Reusable Molecularly Imprinted Electrochemiluminescence Assay for Kanamycin Based on Ordered Mesoporous Carbon Loaded with Indium Oxide Nanoparticles and Carbon Quantum Dots

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2022 Oct 25

PMID 36284001

Authors

Shuhuai Li

Yuwei Wu

Chaohai Pang

Xionghui Ma

Mingyue Wang

Jinhui Luo

Xu Zhi

Bei Li

Affiliations

Soon will be listed here.

Abstract

A highly sensitive kanamycin electrochemiluminescence (ECL) switch sensor was constructed. A signal element consisting of ordered mesoporous carbon loaded with indium oxide nanoparticles/carbon quantum dots (OMC/InO/C-dots) was assembled on the surface of a gold electrode. Then, a molecularly imprinted polymer (MIP) was prepared on the modified electrode surface using kanamycin as the template molecule and o-aminophenol as the functional monomer. After kanamycin elution, the prepared sensor retained specific kanamycin recognition sites. OMC/InO effectively amplified the ECL signal of the C-dots, thereby enhancing the detection sensitivity, whereas kanamycin quenched the signal. Therefore, the imprinted sites acted as a switch, providing a new method for detecting kanamycin. Under the optimal experimental conditions, the concentration of kanamycin was proportional to the degree of ECL quenching within a linear range of 5-4500 × 10 mol L at 0.8 V (vs. Ag/AgCl electrode electrode), and the detection limit was 5.8 × 10 mol L. When applied to the detection of kanamycin in actual samples, such as chicken, duck, pork, and milk, the recovery for spiked samples was in the range 92.7-110%.

References

Sagwa E, Ruswa N, Mavhunga F, Rennie T, Leufkens H, Mantel-Teeuwisse A . Comparing amikacin and kanamycin-induced hearing loss in multidrug-resistant tuberculosis treatment under programmatic conditions in a Namibian retrospective cohort. BMC Pharmacol Toxicol. 2015; 16:36. PMC: 4675035. DOI: 10.1186/s40360-015-0036-7. View

Hamidi H, Zarrineh M, Es-Haghi A, Ghasempour A . Rapid and sensitive determination of neomycin and kanamycin in measles, mumps, and rubella vaccine via high-performance liquid chromatography-tandem mass spectrometry using modified super-paramagnetic FeO nanospheres. J Chromatogr A. 2020; 1625:461343. DOI: 10.1016/j.chroma.2020.461343. View

Yu S, Wei Q, Du B, Wu D, Li H, Yan L . Label-free immunosensor for the detection of kanamycin using Ag@Fe₃O₄ nanoparticles and thionine mixed graphene sheet. Biosens Bioelectron. 2013; 48:224-9. DOI: 10.1016/j.bios.2013.04.025. View

Han S, Li B, Song Z, Pan S, Zhang Z, Yao H . A kanamycin sensor based on an electrosynthesized molecularly imprinted poly-o-phenylenediamine film on a single-walled carbon nanohorn modified glassy carbon electrode. Analyst. 2016; 142(1):218-223. DOI: 10.1039/c6an02338j. View

Lin Y, Wang L, Cheng Y, Lee C, Tsai H . Molecularly Imprinted Polymer/Anodic Aluminum Oxide Nanocomposite Sensing Electrode for Low-Concentration Troponin T Detection for Patient Monitoring Applications. ACS Sens. 2021; 6(6):2429-2435. DOI: 10.1021/acssensors.1c00738. View

Gao X, Gu X, Min Q, Wei Y, Tian C, Zhuang X . Encapsulating Ru(bpy) in an infinite coordination polymer network: Towards a solid-state electrochemiluminescence sensing platform for histamine to evaluate fish product quality. Food Chem. 2021; 368:130852. DOI: 10.1016/j.foodchem.2021.130852. View

Fereja T, Wang C, Liu F, Guan Y, Xu G . A high-efficiency cathodic sodium nitroprusside/luminol/HO electrochemiluminescence system in neutral media for the detection of sodium nitroprusside, glucose, and glucose oxidase. Analyst. 2020; 145(20):6649-6655. DOI: 10.1039/d0an01178a. View

Wang H, Wang Y, Cai L, Liu C, Zhang B, Fang G . Polythionine-mediated AgNWs-AuNPs aggregation conductive network: Fabrication of molecularly imprinted electrochemiluminescence sensors for selective capture of kanamycin. J Hazard Mater. 2022; 434:128882. DOI: 10.1016/j.jhazmat.2022.128882. View

Li S, Li J, Ma X, Pang C, Yin G, Luo J . Molecularly imprinted electroluminescence switch sensor with a dual recognition effect for determination of ultra-trace levels of cobalt (II). Biosens Bioelectron. 2019; 139:111321. DOI: 10.1016/j.bios.2019.111321. View

10.

Li N, Jia L, Ma R, Jia W, Lu Y, Shi S . A novel sandwiched electrochemiluminescence immunosensor for the detection of carcinoembryonic antigen based on carbon quantum dots and signal amplification. Biosens Bioelectron. 2016; 89(Pt 1):453-460. DOI: 10.1016/j.bios.2016.04.020. View

11.

Yang X, Zhao P, Xie Z, Ni M, Wang C, Yang P . Selective determination of epinephrine using electrochemical sensor based on ordered mesoporous carbon / nickel oxide nanocomposite. Talanta. 2021; 233:122545. DOI: 10.1016/j.talanta.2021.122545. View

12.

Hu T, Li T, Yuan L, Liu S, Wang Z . Anodic electrogenerated chemiluminescence of quantum dots: size and stabilizer matter. Nanoscale. 2012; 4(17):5447-53. DOI: 10.1039/c2nr31324c. View

13.

Kaur R, Rana S, Lalit K, Singh P, Kaur K . Electrochemical detection of methyl parathion via a novel biosensor tailored on highly biocompatible electrochemically reduced graphene oxide-chitosan-hemoglobin coatings. Biosens Bioelectron. 2020; 167:112486. DOI: 10.1016/j.bios.2020.112486. View