Redox and Biometal Status in Wistar Rats After Subacute Exposure to Fluoride and Selenium Counter-effects

Overview

Journal Arh Hig Rada Toksikol

Specialty Occupational Medicine

Date 2022 Oct 13

PMID 36226821

Authors

Jelena Radovanovic

Biljana Antonijevic

Katarina Baralic

Marijana Curcic

Danijela dukic-Cosic

Zorica Bulat

Dragana Javorac

Aleksandra Buha dordevic

Jelena Kotur-Stevuljevic

Emina Sudar-Milovanovic

Evica Antonijevic Miljakovic

Milos Beloica

Zoran Mandinic

Affiliations

Soon will be listed here.

Abstract

This study aimed to investigate the effect of 150 mg/L sodium fluoride (NaF) on redox status parameters and essential metals [copper (Cu), iron (Fe), and zinc (Zn)] in the blood, liver, kidney, brain, and spleen of Wistar rats and to determine the protective potential of selenium (Se) against fluoride (F) toxicity. Male Wistar rats were randomly distributed in groups of five (n=5) receiving tap water (control) or water with NaF 150 mg/L, NaF 150 mg/L + Se 1.5 mg/L, and Se 1.5 mg/L solutions for 28 days. Fluorides caused an imbalance in the redox and biometal (Cu, Fe, and Zn) status, leading to high superoxide anion (O) and malondialdehyde (MDA) levels in the blood and brain and a drop in superoxide dismutase (SOD1) activity in the liver and its increase in the brain and kidneys. Se given with NaF improved MDA, SOD1, and O in the blood, brain, and kidneys, while alone it decreased SH group levels in the liver and kidney. Biometals both reduced and increased F- toxicity. Further research is needed before Se should be considered as a promising strategy for mitigating F toxicity.

References

Basha P, Madhusudhan N . Pre and post natal exposure of fluoride induced oxidative macromolecular alterations in developing central nervous system of rat and amelioration by antioxidants. Neurochem Res. 2010; 35(7):1017-28. DOI: 10.1007/s11064-010-0150-2. View

Hubrecht R, Carter E . The 3Rs and Humane Experimental Technique: Implementing Change. Animals (Basel). 2019; 9(10). PMC: 6826930. DOI: 10.3390/ani9100754. View

Mou S, Qin S, Hu Q, Duan X . [Effect of fluorine, selenium and cadmium on anti-oxidase and microelements in rat's body]. Wei Sheng Yan Jiu. 2004; 33(2):211-3. View

Guo Q, Sun Z, Niu R, Kumar Manthari R, Yuan M, Yang K . Effect of arsenic and/or fluoride gestational exposure on renal autophagy in offspring mice. Chemosphere. 2019; 241:124861. DOI: 10.1016/j.chemosphere.2019.124861. View

Mondal P, Kumar Purkait M . Preparation and characterization of novel green synthesized iron-aluminum nanocomposite and studying its efficiency in fluoride removal. Chemosphere. 2019; 235:391-402. DOI: 10.1016/j.chemosphere.2019.06.189. View

Yuksek V, Cetin S, Usta A . The effect of vitamin E and selenium combination in repairing fluoride-induced DNA damage to NRK-52E cells. Mol Biol Rep. 2020; 47(10):7761-7770. DOI: 10.1007/s11033-020-05852-2. View

Haldar D, Duarah P, Kumar Purkait M . MOFs for the treatment of arsenic, fluoride and iron contaminated drinking water: A review. Chemosphere. 2020; 251:126388. DOI: 10.1016/j.chemosphere.2020.126388. View

Chen J, Cao J, Luo Y, Li J . [Effects of nano-selenium on antioxidant capacity and histopathology of Cyprinus carpio liver under fluoride stress]. Ying Yong Sheng Tai Xue Bao. 2014; 24(10):2970-6. View

Chmielnicka J, Bem E, Brzeznicka E, KASPEREK M . The tissue disposition of zinc and copper following repeated administration of cadmium and selenium to rats. Environ Res. 1985; 37(2):419-24. DOI: 10.1016/0013-9351(85)90123-9. View

10.

Ma Y, Ma Z, Yin S, Yan X, Wang J . Arsenic and fluoride induce apoptosis, inflammation and oxidative stress in cultured human umbilical vein endothelial cells. Chemosphere. 2016; 167:454-461. DOI: 10.1016/j.chemosphere.2016.10.025. View

11.

Daston G, Rehnberg B, Carver B, Kavlock R . Toxicity of sodium fluoride to the postnatally developing rat kidney. Environ Res. 1985; 37(2):461-74. DOI: 10.1016/0013-9351(85)90128-8. View

12.

Korkmaz R, Yuksek V, Dede S . The Effects of Sodium Fluoride (NaF) Treatment on the PI3K/Akt Signal Pathway in NRK-52E Cells. Biol Trace Elem Res. 2021; 200(7):3294-3302. DOI: 10.1007/s12011-021-02927-4. View

13.

Ellman G . Tissue sulfhydryl groups. Arch Biochem Biophys. 1959; 82(1):70-7. DOI: 10.1016/0003-9861(59)90090-6. View

14.

Maheshwari N, Qasim N, Anjum R, Mahmood R . Fluoride enhances generation of reactive oxygen and nitrogen species, oxidizes hemoglobin, lowers antioxidant power and inhibits transmembrane electron transport in isolated human red blood cells. Ecotoxicol Environ Saf. 2021; 208:111611. DOI: 10.1016/j.ecoenv.2020.111611. View

15.

Barbier O, Arreola-Mendoza L, Del Razo L . Molecular mechanisms of fluoride toxicity. Chem Biol Interact. 2010; 188(2):319-33. DOI: 10.1016/j.cbi.2010.07.011. View

16.

Shuhua X, Ziyou L, Ling Y, Fei W, Sun G . A role of fluoride on free radical generation and oxidative stress in BV-2 microglia cells. Mediators Inflamm. 2012; 2012:102954. PMC: 3425889. DOI: 10.1155/2012/102954. View

17.

Alencar de Oliveira Lima L, Miranda G, Aragao W, Bittencourt L, Santos S, Platini Caldas de Souza M . Effects of Fluoride on Submandibular Glands of Mice: Changes in Oxidative Biochemistry, Proteomic Profile, and Genotoxicity. Front Pharmacol. 2021; 12:715394. PMC: 8503261. DOI: 10.3389/fphar.2021.715394. View

18.

Balaha M, Ahmed N, Geddawy A, Kandeel S . Fraxetin prevented sodium fluoride-induced chronic pancreatitis in rats: Role of anti-inflammatory, antioxidant, antifibrotic and anti-apoptotic activities. Int Immunopharmacol. 2021; 93:107372. DOI: 10.1016/j.intimp.2021.107372. View

19.

Wang H, Zhao W, Liu J, Tan P, Zhang C, Zhou B . Fluoride-induced oxidative stress and apoptosis are involved in the reducing of oocytes development potential in mice. Chemosphere. 2017; 186:911-918. DOI: 10.1016/j.chemosphere.2017.08.068. View

20.

Girotti M, Khan N, McLellan B . Early measurement of systemic lipid peroxidation products in the plasma of major blunt trauma patients. J Trauma. 1991; 31(1):32-5. DOI: 10.1097/00005373-199101000-00007. View