Abundant and Diverse Endophytic Bacteria Associated with Medicinal Plant Arctium Lappa L. and Their Potential for Host Plant Growth Promoting

Overview

Journal Antonie Van Leeuwenhoek

Publisher Springer

Specialty Microbiology

Date 2022 Oct 25

PMID 36284037

Authors

Jia-Qi Liu

Shu-Mei Chen

Chun-mei Zhang

Ming-Jie Xu

Ke Xing

Cheng-Guo Li

Kui Li

Yu-Qin Zhang

Sheng Qin

Affiliations

Soon will be listed here.

Abstract

Arctium lappa L. is one of the medicinal and food homologous plants in China, which is rich in nutrients and medicinal ingredients. The use of plant growth-promoting (PGP) endophytic bacteria is an alternative to reducing chemical fertilizers in agricultural production. The aim of this study was to analyze the diversity of endophytic bacteria in different cultivars of A. lappa L. collected from two geographical locations in China and evaluate PGP traits of the isolates and their potential PGP ability in greenhouse condition. Endophytic bacterial community was investigated by culture-dependent and culture-independent methods. Isolates were screened and investigated for multiple PGP traits, and representative strains were inoculated host seedlings to evaluate the growth promoting effect. A total of 348 endophytic bacteria were obtained and they were distributed into 4 phyla and 30 genera. In addition, high throughput sequencing revealed more abundant bacterial community, including 17 bacterial phyla and 207 genera. A high proportion of PGP traits were detected, including production of indole acetic acid, siderophore, ammonia and phosphate solubilization. Four representative strains with multiple PGP traits of the most dominant genera (Bacillus, Pantoea, Microbacterium and Pseudomonas) were further selected for host inoculation and growth promoting evaluation, and they significantly increase seedlings length, root length and fresh weight. This study demonstrated that A. lappa L. harbors abundant endophytic bacteria, and some endophytic bacteria showed good potential for the development of microbial fertilizer in the future.

Citing Articles

Bioactive Compounds Produced by Endophytic Bacteria and Their Plant Hosts-An Insight into the World of Chosen Herbaceous Ruderal Plants in Central Europe.

Drozdzynski P, Rutkowska N, Rodziewicz M, Marchut-Mikolajczyk O Molecules. 2024; 29(18).

PMID: 39339451 PMC: 11433698. DOI: 10.3390/molecules29184456.

Endophytic Bacterium sp. nov. with Plant Growth-Promoting Function, Isolated from the Seeds of .

Kan Y, Zhang L, Wang Y, Ma Q, Zhou Y, Jiang X Microorganisms. 2023; 11(12).

PMID: 38138043 PMC: 10745605. DOI: 10.3390/microorganisms11122899.

Endophyte-inoculated rhizomes of improve polyphyllin biosynthesis and yield: a transcriptomic analysis of the underlying mechanism.

Zhang Q, Chang S, Yang Y, Xi C, Dong Y, Liu L Front Microbiol. 2023; 14:1261140.

PMID: 38029197 PMC: 10643526. DOI: 10.3389/fmicb.2023.1261140.

Endophytic bacterial communities in ungerminated and germinated seeds of commercial vegetables.

Acuna J, Hu J, Inostroza N, Valenzuela T, Perez P, Epstein S Sci Rep. 2023; 13(1):19829.

PMID: 37963999 PMC: 10645892. DOI: 10.1038/s41598-023-47099-4.

Diversity of Endophytic Microbes in and Their Potential for Plant Growth Promotion and Taxane Accumulation.

Liu Q, Li L, Chen Y, Wang S, Xue L, Meng W Microorganisms. 2023; 11(7).

PMID: 37512818 PMC: 10383522. DOI: 10.3390/microorganisms11071645.

References

Afzal I, Shinwari Z, Sikandar S, Shahzad S . Plant beneficial endophytic bacteria: Mechanisms, diversity, host range and genetic determinants. Microbiol Res. 2019; 221:36-49. DOI: 10.1016/j.micres.2019.02.001. View

Bargaz A, Lyamlouli K, Chtouki M, Zeroual Y, Dhiba D . Soil Microbial Resources for Improving Fertilizers Efficiency in an Integrated Plant Nutrient Management System. Front Microbiol. 2018; 9:1606. PMC: 6079243. DOI: 10.3389/fmicb.2018.01606. View

Biessy A, Filion M . Phenazines in plant-beneficial Pseudomonas spp.: biosynthesis, regulation, function and genomics. Environ Microbiol. 2018; 20(11):3905-3917. DOI: 10.1111/1462-2920.14395. View

Chan Y, Cheng L, Wu J, Chan E, Kwan Y, Lee S . A review of the pharmacological effects of Arctium lappa (burdock). Inflammopharmacology. 2010; 19(5):245-54. DOI: 10.1007/s10787-010-0062-4. View

