Noncoding RNAs Responsive to Nitric Oxide and Their Protein-coding Gene Targets Shed Light on Root Hair Formation in

Overview

Journal Front Genet

Date 2022 Oct 14

PMID 36238154

Authors

Camilla Alves Santos

Camila Fernandes Moro

Ione Salgado

Marcia Regina Braga

Marilia Gaspar

Affiliations

Soon will be listed here.

Abstract

An overview of the total transcriptome, described previously by our research group, pointed some noncoding RNA (ncRNA) as participants in the restoration of hair-root phenotype in mutants, leading us to a deeper investigation. A transcriptional gene expression profiling of seedling roots was performed aiming to identify ncRNA responsive to nitric oxide (GSNO) and auxin (IAA), and their involvement in root hair formation in the null mutant. We identified 3,631 ncRNAs, including new ones, in and differential expression (DE) analysis between the following: 1) GSNO-treated vs. untreated , 2) IAA-treated vs. untreated , 3) GSNO-treated vs. IAA-treated , and 4) WS-2 vs. untreated detected the greatest number of DE genes in GSNO-treated . We detected hundreds of interactions among ncRNA and protein-coding genes (PCGs), highlighting MIR5658 and MIR171 precursors highly upregulated in GSNO-treated and wild type, respectively. Those ncRNA interact with many DE PCGs involved in hormone signaling, cell wall development, transcription factors, and root hair formation, becoming candidate genes in cell wall modulation and restoration of root hair phenotype by GSNO treatment. Our data shed light on how GSNO modulates ncRNA and their PCG targets in root hair formation.

Citing Articles

Role of microRNA miR171 in plant development.

Pei L, Zhang L, Liu X, Jiang J PeerJ. 2023; 11:e15632.

PMID: 37456878 PMC: 10340099. DOI: 10.7717/peerj.15632.

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