Cytotoxicity of Di-rhamnolipids Produced by RA5 Against Human Cancerous Cell Lines

Overview

Journal 3 Biotech

Publisher Springer

Specialty Biotechnology

Date 2022 Oct 24

PMID 36276467

Authors

Rutuja Ankulkar

Sambhaji Chavan

Durgadevi Aphale

Meera Chavan

Yasmin Mirza

Affiliations

Soon will be listed here.

Abstract

Rhamnolipid biosurfactant produced by , possesses non-toxicity, environmental compatibility, a wide range of pH (4-8), temperature (4-100 °C), and salinity (1-10%) stability. The application of RLs is worldwide accepted in the pharmaceutical, medicinal, and food industries. It has been used for cytotoxicity efficacy analysis with a limited number of cancerous cell lines. To widen the scope of rhamnolipid application as an anticancer agent, we have studied Di-RLs homolog, 'Rha-Rha-C10-C10' produced by RA5 against human cancerous cell lines including breast cancer (MCF-7), leukemia (K-562), cervical cancer (HeLa), Lung cancer (HOP-62), and colon cancer (HT-29) in a dose-dependent way. It was purified with silica gel chromatography followed by TLC and mass spectroscopy prior to cytotoxicity analysis. With a tensiometer, critical micelle concentration of Di-RLs was estimated to be 33.92 ± 2 mN/m at 0.2%. Cytotoxicity analysis of Di-RLs on K-562 cell line demonstrated inhibition with GI50 and TGI at < 10 µg/mL and 66.6 µg/mL, after 48 h of application. The morphology of human cancerous cell lines was observed under a laser confocal microscope with the SRB staining method. Further research is recommended to comprehend the Di-RLs as a potential anti-cancer agent.

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