Transcriptome Sequencing Reveals the Difference in the Expression of Biofilm and Planktonic Cells Between Two Strains of Typhimurium

Overview

Journal Biofilm

Date 2022 Oct 18

PMID 36254114

Authors

Liping Zheng

Xinyi Zhang

Zhaoxin Lu

Wenjie Ma

Antuo Hu

Haibo Zhou

Xiaomei Bie

Affiliations

Soon will be listed here.

Abstract

serovar Typhimurium ( Typhimurium) is a food-borne pathogen that can form biofilms to increase its resistance to the external environment. Through the detection of biofilm of several Typhimurium strains in this study, strain CDC3 with strong biofilm forming capacity and strain CVCC3384 with weak biofilm forming capacity were identified. The genes expressed in planktonic and biofilm cells of two . Typhimurium strains were analysed by transcriptome sequencing. Results showed that the genes related to the signal transduction pathway were upregulated and genes related to motility were downregulated in strain CDC3. By comparing biofilms and planktonic cells of the two strains, we found that CDC3 regulates biofilm formation mainly through the two-component system , while strain CVCC3384 does so mainly through motility and quorum sensing. This study revealed regulation mechanism of biofilms formation between different biofilm forming capacity strains, and provided a theoretical basis for subsequent research.

Citing Articles

Unraveling the molecular dynamics of biofilms at the air-liquid interface.

Sung K, Park M, Chon J, Kweon O, Khan S Future Microbiol. 2024; 19(8):681-696.

PMID: 38661712 PMC: 11259063. DOI: 10.2217/fmb-2023-0234.

Molecular Aspects of the Functioning of Pathogenic Bacteria Biofilm Based on (QS) Signal-Response System and Innovative Non-Antibiotic Strategies for Their Elimination.

Juszczuk-Kubiak E Int J Mol Sci. 2024; 25(5).

PMID: 38473900 PMC: 10931677. DOI: 10.3390/ijms25052655.

How to study biofilms: technological advancements in clinical biofilm research.

Cleaver L, Garnett J Front Cell Infect Microbiol. 2023; 13:1335389.

PMID: 38156318 PMC: 10753778. DOI: 10.3389/fcimb.2023.1335389.

What's in a name? Characteristics of clinical biofilms.

Lichtenberg M, Coenye T, Parsek M, Bjarnsholt T, Jakobsen T FEMS Microbiol Rev. 2023; 47(5).

PMID: 37656883 PMC: 10503651. DOI: 10.1093/femsre/fuad050.

References

Kalai Chelvam K, Chai L, Thong K . Variations in motility and biofilm formation of Salmonella enterica serovar Typhi. Gut Pathog. 2014; 6(1):2. PMC: 3922113. DOI: 10.1186/1757-4749-6-2. View

Eran Z, Akcelik M, Yazici B, Ozcengiz G, Akcelik N . Regulation of biofilm formation by marT in Salmonella Typhimurium. Mol Biol Rep. 2020; 47(7):5041-5050. DOI: 10.1007/s11033-020-05573-6. View

Barnhart M, Chapman M . Curli biogenesis and function. Annu Rev Microbiol. 2006; 60:131-47. PMC: 2838481. DOI: 10.1146/annurev.micro.60.080805.142106. View

Love M, Huber W, Anders S . Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014; 15(12):550. PMC: 4302049. DOI: 10.1186/s13059-014-0550-8. View

Scallan E, Mahon B, Hoekstra R, Griffin P . Estimates of illnesses, hospitalizations and deaths caused by major bacterial enteric pathogens in young children in the United States. Pediatr Infect Dis J. 2012; 32(3):217-21. DOI: 10.1097/INF.0b013e31827ca763. View

Bertrand J, Altendorf K, Bramkamp M . Amino acid substitutions in putative selectivity filter regions III and IV in KdpA alter ion selectivity of the KdpFABC complex from Escherichia coli. J Bacteriol. 2004; 186(16):5519-22. PMC: 490938. DOI: 10.1128/JB.186.16.5519-5522.2004. View

Eichelberg K, Galan J . The flagellar sigma factor FliA (sigma(28)) regulates the expression of Salmonella genes associated with the centisome 63 type III secretion system. Infect Immun. 2000; 68(5):2735-43. PMC: 97482. DOI: 10.1128/IAI.68.5.2735-2743.2000. View

