Evaluation of the Antimicrobial Activity of Antiseptics Against Clinical Strains Isolated from Combat Wounds

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2022 Oct 21

PMID 36267170

Authors

Tetyana Valeriyivna Denysko

Oleksandr Adamovych Nazarchuk

Oleksandr Gruzevskyi

Nataliia Anatoliivna Bahniuk

Dmytro Valeriiovych Dmytriiev

Roman Mykolayovych Chornopyschuk

Vira Volodymyrivna Bebyk

Affiliations

Soon will be listed here.

Abstract

Healthcare-associated infections (HCAIs) are among the most prominent medical problems worldwide. In the context of increasing antibiotic resistance globally, the use of antiseptics as the main active agent and potentiator of antibiotics for the treatment of purulent-inflammatory complications of traumatic wounds, burns, and surgical wounds can be considered to tackle opportunistic infections and their prevention during war. This study presents a comparative investigation of the antimicrobial efficacy of antiseptics used for surgical antisepsis and antiseptic treatment of skin, mucous membranes, and wounds against multidrug-resistant clinical isolates of as a wound pathogen of critical priority (according to the WHO). It was found that strains of , which have natural and acquired resistance to antimicrobial drugs, remain susceptible to modern antiseptics. Antiseptic drugs based on decamethoxine, chlorhexidine, octenidine, polyhexanide, and povidone-iodine 10% and 2% provide effective bactericidal activity against within the working concentrations of these drugs. Chlorhexidine and decamethoxine can inhibit biofilm formation by cells. In terms of bactericidal properties and biofilm formation inhibition, chlorhexidine and decamethoxine are the most effective of all tested antiseptics.

References

Junka A, Bartoszewicz M, Smutnicka D, Secewicz A, Szymczyk P . Efficacy of antiseptics containing povidone-iodine, octenidine dihydrochloride and ethacridine lactate against biofilm formed by Pseudomonas aeruginosa and Staphylococcus aureus measured with the novel biofilm-oriented antiseptics test. Int Wound J. 2013; 11(6):730-4. PMC: 7950748. DOI: 10.1111/iwj.12057. View

Loose M, Naber K, Purcell L, Wirth M, Wagenlehner F . Anti-Biofilm Effect of Octenidine and Polyhexanide on Uropathogenic Biofilm-Producing Bacteria. Urol Int. 2021; 105(3-4):278-284. PMC: 8006580. DOI: 10.1159/000512370. View

Kim H, Ryu S, Seo I, Suh S, Suh M, Baek W . Biofilm Formation and Colistin Susceptibility of Acinetobacter baumannii Isolated from Korean Nosocomial Samples. Microb Drug Resist. 2015; 21(4):452-7. DOI: 10.1089/mdr.2014.0236. View

Gunther F, Scherrer M, Kaiser S, DeRosa A, Mutters N . Comparative testing of disinfectant efficacy on planktonic bacteria and bacterial biofilms using a new assay based on kinetic analysis of metabolic activity. J Appl Microbiol. 2016; 122(3):625-633. DOI: 10.1111/jam.13358. View

Jeronimo L, Choi M, Yeon S, Park S, Yoon Y, Choi S . Effects of povidone-iodine composite on the elimination of bacterial biofilm. Int Forum Allergy Rhinol. 2020; 10(7):884-892. DOI: 10.1002/alr.22568. View

Hall C, Mah T . Molecular mechanisms of biofilm-based antibiotic resistance and tolerance in pathogenic bacteria. FEMS Microbiol Rev. 2017; 41(3):276-301. DOI: 10.1093/femsre/fux010. View

Thomas V, Brown R, Ashcraft D, Pankey G . Synergistic effect between nisin and polymyxin B against pandrug-resistant and extensively drug-resistant Acinetobacter baumannii. Int J Antimicrob Agents. 2019; 53(5):663-668. DOI: 10.1016/j.ijantimicag.2019.03.009. View

Huang J, Fan Q, Guo M, Wu M, Wu S, Shen S . Octenidine dihydrochloride treatment of a meticillin-resistant biofilm-infected mouse wound. J Wound Care. 2021; 30(2):106-114. DOI: 10.12968/jowc.2021.30.2.106. View

Krasowski G, Junka A, Paleczny J, Czajkowska J, Makomaska-Szaroszyk E, Chodaczek G . In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens. Membranes (Basel). 2021; 11(1). PMC: 7830887. DOI: 10.3390/membranes11010062. View

10.

Gunther F, Kaiser S, Fries T, Frank U, Mutters N . Susceptibility of multidrug resistant clinical pathogens to a chlorhexidine formulation. J Prev Med Hyg. 2016; 56(4):E176-9. PMC: 4753819. View

11.

Kempf M, Rolain J . Emergence of resistance to carbapenems in Acinetobacter baumannii in Europe: clinical impact and therapeutic options. Int J Antimicrob Agents. 2011; 39(2):105-14. DOI: 10.1016/j.ijantimicag.2011.10.004. View

12.

Leshem T, Gilron S, Azrad M, Peretz A . Characterization of reduced susceptibility to chlorhexidine among Gram-negative bacteria. Microbes Infect. 2021; 24(2):104891. DOI: 10.1016/j.micinf.2021.104891. View

13.

Lanjri S, Uwingabiye J, Frikh M, Abdellatifi L, Kasouati J, Maleb A . In vitro evaluation of the susceptibility of isolates to antiseptics and disinfectants: comparison between clinical and environmental isolates. Antimicrob Resist Infect Control. 2017; 6:36. PMC: 5387265. DOI: 10.1186/s13756-017-0195-y. View

14.

Guo H, Xiang J . [Influences of abaR gene on biofilm formation of ]. Zhonghua Shao Shang Za Zhi. 2017; 33(4):200-205. DOI: 10.3760/cma.j.issn.1009-2587.2017.04.003. View

15.

Christensen G, Simpson W, Younger J, Baddour L, Barrett F, Melton D . Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol. 1985; 22(6):996-1006. PMC: 271866. DOI: 10.1128/jcm.22.6.996-1006.1985. View

16.

Park J, Kim M, Shin B, Kang M, Yang J, Lee T . A novel decoy strategy for polymyxin resistance in . Elife. 2021; 10. PMC: 8324293. DOI: 10.7554/eLife.66988. View

17.

Shahid F, Zaheer T, Ashraf S, Shehroz M, Anwer F, Naz A . Chimeric vaccine designs against Acinetobacter baumannii using pan genome and reverse vaccinology approaches. Sci Rep. 2021; 11(1):13213. PMC: 8225639. DOI: 10.1038/s41598-021-92501-8. View

18.

Davis S, Harding A, Gil J, Parajon F, Valdes J, Solis M . Effectiveness of a polyhexanide irrigation solution on methicillin-resistant Staphylococcus aureus biofilms in a porcine wound model. Int Wound J. 2017; 14(6):937-944. PMC: 7950165. DOI: 10.1111/iwj.12734. View

19.

Shepherd M, Moore G, Wand M, Sutton J, Bock L . Pseudomonas aeruginosa adapts to octenidine in the laboratory and a simulated clinical setting, leading to increased tolerance to chlorhexidine and other biocides. J Hosp Infect. 2018; 100(3):e23-e29. DOI: 10.1016/j.jhin.2018.03.037. View

20.

Pimentel C, Le C, Tuttobene M, Subils T, Martinez J, Sieira R . Human Pleural Fluid and Human Serum Albumin Modulate the Behavior of a Hypervirulent and Multidrug-Resistant (MDR) Representative Strain. Pathogens. 2021; 10(4). PMC: 8069197. DOI: 10.3390/pathogens10040471. View