Molecular Characterization of Six Heat Shock Protein 70 Genes from Arma Chinensis and Their Expression Patterns in Response to Temperature Stress

Overview

Journal Cell Stress Chaperones

Publisher Elsevier

Specialty Cell Biology

Date 2022 Oct 20

PMID 36264419

Authors

Jian-Yu Meng

Chang-Li Yang

Han-Cheng Wang

Yi Cao

Chang-Yu Zhang

Affiliations

Soon will be listed here.

Abstract

Arma chinensis is an important predatory enemy of many agricultural and forest pests. Heat shock protein 70 (Hsp70) plays an essential role in insect adaptation to various stress factors. To explore the functions of Hsp70s in relation to thermal tolerance of A. chinensis, full-length cDNAs of six Hsp70 genes (AcHsp70Ba, AcHsp70-4, AcHsp68a, AcHsp68b, AcHsp70-2, and AcHsc70-4) were cloned. Their open reading frames (ORFs) were 1902, 2454, 1884, 1905, 1872, and 1947 bp, respectively. Developmental expression profiles showed that AcHsp70Ba, AcHsp70-4, and AcHsc70-4 were extremely highly expressed in adult stages. AcHsp68a and AcHsp70-2 showed the highest level of expression in nymph stages, and AcHsp68b was mainly expressed in male adults. Tissue distribution analysis demonstrated that the AcHsp70s were ubiquitously expressed but showing gene-specific and sex-driven patterns of expression. High temperature induced the expression of the six AcHsp70s. Among them, AcHsp70Ba, AcHsp70-4, AcHsp68a, and AcHsc70-4 were significantly induced at 38 °C for 6 h, while all six AcHsp70s were significantly induced at 38 °C for 24 h. There were differences in responses of the six AcHsp70s to low-temperature stress. The expressions of AcHsp70-4, AcHsp68a, and AcHsp68b in male adults were significantly repressed at 4 °C for 6 h, whereas AcHsp70Ba and AcHsp70-2 were significantly induced. The levels of AcHsp70Ba, AcHsp68b, and AcHsp70-2 in female adults were significantly repressed at 4 °C for 24 h, whereas AcHsc70-4 was significantly induced. These results suggested that AcHsp70s play important roles in various developmental stages and tissue function, and contribute to the tolerance of A. chinensis to extreme temperatures.

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References

Kim K, Kim R, Kim S . Crystal structure of a small heat-shock protein. Nature. 1998; 394(6693):595-9. DOI: 10.1038/29106. View

Gao P, Lu M, Pan D, Du Y . Characterization of an inducible HSP70 gene in Chilo suppressalis and expression in response to environmental and biological stress. Cell Stress Chaperones. 2019; 25(1):65-72. PMC: 6985400. DOI: 10.1007/s12192-019-01047-2. View

Choi B, Hepat R, Kim Y . RNA interference of a heat shock protein, Hsp70, loses its protection role in indirect chilling injury to the beet armyworm, Spodoptera exigua. Comp Biochem Physiol A Mol Integr Physiol. 2013; 168:90-5. DOI: 10.1016/j.cbpa.2013.11.011. View

Huang L, Wang C, Kang L . Cloning and expression of five heat shock protein genes in relation to cold hardening and development in the leafminer, Liriomyza sativa. J Insect Physiol. 2009; 55(3):279-85. DOI: 10.1016/j.jinsphys.2008.12.004. View

Xu Q, Zou Q, Zheng H, Zhang F, Tang B, Wang S . Three heat shock proteins from Spodoptera exigua: Gene cloning, characterization and comparative stress response during heat and cold shocks. Comp Biochem Physiol B Biochem Mol Biol. 2011; 159(2):92-102. DOI: 10.1016/j.cbpb.2011.02.005. View

Xu Q, Qin Y . Molecular cloning of heat shock protein 60 (PtHSP60) from Portunus trituberculatus and its expression response to salinity stress. Cell Stress Chaperones. 2012; 17(5):589-601. PMC: 3535163. DOI: 10.1007/s12192-012-0334-6. View