Chaudhry V, Sharma S, Bansal K, Patil P . Glimpse into the Genomes of Rice Endophytic Bacteria: Diversity and Distribution of Firmicutes. Front Microbiol. 2017; 7:2115. PMC: 5215499. DOI: 10.3389/fmicb.2016.02115. View

Chelius M, Triplett E . The Diversity of Archaea and Bacteria in Association with the Roots of Zea mays L. Microb Ecol. 2001; 41(3):252-263. DOI: 10.1007/s002480000087. View

Chen P, Zhang C, Ju X, Xiong Y, Xing K, Qin S . Community Composition and Metabolic Potential of Endophytic Actinobacteria From Coastal Salt Marsh Plants in Jiangsu, China. Front Microbiol. 2019; 10:1063. PMC: 6527748. DOI: 10.3389/fmicb.2019.01063. View

Chen M, Xu J, Wang Y, Wang Z, Guo L, Li X . Arctium lappa L. polysaccharide can regulate lipid metabolism in type 2 diabetic rats through the SREBP-1/SCD-1 axis. Carbohydr Res. 2020; 494:108055. DOI: 10.1016/j.carres.2020.108055. View

Chernin L, Ismailov Z, Haran S, Chet I . Chitinolytic Enterobacter agglomerans Antagonistic to Fungal Plant Pathogens. Appl Environ Microbiol. 1995; 61(5):1720-6. PMC: 1388435. DOI: 10.1128/aem.61.5.1720-1726.1995. View

10.

Chialva M, Lanfranco L, Bonfante P . The plant microbiota: composition, functions, and engineering. Curr Opin Biotechnol. 2021; 73:135-142. DOI: 10.1016/j.copbio.2021.07.003. View

11.

Coombs J, Franco C . Isolation and identification of actinobacteria from surface-sterilized wheat roots. Appl Environ Microbiol. 2003; 69(9):5603-8. PMC: 194995. DOI: 10.1128/AEM.69.9.5603-5608.2003. View

12.

Sergio Pedroso Costa Junior P, Cardoso F, Martins A, Teixeira Buttros V, Pasqual M, Dias D . Endophytic bacteria of garlic roots promote growth of micropropagated meristems. Microbiol Res. 2020; 241:126585. DOI: 10.1016/j.micres.2020.126585. View

13.

Dobereiner , Evans , Seifert , Wortis . Spinodal fluctuations of budding vesicles. Phys Rev Lett. 1995; 75(18):3360-3363. DOI: 10.1103/PhysRevLett.75.3360. View

14.

El-Sayed W, Akhkha A, El-Naggar M, Elbadry M . In vitro antagonistic activity, plant growth promoting traits and phylogenetic affiliation of rhizobacteria associated with wild plants grown in arid soil. Front Microbiol. 2014; 5:651. PMC: 4255609. DOI: 10.3389/fmicb.2014.00651. View

15.

Etesami H, Maheshwari D . Use of plant growth promoting rhizobacteria (PGPRs) with multiple plant growth promoting traits in stress agriculture: Action mechanisms and future prospects. Ecotoxicol Environ Saf. 2018; 156:225-246. DOI: 10.1016/j.ecoenv.2018.03.013. View

16.

Fadiji A, Ayangbenro A, Babalola O . Metagenomic profiling of the community structure, diversity, and nutrient pathways of bacterial endophytes in maize plant. Antonie Van Leeuwenhoek. 2020; 113(11):1559-1571. DOI: 10.1007/s10482-020-01463-w. View

17.

Fan B, Wang C, Song X, Ding X, Wu L, Wu H . FZB42 in 2018: The Gram-Positive Model Strain for Plant Growth Promotion and Biocontrol. Front Microbiol. 2018; 9:2491. PMC: 6198173. DOI: 10.3389/fmicb.2018.02491. View

18.

Gao Q, Yang M, Zuo Z . Overview of the anti-inflammatory effects, pharmacokinetic properties and clinical efficacies of arctigenin and arctiin from Arctium lappa L. Acta Pharmacol Sin. 2018; 39(5):787-801. PMC: 5943914. DOI: 10.1038/aps.2018.32. View

19.

Gao J, Xue J, Yan H, Tong S, Khan M, Wang L . Pantoea endophytica sp. nov., novel endophytic bacteria isolated from maize planting in different geographic regions of northern China. Syst Appl Microbiol. 2019; 42(4):488-494. DOI: 10.1016/j.syapm.2019.06.001. View

20.

Gong Y, Bai J, Yang H, Zhang W, Xiong Y, Ding P . Phylogenetic diversity and investigation of plant growth-promoting traits of actinobacteria in coastal salt marsh plant rhizospheres from Jiangsu, China. Syst Appl Microbiol. 2018; 41(5):516-527. DOI: 10.1016/j.syapm.2018.06.003. View