The Chloroplast Genome of the Lichen Photobiont Sp. DW1 and Its Phylogenetic Implications

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2022 Oct 27

PMID 36292725

Authors

Lidan Wang

Shenglu Zhang

Jinjin Fang

Xinjie Jin

Reyim Mamut

Pan Li

Affiliations

Soon will be listed here.

Abstract

Lichens are symbiotic associations of algae and fungi. The genetic mechanism of the symbiosis of lichens and the influence of symbiosis on the size and composition of the genomes of symbiotic algae have always been intriguing scientific questions explored by lichenologists. However, there were limited data on lichen genomes. Therefore, we isolated and purified a lichen symbiotic alga to obtain a single strain ( sp. DW1), and then obtained its chloroplast genome information by next-generation sequencing (NGS). The chloroplast genome is 129,447 bp in length, and the GC content is 35.2%. Repetitive sequences with the length of 30-35 bp account for 1.27% of the total chloroplast genome. The simple sequence repeats are all mononucleotide repeats. Codon usage analysis showed that the genome tended to use codon ending in A/U. By comparing the length of different regions of Trebouxiophyceae genomes, we found that the changes in the length of exons, introns, and intergenic sequences affect the size of genomes. Trebouxiophyceae had an unstable chloroplast genome structure, with IRs repeatedly losing during evolution. Phylogenetic analysis showed that Trebouxiophyceae is paraphyletic, and sp. DW1 is sister to the clade of and .

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