Mobile ARGONAUTE 1d Binds 22-nt MiRNAs to Generate PhasiRNAs Important for Low-temperature Male Fertility in Rice

Overview

Journal Sci China Life Sci

Specialties Biology
Science

Date 2022 Oct 14

PMID 36239908

Authors

Fuyan Si

Haofei Luo

Chao Yang

Jie Gong

Bin Yan

Chunyan Liu

Xianwei Song

Xiaofeng Cao

Affiliations

Soon will be listed here.

Abstract

Phased small interfering RNAs (phasiRNAs) are abundantly expressed in anthers and linked to environment-related male fertility in grasses, yet how they function under different environmental conditions remains unclear. Here, we identified a rice (Oryza sativa) low temperature-induced Argonaute (AGO) protein, OsAGO1d, that is responsible for generating phasiRNAs and preserving male fertility at low temperature. Loss of OsAGO1d function causes low-temperature male sterility associated with delayed programmed cell death of tapetal cells during anther development. OsAGO1d binds miR2118 and miR2275 family members and triggers phasiRNA biogenesis; it also binds 21-nt phasiRNAs with a 5' terminal U. In total, phasiRNAs from 972 loci are OsAGO1d-dependent. OsAGO1d protein moves from anther wall cells into meiocytes, where it loads miR2275 to produce 24-nt phasiRNAs. Together, our results show that OsAGO1d acts as a mobile signal to fine-tune phasiRNA production and this function is important for male fertility at low temperature.

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