Chlorpyrifos Occurrence and Toxicological Risk Assessment: A Review

Overview

Journal Int J Environ Res Public Health

Publisher MDPI

Specialties Environmental Health
Public Health

Date 2022 Oct 14

PMID 36231509

Authors

Elzbieta Wolejko

Bozena Lozowicka

Agata Jablonska-Trypuc

Marta Pietruszynska

Urszula Wydro

Affiliations

Soon will be listed here.

Abstract

Chlorpyrifos (CPF) was the most frequently used pesticide in food production in the European Union (EU) until 2020. Unfortunately, this compound is still being applied in other parts of the world. National monitoring of pesticides conducted in various countries indicates the presence of CPF in soil, food, and water, which may have toxic effects on consumers, farmers, and animal health. In addition, CPF may influence changes in the population of fungi, bacteria, and actinomycete in soil and can inhibit nitrogen mineralization. The mechanisms of CPF activity are based on the inhibition of acetylcholinesterase (AChE) activity. This compound also exhibits reproductive toxicity, neurotoxicity, and genotoxicity. The problem seems to be the discrepancy between the actual observations and the final conclusions drawn for the substance's approval in reports presenting the toxic impact of CPF on human health. Therefore, this influence is still a current and important issue that requires continuous monitoring despite its withdrawal from the market in the EU. This review traces the scientific reports describing the effects of CPF resulting in changes occurring in both the environment and at the cellular and tissue level in humans and animals. It also provides an insight into the hazards and risks to human health in food consumer products in which CPF has been detected.

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References

Eaton D, Daroff R, Autrup H, Bridges J, Buffler P, Costa L . Review of the toxicology of chlorpyrifos with an emphasis on human exposure and neurodevelopment. Crit Rev Toxicol. 2008; 38 Suppl 2:1-125. DOI: 10.1080/10408440802272158. View

Mie A, Ruden C, Grandjean P . Safety of Safety Evaluation of Pesticides: developmental neurotoxicity of chlorpyrifos and chlorpyrifos-methyl. Environ Health. 2018; 17(1):77. PMC: 6238321. DOI: 10.1186/s12940-018-0421-y. View

El-Nahhal Y, Lubbad R . Acute and single repeated dose effects of low concentrations of chlorpyrifos, diuron, and their combination on chicken. Environ Sci Pollut Res Int. 2018; 25(11):10837-10847. DOI: 10.1007/s11356-018-1313-y. View

Carr R, Alugubelly N, de Leon K, Loyant L, Mohammed A, Patterson M . Inhibition of fatty acid amide hydrolase by chlorpyrifos in juvenile rats results in altered exploratory and social behavior as adolescents. Neurotoxicology. 2020; 77:127-136. PMC: 7986983. DOI: 10.1016/j.neuro.2020.01.002. View

Shenouda J, Green P, Sultatos L . An evaluation of the inhibition of human butyrylcholinesterase and acetylcholinesterase by the organophosphate chlorpyrifos oxon. Toxicol Appl Pharmacol. 2009; 241(2):135-42. PMC: 2790002. DOI: 10.1016/j.taap.2009.08.014. View

Schwantes D, Celso Goncalves Jr A, Conradi Junior E, Campagnolo M, Zimmermann J . Determination of CHLORPYRIFOS by GC/ECD in water and its sorption mechanism study in a RHODIC FERRALSOL. J Environ Health Sci Eng. 2020; 18(1):149-162. PMC: 7203293. DOI: 10.1007/s40201-020-00448-1. View

Zhong J, Shen D, Li H, He Y, Bao Q, Wang W . Fate of chlorpyrifos bound residues in paddy soils: Release, transformation, and phytoavailability. Environ Int. 2022; 166:107338. DOI: 10.1016/j.envint.2022.107338. View

