Gold-Nanoparticle-Coated Magnetic Beads for ALP-Enzyme-Based Electrochemical Immunosensing in Human Plasma

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2022 Oct 14

PMID 36234217

Authors

Seo-Eun Lee

Se-Eun Jeong

Jae-Sang Hong

Hyungsoon Im

Sei-Young Hwang

Jun Kyun Oh

Seong-Eun Kim

Affiliations

Soon will be listed here.

Abstract

A simple and sensitive AuNP-coated magnetic beads (AMB)-based electrochemical biosensor platform was fabricated for bioassay. In this study, AuNP-conjugated magnetic particles were successfully prepared using biotin-streptavidin conjugation. The morphology and structure of the nanocomplex were characterized by scanning electron microscopy (SEM) with energy-dispersive X-ray analysis (EDX) and UV-visible spectroscopy. Moreover, cyclic voltammetry (CV) was used to investigate the effect of AuNP-MB on alkaline phosphatase (ALP) for electrochemical signal enhancement. An ALP-based electrochemical (EC) immunoassay was performed on the developed AuNP-MB complex with indium tin oxide (ITO) electrodes. Subsequently, the concentration of capture antibodies was well-optimized on the AMB complex via biotin-avidin conjugation. Lastly, the developed AuNP-MB immunoassay platform was verified with extracellular vesicle (EV) detection via immune response by showing the existence of EGFR proteins on glioblastoma multiforme (GBM)-derived EVs (10 particle/mL) spiked in human plasma. Therefore, the signal-enhanced ALP-based EC biosensor on AuNP-MB was favorably utilized as an immunoassay platform, revealing the potential application of biosensors in immunoassays in biological environments.

Citing Articles

Progress in Electrochemical Immunosensors with Alkaline Phosphatase as the Signal Label.

Chen C, La M, Yi X, Huang M, Xia N, Zhou Y Biosensors (Basel). 2023; 13(9).

PMID: 37754089 PMC: 10526794. DOI: 10.3390/bios13090855.

The Applications of Electrochemical Immunosensors in the Detection of Disease Biomarkers: A Review.

Chen H, Zhang J, Huang R, Wang D, Deng D, Zhang Q Molecules. 2023; 28(8).

PMID: 37110837 PMC: 10144570. DOI: 10.3390/molecules28083605.

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