Investigating SARS-CoV-2 RNA in Five Municipal Wastewater Treatment Plants, Hospital Wastewater and Wastewater Collection Networks During the COVID-19 Pandemic in Ardabil Province, Iran

Overview

Journal Appl Water Sci

Publisher Springer

Date 2022 Oct 24

PMID 36277855

Authors

Abdollah Dargahi

Mehdi Vosoughi

Ali Normohammadi

Anoshirvan Sedigh

Helia Gholizadeh

Hadi Sadeghi

Chiman Karami

Farhad Jeddi

Affiliations

Soon will be listed here.

Abstract

Since 2019, the outbreak of coronavirus with acute respiratory symptoms has caused an epidemic worldwide. Transmission of the disease through respiratory droplets was announced as the main mode of transmission in 2020. But in this study, we discussed the method of indirect transmission of the virus through sewage. In this study, effluents related to urban and hospital wastewater treatment plants in 5 regions of Ardabil Province (northwest of Iran) were investigated. In this research, 120 samples were kept in pre-test conditions (temperature -20 degrees Celsius). To identify the viral genome, special primer and chain reaction probe targeting ORF1ab and N (nucleoprotein gene) genes were used. Out of a total of 120 samples, a total of 3 samples were positive. Wastewater epidemiology (WBE) can be considered as a cost-effective method in the diagnosis and prediction of pathogenic agents. And be considered an effective method for decision-making in order to protect the health of citizens.

Citing Articles

Significance of wastewater surveillance in detecting the prevalence of SARS-CoV-2 variants and other respiratory viruses in the community - A multi-site evaluation.

Khan M, Li L, Haak L, Payen S, Carine M, Adhikari K One Health. 2023; 16:100536.

PMID: 37041760 PMC: 10074727. DOI: 10.1016/j.onehlt.2023.100536.

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