Zarate L, Pontillo C, Espanol A, Miret N, Chiappini F, Cocca C . Angiogenesis signaling in breast cancer models is induced by hexachlorobenzene and chlorpyrifos, pesticide ligands of the aryl hydrocarbon receptor. Toxicol Appl Pharmacol. 2020; 401:115093. DOI: 10.1016/j.taap.2020.115093. View

Brancato A, Brocca D, De Lentdecker C, Erdos Z, Ferreira L, Greco L . Review of the existing maximum residue levels for chlorpyrifos according to Article 12 of Regulation (EC) No 396/2005. EFSA J. 2020; 15(3):e04733. PMC: 7009835. DOI: 10.2903/j.efsa.2017.4733. View

10.

Akoto O, Gavor S, Appah M, Apau J . Estimation of human health risk associated with the consumption of pesticide-contaminated vegetables from Kumasi, Ghana. Environ Monit Assess. 2015; 187(5):244. DOI: 10.1007/s10661-015-4471-0. View

11.

Parasuraman S . Toxicological screening. J Pharmacol Pharmacother. 2011; 2(2):74-9. PMC: 3127354. DOI: 10.4103/0976-500X.81895. View

12.

Venerosi A, Tait S, Stecca L, Chiarotti F, de Felice A, Cometa M . Effects of maternal chlorpyrifos diet on social investigation and brain neuroendocrine markers in the offspring - a mouse study. Environ Health. 2015; 14:32. PMC: 4448273. DOI: 10.1186/s12940-015-0019-6. View

13.

Rayu S, Nielsen U, Nazaries L, Singh B . Isolation and Molecular Characterization of Novel Chlorpyrifos and 3,5,6-trichloro-2-pyridinol-degrading Bacteria from Sugarcane Farm Soils. Front Microbiol. 2017; 8:518. PMC: 5378769. DOI: 10.3389/fmicb.2017.00518. View

14.

Atabila A, Phung D, Hogarh J, Osei-Fosu P, Sadler R, Connell D . Dermal exposure of applicators to chlorpyrifos on rice farms in Ghana. Chemosphere. 2017; 178:350-358. DOI: 10.1016/j.chemosphere.2017.03.062. View

15.

Shaker E, Elsharkawy E . Organochlorine and organophosphorus pesticide residues in raw buffalo milk from agroindustrial areas in Assiut, Egypt. Environ Toxicol Pharmacol. 2015; 39(1):433-40. DOI: 10.1016/j.etap.2014.12.005. View

16.

Mehta A, Verma R, Srivastava N . Chlorpyrifos-induced DNA damage in rat liver and brain. Environ Mol Mutagen. 2008; 49(6):426-33. DOI: 10.1002/em.20397. View

17.

Kousba A, Sultatos L, Poet T, Timchalk C . Comparison of chlorpyrifos-oxon and paraoxon acetylcholinesterase inhibition dynamics: potential role of a peripheral binding site. Toxicol Sci. 2004; 80(2):239-48. DOI: 10.1093/toxsci/kfh163. View

18.

Erhirhie E, Ihekwereme C, Ilodigwe E . Advances in acute toxicity testing: strengths, weaknesses and regulatory acceptance. Interdiscip Toxicol. 2018; 11(1):5-12. PMC: 6117820. DOI: 10.2478/intox-2018-0001. View

19.

Kopjar N, Zunec S, Mendas G, Micek V, Kasuba V, Mikolic A . Evaluation of chlorpyrifos toxicity through a 28-day study: Cholinesterase activity, oxidative stress responses, parent compound/metabolite levels, and primary DNA damage in blood and brain tissue of adult male Wistar rats. Chem Biol Interact. 2017; 279:51-63. DOI: 10.1016/j.cbi.2017.10.029. View

20.

Slotkin T . Does early-life exposure to organophosphate insecticides lead to prediabetes and obesity?. Reprod Toxicol. 2010; 31(3):297-301. PMC: 3025269. DOI: 10.1016/j.reprotox.2010.07.012. View