Intensification of Polyphenols Extraction from Leaves Using and Evaluation of Antibiofilm and Antibacterial Activities

Overview

Journal Plants (Basel)

Date 2022 Oct 14

PMID 36235324

Authors

Mariam Hammoud

Ali Chokr

Hiba N Rajha

Carl Safi

Martijn van Walsem

Lambertus A M van den Broek

Esperance Debs

Richard G Maroun

Nicolas Louka

Hassan Rammal

Affiliations

Soon will be listed here.

Abstract

(1) Background: is a plant medicinally valued, and used in pharmacopeia to treat various diseases. No previous studies have been reported on leaf extracts using an IR-assisted technique; thus, this study aimed to intensify polyphenol extraction using , comparing it to the conventional water bath (WB) method. (2) Methods: Optimization of polyphenol extraction from leaves was conducted using Response Surface Methodology. was used and compared to the WB method. The biological activities (antiradical, antioxidant, antibacterial, and antibiofilm) of both extracts were assessed. UHPLC analysis was performed to analyze the phytochemical profile of both extracts. (3) Results: Under optimal conditions, IR improved the polyphenol extraction yield by 1.7 times, while lowering ethanol consumption by 1.5 times. Regarding the antibacterial activity, both WB and IR leaf extracts exhibited the highest antibacterial activity against . The maximum biofilm prevention capacity was also noticed against . UHPLC-MS analysis quantified two major phenolic compounds in both extracts: rutin and sinapic acid. (4) Conclusions: technology proved to be an effective technique in intensifying polyphenol recovery, while preserving their quantity and quality.

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