Continuous Oral Administration of Sonicated Delays Rat Skeletal Muscle Healing Post-Treadmill Training

Overview

Journal Int J Environ Res Public Health

Publisher MDPI

Specialties Environmental Health
Public Health

Date 2022 Oct 27

PMID 36293631

Authors

Kairi Hayashi

Yasuo Takeuchi

Shintaro Shimizu

Gen Tanabe

Hiroshi Churei

Hiroaki Kobayashi

Toshiaki Ueno

Affiliations

Soon will be listed here.

Abstract

Background: A delay in muscle repair interferes with the effect of training or exercise; therefore, it is important to identify the factors that delay muscle repair. , one of the most common periodontal disease pathogens, has the potential to inhibit muscle repair after training, as inferred from a previous study. To assess the expression of satellite cells in this in vivo study, we evaluated the relationship between and muscle regeneration after training.

Methods: A total of 20 male Wistar rats (eight weeks in age) were randomly divided into two groups: one orally administered sonicated four times per week for six weeks (PG group) and one given no treatment (NT group). After four weeks of training using a treadmill, the gastrocnemius was evaluated using histology of the cross-sectional area (CSA) of myotubes and immunohistochemistry of the expression of skeletal muscle satellite cells. In addition, an endurance test was performed a day before euthanization.

Results: The CSA and expression of Pax7+/MyoD- and Pax7+/MyoD+ cells were not significantly different between the groups. However, the expression of Pax7-/MyoD+ cells and running time until exhaustion were significantly lower in the PG group.

Conclusions: Infection with likely interferes with muscle repair after training.

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References

Yin H, Price F, Rudnicki M . Satellite cells and the muscle stem cell niche. Physiol Rev. 2013; 93(1):23-67. PMC: 4073943. DOI: 10.1152/physrev.00043.2011. View

Sanz M, Marco Del Castillo A, Jepsen S, Gonzalez-Juanatey J, DAiuto F, Bouchard P . Periodontitis and cardiovascular diseases: Consensus report. J Clin Periodontol. 2020; 47(3):268-288. PMC: 7027895. DOI: 10.1111/jcpe.13189. View

Kumar P . From focal sepsis to periodontal medicine: a century of exploring the role of the oral microbiome in systemic disease. J Physiol. 2016; 595(2):465-476. PMC: 5233655. DOI: 10.1113/JP272427. View

Tang L, Zhang J, Zhao X, Li N, Jian W, Sun S . Low-Intensity Pulsed Ultrasound Promotes Exercise-Induced Muscle Hypertrophy. Ultrasound Med Biol. 2017; 43(7):1411-1420. DOI: 10.1016/j.ultrasmedbio.2017.02.017. View

Carrouel F, Viennot S, Santamaria J, Veber P, Bourgeois D . Quantitative Molecular Detection of 19 Major Pathogens in the Interdental Biofilm of Periodontally Healthy Young Adults. Front Microbiol. 2016; 7:840. PMC: 4889612. DOI: 10.3389/fmicb.2016.00840. View

Kojima T, Yano K, Ishikawa I . Relationship between serum antibody levels and subgingival colonization of Porphyromonas gingivalis in patients with various types of periodontitis. J Periodontol. 1997; 68(7):618-25. DOI: 10.1902/jop.1997.68.7.618. View

Preshaw P, Alba A, Herrera D, Jepsen S, Konstantinidis A, Makrilakis K . Periodontitis and diabetes: a two-way relationship. Diabetologia. 2011; 55(1):21-31. PMC: 3228943. DOI: 10.1007/s00125-011-2342-y. View

Yusuf F, Brand-Saberi B . Myogenesis and muscle regeneration. Histochem Cell Biol. 2012; 138(2):187-99. DOI: 10.1007/s00418-012-0972-x. View

Nakayama M, Ohara N . Molecular mechanisms of -host cell interaction on periodontal diseases. Jpn Dent Sci Rev. 2017; 53(4):134-140. PMC: 5703693. DOI: 10.1016/j.jdsr.2017.06.001. View

10.

McCarthy J, Mula J, Miyazaki M, Erfani R, Garrison K, Farooqui A . Effective fiber hypertrophy in satellite cell-depleted skeletal muscle. Development. 2011; 138(17):3657-66. PMC: 3152923. DOI: 10.1242/dev.068858. View

11.

Arnold L, Henry A, Poron F, Baba-Amer Y, van Rooijen N, Plonquet A . Inflammatory monocytes recruited after skeletal muscle injury switch into antiinflammatory macrophages to support myogenesis. J Exp Med. 2007; 204(5):1057-69. PMC: 2118577. DOI: 10.1084/jem.20070075. View

12.

Bunte K, Kuhn C, Walther C, Peters U, Aarabi G, Smeets R . Clinical significance of ragA, ragB, and PG0982 genes in Porphyromonas gingivalis isolates from periodontitis patients. Eur J Oral Sci. 2021; 129(3):e12776. DOI: 10.1111/eos.12776. View

13.

Howard K, Gonzalez O, Garneau-Tsodikova S . : where do we stand in our battle against this oral pathogen?. RSC Med Chem. 2021; 12(5):666-704. PMC: 8152699. DOI: 10.1039/d0md00424c. View

14.

Zhang Y, Leveille S, Edward J . Wisdom teeth, periodontal disease, and C-reactive protein in US adults. Public Health. 2020; 187:97-102. DOI: 10.1016/j.puhe.2020.07.039. View

15.

Otis J, Niccoli S, Hawdon N, Sarvas J, Frye M, Chicco A . Pro-inflammatory mediation of myoblast proliferation. PLoS One. 2014; 9(3):e92363. PMC: 3960233. DOI: 10.1371/journal.pone.0092363. View

16.

Baeza M, Morales A, Cisterna C, Cavalla F, Jara G, Isamitt Y . Effect of periodontal treatment in patients with periodontitis and diabetes: systematic review and meta-analysis. J Appl Oral Sci. 2020; 28:e20190248. PMC: 6919200. DOI: 10.1590/1678-7757-2019-0248. View

17.

Tian Z, Jiang S, Zhang M, Wang M, Li J, Zhao R . Detection of satellite cells during skeletal muscle wound healing in rats: time-dependent expressions of Pax7 and MyoD in relation to wound age. Int J Legal Med. 2015; 130(1):163-72. DOI: 10.1007/s00414-015-1251-x. View

18.

Oyaizu T, Enomoto M, Yamamoto N, Tsuji K, Horie M, Muneta T . Hyperbaric oxygen reduces inflammation, oxygenates injured muscle, and regenerates skeletal muscle via macrophage and satellite cell activation. Sci Rep. 2018; 8(1):1288. PMC: 5778072. DOI: 10.1038/s41598-018-19670-x. View

19.

Udagawa S, Katagiri S, Maekawa S, Takeuchi Y, Komazaki R, Ohtsu A . Effect of Porphyromonas gingivalis infection in the placenta and umbilical cord in pregnant mice with low birth weight. Acta Odontol Scand. 2018; 76(6):433-441. DOI: 10.1080/00016357.2018.1426876. View

20.

Ohtsu A, Takeuchi Y, Katagiri S, Suda W, Maekawa S, Shiba T . Influence of Porphyromonas gingivalis in gut microbiota of streptozotocin-induced diabetic mice. Oral Dis. 2019; 25(3):868-880. DOI: 10.1111/odi.13044. View