Expression of Growth Arrest Specific 1 (Gas1) in the Distal Tubules and Collecting Ducts in Normal Kidney and in the Early Stages of Diabetic Nephropathy

Overview

Journal J Mol Histol

Specialty Biochemistry

Date 2022 Oct 22

PMID 36272046

Authors

Brenda I Luna-Antonio

Rafael Rodriguez-Munoz

Carmen Namorado-Tonix

Alejandro Perez-Lopez

Elsa I Sanchez

Paula Vergara

Jose L Reyes

Jose Segovia

Affiliations

Soon will be listed here.

Abstract

The Growth Arrest-Specific protein 1 (Gas1) has been recently described in kidney as an endogenous inhibitor of cell proliferation in mesangial cells and with an important role in the maintenance of nephron progenitor cells. Furthermore, the expression of Gas1 was demonstrated in NCAM + progenitor parietal cells of Bowman's capsule. Thus, the aim of this study was to analyze the expression of Gas1 in the collecting ducts (CD) of healthy rats and to examine whether high glucose levels modify its expression during the early stages of diabetes in STZ-treated rats. Immunofluorescence reveals that principal cells AQP2 + express Gas1 in both healthy and diabetic conditions. Western blot from enriched fractions of medullary CD suggests that diabetes promotes the increase of Gas1. AQP2 + cells are also positive for the expression of CD24 and CD1133 in diabetic rats. In addition, diabetes modifies the cell morphology in the CD and favors the increase of principal cells (AQP2+/Gas1+), induces a significant decrease of intercalated cells (V-ATPase+/Gas1-) and the presence of intermediate cells (Gas1+/V-ATPase+) which express both principal and intercalated cell markers. The expression of Gas1 in the distal tubules was also determined by immunofluorescence, western blot and ELISA in diabetic rats. The results identify Gas1 as a specific marker of principal cells in healthy and diabetic rats and suggest that diabetes promotes the expression of Gas1. Gas1 may have an important role in the maintenance and differentiation to principal cells in the CD during early stages of diabetes.

Citing Articles

Characterization of the pattern of expression of Gas1 in the kidney during postnatal development in the rat.

Cetina-Palma A, Namorado-Tonix C, Rodriguez-Munoz R, Vergara P, Reyes-Sanchez J, Segovia J PLoS One. 2023; 18(4):e0284816.

PMID: 37093844 PMC: 10124827. DOI: 10.1371/journal.pone.0284816.

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