Antiproliferative Activity of Green Synthesised Silver Nanoparticles of Cassia Marginata Roxb

Overview

Journal Appl Biochem Biotechnol

Specialties Biochemistry
Biotechnology

Date 2022 Oct 10

PMID 36214952

Authors

Balasubramanian Arul

Ramalingam Kothai

Venkattappan Anbazhagan

Affiliations

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Abstract

Green synthesised silver nanoparticles (AgNPs) have been identified as a promising new therapy pathway for cancer because of their anti-angiogenic potency. In the present study, CM-AgNPs were biosynthesized using aqueous leaf extract of Cassia marginata Roxb (CM) and were confirmed by spectral studies like UV-Visible and attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopic techniques. The structure and shape of the biosynthesized CM-AgNPs were confirmed by scanning electron microscope (SEM), energy-dispersive spectroscopy (EDX), and high-resolution transmission electron microscopy (HR-TEM) with selected area electron diffraction (SAED) studies. The antioxidant activity of the synthesized nanoparticles were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assay methods, and the IC values were found to be 19.58 ± 1.62 and 742.33 ± 14.57 µg, respectively. The anticancer activity of CM-AgNPs was evaluated against human breast cancer cell lines MCF-7 and MDA-MB-231 by 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, and the IC values were found to be 21.02 ± 1.67 and 26.21 ± 0.33 μg/ml, respectively. These results provide promising information about the biosynthesized CM-AgNPs that can be used in the development of novel anticancer drugs.

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