A New Method for Abrin Detection Based on the Interaction Between Target Molecules and Fluorescently Labeled Aptamers on Magnetic Microspheres

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2022 Oct 14

PMID 36234322

Authors

Zhiwei Liu

Zhaoyang Tong

Yuting Wu

Bing Liu

Shasha Feng

Xihui Mu

Jiang Wang

Bin Du

Jianjie Xu

Shuai Liu

Affiliations

Soon will be listed here.

Abstract

A quantitative structure-activity relationship (QSAR) model for the structure and affinity of abrin aptamers was established. A higher affinity abrin aptamer based on the established QSAR model was screened by site-directed mutagenesis. The fluorescence quenching effect between magnetic microspheres and fluorescent molecules was studied for the first time. A new method for abrin detection based on the interaction between target molecules and fluorescently labeled aptamers on magnetic microspheres was developed, with the detection limit of 5 ng mL. This method can overcome the influence of complex environmental interferents in abrin detection and can meet the analysis requirements for simulated samples such as water, soil, and food.

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