Single-Cell DNA Methylation Analysis of Chicken Lampbrush Chromosomes

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Oct 27

PMID 36293454

Authors

Artem Nurislamov

Timofey Lagunov

Maria Gridina

Alla Krasikova

Veniamin Fishman

Affiliations

Soon will be listed here.

Abstract

DNA methylation is an essential epigenetic regulation mechanism implicated in transcription and replication control, developmental reprogramming, retroelements silencing and other genomic processes. During mammalian development, a specific DNA methylation pattern should be established in germ cells to allow embryonic development. Less is known about germ cell DNA methylation in other species. To close this gap, we performed a single-cell methylome analysis of chicken diplotene oocytes. We comprehensively characterized methylation patterns in these cells, obtained methylation-based chicken genome segmentation and identified oocyte-specific methylated gene promoters. Our data show that despite the formation of specific transcriptionally hyperactive genome architecture in chicken diplotene oocytes, methylation patterns in these cells closely resemble genomic distribution observed in somatic tissues.

Citing Articles

The first chicken oocyte nucleus whole transcriptomic profile defines the spectrum of maternal mRNA and non-coding RNA genes transcribed by the lampbrush chromosomes.

Krasikova A, Kulikova T, Schelkunov M, Makarova N, Fedotova A, Plotnikov V Nucleic Acids Res. 2024; 52(21):12850-12877.

PMID: 39494543 PMC: 11602149. DOI: 10.1093/nar/gkae941.

Research Topics of the Bioinformatics of Gene Regulation.

Orlov Y, Anashkina A, Kumeiko V, Chen M, Kolchanov N Int J Mol Sci. 2023; 24(10).

PMID: 37240120 PMC: 10218204. DOI: 10.3390/ijms24108774.

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