objective of this study was to investigate the odontogenic/osteogenic differentiation of human DPSCs (hDPSCs) using propofol, a widely used intravenous anesthetic agent.

Materials And Methods: Alkaline phosphatase (ALP) staining was used to investigate the effects of various concentrations of propofol (5, 20, 50 and 100 μM) on the osteogenic differentiation of hDPSCs. Real-time qPCR and Western blot analysis were used to detect the effect of propofol on the expression of odontogenic/osteogenic genes, such as DMP1, RUNX2, OCN, and BMP2. Odontogenic/osteogenic differentiation of hDPSCs was estimated at days 7 and 14.

Results: ALP staining of hDPSCs was significantly decreased by propofol treatment. The mRNA expression of DMP1, RUNX2, OCN, and BMP2 decreased after propofol treatment for 14 days. The protein expression of DMP1 and BMP2 was decreased by propofol at days 7 and 14, and that of RUNX2 was decreased by propofol at day 14 only.

Conclusion: Propofol attenuated odontogenic/osteogenic differentiation of hDPSCs in vitro. This result suggests that propofol, which is widely used for dental sedation, may inhibit the odontogenic/osteogenic differentiation of hDPSCs.

References

Chamieh F, Collignon A, Coyac B, Lesieur J, Ribes S, Sadoine J . Accelerated craniofacial bone regeneration through dense collagen gel scaffolds seeded with dental pulp stem cells. Sci Rep. 2016; 6:38814. PMC: 5146967. DOI: 10.1038/srep38814. View

Lee D, Kwon J, Kim H, Lee H, Kim E, Kim H . Propofol attenuates osteoclastogenesis by lowering RANKL/OPG ratio in mouse osteoblasts. Int J Med Sci. 2018; 15(7):723-729. PMC: 6001417. DOI: 10.7150/ijms.22713. View

Short T, Aun C, Tan P, Wong J, Tam Y, Oh T . A prospective evaluation of pharmacokinetic model controlled infusion of propofol in paediatric patients. Br J Anaesth. 1994; 72(3):302-6. DOI: 10.1093/bja/72.3.302. View

Takebe Y, Tatehara S, Fukushima T, Tokuyama-Toda R, Yasuhara R, Mishima K . Cryopreservation Method for the Effective Collection of Dental Pulp Stem Cells. Tissue Eng Part C Methods. 2017; 23(5):251-261. DOI: 10.1089/ten.TEC.2016.0519. View

Bose S, Roy M, Bandyopadhyay A . Recent advances in bone tissue engineering scaffolds. Trends Biotechnol. 2012; 30(10):546-54. PMC: 3448860. DOI: 10.1016/j.tibtech.2012.07.005. View

Li Q, Lu J, Wang X . Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells. Neural Regen Res. 2015; 9(22):2002-7. PMC: 4283284. DOI: 10.4103/1673-5374.145384. View

Lee M, Topper S, Hubler S, Hose J, Wenger C, Coon J . A dynamic model of proteome changes reveals new roles for transcript alteration in yeast. Mol Syst Biol. 2011; 7:514. PMC: 3159980. DOI: 10.1038/msb.2011.48. View

Tamaoki N, Takahashi K, Tanaka T, Ichisaka T, Aoki H, Takeda-Kawaguchi T . Dental pulp cells for induced pluripotent stem cell banking. J Dent Res. 2010; 89(8):773-8. DOI: 10.1177/0022034510366846. View

Zhang W, Walboomers X, van Osch G, van den Dolder J, Jansen J . Hard tissue formation in a porous HA/TCP ceramic scaffold loaded with stromal cells derived from dental pulp and bone marrow. Tissue Eng Part A. 2008; 14(2):285-94. DOI: 10.1089/tea.2007.0146. View

10.

Alge D, Zhou D, Adams L, Wyss B, Shadday M, Woods E . Donor-matched comparison of dental pulp stem cells and bone marrow-derived mesenchymal stem cells in a rat model. J Tissue Eng Regen Med. 2009; 4(1):73-81. PMC: 2830796. DOI: 10.1002/term.220. View

11.

Bai Y, Bai Y, Matsuzaka K, Hashimoto S, Kokubu E, Wang X . Formation of bone-like tissue by dental follicle cells co-cultured with dental papilla cells. Cell Tissue Res. 2010; 342(2):221-31. DOI: 10.1007/s00441-010-1046-9. View

12.

Liang C, Du F, Wang J, Cang J, Xue Z . Propofol Regulates Neural Stem Cell Proliferation and Differentiation via Calmodulin-Dependent Protein Kinase II/AMPK/ATF5 Signaling Axis. Anesth Analg. 2018; 129(2):608-617. DOI: 10.1213/ANE.0000000000003844. View

13.

Tabatabaei F, Torshabi M . In vitro proliferation and osteogenic differentiation of endometrial stem cells and dental pulp stem cells. Cell Tissue Bank. 2017; 18(2):239-247. DOI: 10.1007/s10561-017-9620-y. View

14.

Kichenbrand C, Velot E, Menu P, Moby V . Dental Pulp Stem Cell-Derived Conditioned Medium: An Attractive Alternative for Regenerative Therapy. Tissue Eng Part B Rev. 2018; 25(1):78-88. DOI: 10.1089/ten.TEB.2018.0168. View

15.

Graziano A, DAquino R, Laino G, Papaccio G . Dental pulp stem cells: a promising tool for bone regeneration. Stem Cell Rev. 2008; 4(1):21-6. DOI: 10.1007/s12015-008-9013-5. View

16.

Zheng Y, Liu Y, Zhang C, Zhang H, Li W, Shi S . Stem cells from deciduous tooth repair mandibular defect in swine. J Dent Res. 2009; 88(3):249-54. PMC: 2829885. DOI: 10.1177/0022034509333804. View

17.

Yu J, Wang Y, Deng Z, Tang L, Li Y, Shi J . Odontogenic capability: bone marrow stromal stem cells versus dental pulp stem cells. Biol Cell. 2007; 99(8):465-74. DOI: 10.1042/BC20070013. View

18.

Le Roch K, Johnson J, Florens L, Zhou Y, Santrosyan A, Grainger M . Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle. Genome Res. 2004; 14(11):2308-18. PMC: 525690. DOI: 10.1101/gr.2523904. View

19.

Kim E, Choi I, Yoon J, Park B, Yoon J, Kim C . Effects of propofol-induced autophagy against oxidative stress in human osteoblasts. J Dent Anesth Pain Med. 2017; 16(1):39-47. PMC: 5564117. DOI: 10.17245/jdapm.2016.16.1.39. View

20.

Iohara K, Zheng L, Ito M, Tomokiyo A, Matsushita K, Nakashima M . Side population cells isolated from porcine dental pulp tissue with self-renewal and multipotency for dentinogenesis, chondrogenesis, adipogenesis, and neurogenesis. Stem Cells. 2006; 24(11):2493-503. DOI: 10.1634/stemcells.2006-0161. View