Zhou C, Yang X, Yang H, Long G, Wang Z, Jin D . Effects of abiotic stress on the expression of Hsp70 genes in Sogatella furcifera (Horváth). Cell Stress Chaperones. 2019; 25(1):119-131. PMC: 6985323. DOI: 10.1007/s12192-019-01053-4. View

Shu Y, Du Y, Wang J . Molecular characterization and expression patterns of Spodoptera litura heat shock protein 70/90, and their response to zinc stress. Comp Biochem Physiol A Mol Integr Physiol. 2010; 158(1):102-10. DOI: 10.1016/j.cbpa.2010.09.006. View

Fujikake N, Nagai Y, Popiel H, Kano H, Yamaguchi M, Toda T . Alternative splicing regulates the transcriptional activity of Drosophila heat shock transcription factor in response to heat/cold stress. FEBS Lett. 2005; 579(17):3842-8. DOI: 10.1016/j.febslet.2005.05.074. View

10.

Aamodt R . The caste- and age-specific expression signature of honeybee heat shock genes shows an alternative splicing-dependent regulation of Hsp90. Mech Ageing Dev. 2008; 129(11):632-7. DOI: 10.1016/j.mad.2008.07.002. View

11.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

12.

Chen X, Zhang Y . Identification of multiple small heat-shock protein genes in Plutella xylostella (L.) and their expression profiles in response to abiotic stresses. Cell Stress Chaperones. 2014; 20(1):23-35. PMC: 4255244. DOI: 10.1007/s12192-014-0522-7. View

13.

Zhang C, Dai L, Wang L, Qian C, Wei G, Li J . Inhibitors of eicosanoid biosynthesis influencing the transcripts level of sHSP21.4 gene induced by pathogen infections, in Antheraea pernyi. PLoS One. 2015; 10(4):e0121296. PMC: 4386827. DOI: 10.1371/journal.pone.0121296. View

14.

Sun M, Lu M, Tang X, Du Y . Characterization and expression of genes encoding three small heat shock proteins in Sesamia inferens (Lepidoptera: Noctuidae). Int J Mol Sci. 2014; 15(12):23196-211. PMC: 4284760. DOI: 10.3390/ijms151223196. View

15.

Zhang Q, Denlinger D . Molecular characterization of heat shock protein 90, 70 and 70 cognate cDNAs and their expression patterns during thermal stress and pupal diapause in the corn earworm. J Insect Physiol. 2009; 56(2):138-50. DOI: 10.1016/j.jinsphys.2009.09.013. View

16.

Abarca M, Spahn R . Direct and indirect effects of altered temperature regimes and phenological mismatches on insect populations. Curr Opin Insect Sci. 2021; 47:67-74. DOI: 10.1016/j.cois.2021.04.008. View

17.

Sonoda S, Ashfaq M, Tsumuki H . Cloning and nucleotide sequencing of three heat shock protein genes (hsp90, hsc70, and hsp19.5) from the diamondback moth, Plutella xylostella (L.) and their expression in relation to developmental stage and temperature. Arch Insect Biochem Physiol. 2006; 62(2):80-90. DOI: 10.1002/arch.20124. View

18.

Zhao Q, Wei J, Bu W, Liu G, Zhang H . Synonymize Arma chinensis as Arma custos based on morphological, molecular and geographical data. Zootaxa. 2018; 4455(1):161-176. DOI: 10.11646/zootaxa.4455.1.7. View

19.

Dumas P, Morin M, Boquel S, Moffat C, Morin P . Expression status of heat shock proteins in response to cold, heat, or insecticide exposure in the Colorado potato beetle Leptinotarsa decemlineata. Cell Stress Chaperones. 2019; 24(3):539-547. PMC: 6527667. DOI: 10.1007/s12192-019-00983-3. View

20.

Lu K, Chen X, Liu W, Zhang Z, Wang Y, You K . Characterization of heat shock protein 70 transcript from Nilaparvata lugens (Stål): Its response to temperature and insecticide stresses. Pestic Biochem Physiol. 2017; 142:102-110. DOI: 10.1016/j.pestbp.2017.01.011. View