Induces Cell Death in Cervical Cancer Squamous Epithelial Cells Via Unfolded Protein Accumulation and Endoplasmic Reticulum Stress

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Oct 14

PMID 36235027

Authors

Alejandro Montoya-Gomez

Nelson Rivera Franco

Leonel Ives Montealegre-Sanchez

Luis Manuel Solano-Redondo

Andres Castillo

Mildrey Mosquera-Escudero

Eliecer Jimenez-Charris

Affiliations

Soon will be listed here.

Abstract

Due to the lack of chemotherapeutic drugs that selectively affect cervical cancer cells, natural sources such as snake venom are currently being investigated for molecules with antitumor potential. , a phospholipase A type-Asp49 from snake venom, induced cell death in a cervical cancer cell line-Ca Ski-related to dysfunction in the ability to resolve endoplasmic reticulum stress, evidenced by sub-expression of genes such as PERK, ERO1 PDIs, HSP70, and CHOP. Western blot analysis validated the last two genes' sub-expression at the protein level. In addition, presented a dose-dependent cytotoxic effect on cancer cells and an insignificant effect on healthy endothelial cells (HUVEC). Additionally, inhibited cancer cells' adhesion and migration capacity, induced cell cycle arrest in the G2/M phase, and induced apoptosis stimulated possibly by the extrinsic route. These results demonstrate for the first time that has an antitumor effect on a squamous epithelial cervical cancer cell line and represents a possible biotechnological tool for designing a prominent antitumor agent.

Citing Articles

Snake venom bioprospecting as an approach to finding potential anti-glioblastoma molecules.

Orozco-Mera J, Montoya-Gomez A, Lopes D, Jimenez-Charris E J Venom Anim Toxins Incl Trop Dis. 2024; 30:e20240015.

PMID: 39285908 PMC: 11404105. DOI: 10.1590/1678-9199-JVATITD-2024-0015.

Exploring the Safety of and Antitumoral Potential of Its Recombinant Isoform in Cervical Cancer Therapy.

Sevilla-Sanchez M, Montoya-Gomez A, Osorno-Valencia D, Montealegre-Sanchez L, Mosquera-Escudero M, Jimenez-Charris E Cells. 2023; 12(24).

PMID: 38132131 PMC: 10741390. DOI: 10.3390/cells12242812.

: Unveiling the Action Mechanism of a Promising Chemotherapeutic Agent Targeting Cervical Cancer Cell Adhesion and Survival Pathways.

Montoya-Gomez A, Tonello F, Spolaore B, Massimino M, Montealegre-Sanchez L, Castillo A Cells. 2023; 12(23).

PMID: 38067143 PMC: 10705806. DOI: 10.3390/cells12232715.

Knowledge about Snake Venoms and Toxins from Colombia: A Systematic Review.

Pereanez J, Preciado L, Rey-Suarez P Toxins (Basel). 2023; 15(11).

PMID: 37999521 PMC: 10675826. DOI: 10.3390/toxins15110658.

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