Engineering Synthetic Microbial Consortium for Cadaverine Biosynthesis from Glycerol

Overview

Journal Biotechnol Lett

Specialties Biochemistry
Biotechnology

Date 2022 Oct 6

PMID 36203106

Authors

Simin Liu

Jiali Mi

Kejing Song

Haishan Qi

Lei Zhang

Affiliations

Soon will be listed here.

Abstract

Objectives: 1,5-pentanediamine (cadaverine) is a C5 platform chemical, also an important raw material for bio-polyamide PA5X. With increasing concerns about the depletion of fossil resources and global environmental protection, cadaverine bio-production has attracted more attentions.

Results: Here, a microbial consortium consisting of Corynebacterium glutamicum cgl-FDK and Escherichia coli BL-ABST-Spy was constructed to de novo synthesize cadaverine utilizing glycerol as the sole carbon resource. The glycerol utilization pathway was initially constructed in C. glutamicum cgl-FDK to produce lysine from glycerol. Then, the pyridoxal 5'-phosphate (PLP) biosynthesis pathway and SpyTag/SpyCatcher protein-ligation system for lysine decarboxylase (CadA) and cadaverine-lysine antiporter protein (CadB) were introduced into E. coli BL-ABST-Spy to synthesize cadaverine from lysine. Furthermore, the fermentation conditions of microbial consortium were optimized and the cadaverine production reached 9.3 g/L with glycerol as the sole carbon source.

Conclusions: This work provides a promising strategy for efficiently producing cadaverine from glycerol with an artificial microbial consortium.

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