Chin K, Taylor T, Hebrard M, Anbalagan K, Dashti M, Phua K . Transcriptomic study of Salmonella enterica subspecies enterica serovar Typhi biofilm. BMC Genomics. 2017; 18(1):836. PMC: 5664820. DOI: 10.1186/s12864-017-4212-6. View

Diez-Garcia M, Capita R, Alonso-Calleja C . Influence of serotype on the growth kinetics and the ability to form biofilms of Salmonella isolates from poultry. Food Microbiol. 2012; 31(2):173-80. DOI: 10.1016/j.fm.2012.03.012. View

10.

Saini S, Brown J, Aldridge P, Rao C . FliZ Is a posttranslational activator of FlhD4C2-dependent flagellar gene expression. J Bacteriol. 2008; 190(14):4979-88. PMC: 2447003. DOI: 10.1128/JB.01996-07. View

11.

Borges K, Furian T, de Souza S, Menezes R, Lima D, Fortes F . Biofilm formation by Salmonella Enteritidis and Salmonella Typhimurium isolated from avian sources is partially related with their in vivo pathogenicity. Microb Pathog. 2018; 118:238-241. DOI: 10.1016/j.micpath.2018.03.039. View

12.

El Hag M, Feng Z, Su Y, Wang X, Yassin A, Chen S . Contribution of the csgA and bcsA genes to Salmonella enterica serovar Pullorum biofilm formation and virulence. Avian Pathol. 2017; 46(5):541-547. DOI: 10.1080/03079457.2017.1324198. View

13.

Park S, Jarquin R, Hanning I, Almeida G, Ricke S . Detection of Salmonella spp. survival and virulence in poultry feed by targeting the hilA gene. J Appl Microbiol. 2011; 111(2):426-32. DOI: 10.1111/j.1365-2672.2011.05054.x. View

14.

Jia K, Wang G, Liang L, Wang M, Wang H, Xu X . Preliminary Transcriptome Analysis of Mature Biofilm and Planktonic Cells of Enteritidis Exposure to Acid Stress. Front Microbiol. 2017; 8:1861. PMC: 5622974. DOI: 10.3389/fmicb.2017.01861. View

15.

De Oliveira D, Junior A, Kaneno R, Silva M, Araujo Junior J, Silva N . Ability of Salmonella spp. to produce biofilm is dependent on temperature and surface material. Foodborne Pathog Dis. 2014; 11(6):478-83. DOI: 10.1089/fpd.2013.1710. View

16.

Hamilton S, Bongaerts R, Mulholland F, Cochrane B, Porter J, Lucchini S . The transcriptional programme of Salmonella enterica serovar Typhimurium reveals a key role for tryptophan metabolism in biofilms. BMC Genomics. 2009; 10:599. PMC: 2805695. DOI: 10.1186/1471-2164-10-599. View

17.

Vu B, Chen M, Crawford R, Ivanova E . Bacterial extracellular polysaccharides involved in biofilm formation. Molecules. 2009; 14(7):2535-54. PMC: 6254922. DOI: 10.3390/molecules14072535. View

18.

Claret L, Miquel S, Vieille N, Ryjenkov D, Gomelsky M, Darfeuille-Michaud A . The flagellar sigma factor FliA regulates adhesion and invasion of Crohn disease-associated Escherichia coli via a cyclic dimeric GMP-dependent pathway. J Biol Chem. 2007; 282(46):33275-33283. DOI: 10.1074/jbc.M702800200. View

19.

Du B, Gu Y, Chen G, Wang G, Liu L . Flagellar motility mediates early-stage biofilm formation in oligotrophic aquatic environment. Ecotoxicol Environ Saf. 2020; 194:110340. DOI: 10.1016/j.ecoenv.2020.110340. View

20.

Liu X, Wang C, Yan B, Lyu L, Takiff H, Gao Q . The potassium transporter KdpA affects persister formation by regulating ATP levels in . Emerg Microbes Infect. 2020; 9(1):129-139. PMC: 6968386. DOI: 10.1080/22221751.2019.1710090. View