RhCNB Improves Cyclophosphamide-Induced Immunodeficiency in BALB/c Mice

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2022 Oct 7

PMID 36204132

Authors

Wenhua Zhong

Hui Huang

Zhaoxin Yang

Penghuan Chang

Affiliations

Soon will be listed here.

Abstract

Background: This study aims to explore the immunomodulatory effect of rhCNB on mice with cyclophosphamide (CTX)-induced immunodeficiency through TLR/MAPK pathway.

Methods: BALB/c mice were randomly divided into three groups: a negative control group, an immunosuppression model group, and a rhCNB treatment group. Tail vein injection of cyclophosphamide (40 mg/kg) was used to establish a mouse immunosuppression model. Intraperitoneal injection of rhCNB (20 mg/kg) was administered to the treatment group, whereas equal quantities of normal saline were given to the control group and model group. Perform peripheral blood routine of CD4, CD8, and CD19 lymphocyte subsets and peripheral blood Th1/Th2 cell subsets 24 hours after the last administration. RT-PCR was used to detect mRNA levels of TLR, P38, JNK, T-bet, and GATA, the spleen immune organ index was measured, and the histopathological status of the spleen and thymus was observed.

Results: The results showed that compared with the control group, WBC, PLT, LYM, NEU, immune organ index, CD4/CD8 and CD19 subgroup ratio, and peripheral blood Th1/Th2 cell subgroups decreased in the model group. The mRNA levels of TLR, P38, JNK, T-bet, and GATA decreased compared with the model group, while they increased in the treatment group.

Conclusions: rhCNB has an immunomodulatory effect by regulating the expression of Th1/Th2 cytokine balance through the TLR/MAPK signaling pathway and promoting the differentiation and proliferation of lymphocytes, thereby improving the immune function.

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