Efficient Angiogenesis-based Wound Healing Through Hydrogel Dressing with Extracellular Vesicles Release

Overview

Journal Mater Today Bio

Specialty Biomedical Engineering

Date 2022 Oct 4

PMID 36193344

Authors

Zhengzhe Han

Lanlan Dong

Ang Li

Zongyue Li

Landie Fu

Zhichang Zhang

Xiang Li

Xiaolin Li

Affiliations

Soon will be listed here.

Abstract

Wound healing and angiogenesis remain challenges for both clinical and experimental research worldwide. Periosteum-derived extracellular vesicles (P-sEVs) delivered by hydrogel dressings provide a potential strategy for wound defects to promote fast healing. In this study, we designed a NAGA/GelMA/Laponite/glycerol hydrogel wound dressing that can release P-sEVs to accelerate angiogenesis and wound healing (named P-sEVs@hydrogel) (-acryloyl glycinamide, NAGA). The wound dressing showed multiple functions, including efficient angiogenesis, tissue adhesion and a physical barrier. P-sEVs significantly enhanced the proliferation, migration, and tube formation of endothelial cells . The results of experiments showed that P-sEVs@hydrogel accelerates the healing of a full-thickness defect wound model by stimulating the angiogenic process. The improved cell proliferation, tissue formation, remodeling, and re-epithelialization possibly resulted in the fast healing. This study shows that multifunctional hydrogel dressing combined with bioactive molecules can achieve fast and satisfactory wound healing in full-thickness wound defects and other related wounds.

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References

Morelle X, Illeperuma W, Tian K, Bai R, Suo Z, Vlassak J . Highly Stretchable and Tough Hydrogels below Water Freezing Temperature. Adv Mater. 2018; 30(35):e1801541. DOI: 10.1002/adma.201801541. View

Feng Z, Zuo H, Gao W, Ning N, Tian M, Zhang L . A Robust, Self-Healable, and Shape Memory Supramolecular Hydrogel by Multiple Hydrogen Bonding Interactions. Macromol Rapid Commun. 2018; 39(20):e1800138. DOI: 10.1002/marc.201800138. View

Debnath S, Yallowitz A, McCormick J, Lalani S, Zhang T, Xu R . Discovery of a periosteal stem cell mediating intramembranous bone formation. Nature. 2018; 562(7725):133-139. PMC: 6193396. DOI: 10.1038/s41586-018-0554-8. View

Janpetch N, Saito N, Rujiravanit R . Fabrication of bacterial cellulose-ZnO composite via solution plasma process for antibacterial applications. Carbohydr Polym. 2016; 148:335-44. DOI: 10.1016/j.carbpol.2016.04.066. View

Zhao X, Li P, Guo B, Ma P . Antibacterial and conductive injectable hydrogels based on quaternized chitosan-graft-polyaniline/oxidized dextran for tissue engineering. Acta Biomater. 2015; 26:236-48. DOI: 10.1016/j.actbio.2015.08.006. View

Saleh B, Dhaliwal H, Portillo-Lara R, Sani E, Abdi R, Amiji M . Local Immunomodulation Using an Adhesive Hydrogel Loaded with miRNA-Laden Nanoparticles Promotes Wound Healing. Small. 2019; 15(36):e1902232. PMC: 6726510. DOI: 10.1002/smll.201902232. View

Ortinau L, Lei K, Jeong Y, Park D . Real-Time Imaging of CCL5-Induced Migration of Periosteal Skeletal Stem Cells in Mice. J Vis Exp. 2020; (163). PMC: 9119154. DOI: 10.3791/61162. View

Sulaeva I, Hettegger H, Bergen A, Rohrer C, Kostic M, Konnerth J . Fabrication of bacterial cellulose-based wound dressings with improved performance by impregnation with alginate. Mater Sci Eng C Mater Biol Appl. 2020; 110:110619. DOI: 10.1016/j.msec.2019.110619. View

Armstrong D, Boulton A, Bus S . Diabetic Foot Ulcers and Their Recurrence. N Engl J Med. 2017; 376(24):2367-2375. DOI: 10.1056/NEJMra1615439. View

10.

Zeng R, Lin C, Lin Z, Chen H, Lu W, Lin C . Approaches to cutaneous wound healing: basics and future directions. Cell Tissue Res. 2018; 374(2):217-232. DOI: 10.1007/s00441-018-2830-1. View

11.

Page D, Clarkin C, Mani R, Khan N, Dawson J, Evans N . Injectable nanoclay gels for angiogenesis. Acta Biomater. 2019; 100:378-387. DOI: 10.1016/j.actbio.2019.09.023. View

12.

Peng Y, He D, Ge X, Lu Y, Chai Y, Zhang Y . Construction of heparin-based hydrogel incorporated with Cu5.4O ultrasmall nanozymes for wound healing and inflammation inhibition. Bioact Mater. 2021; 6(10):3109-3124. PMC: 7960791. DOI: 10.1016/j.bioactmat.2021.02.006. View

13.

Jones R, Foster D, Longaker M . Management of Chronic Wounds-2018. JAMA. 2018; 320(14):1481-1482. DOI: 10.1001/jama.2018.12426. View

14.

Lim H, Kim H, Qazi R, Kwon Y, Jeong J, Yeo W . Advanced Soft Materials, Sensor Integrations, and Applications of Wearable Flexible Hybrid Electronics in Healthcare, Energy, and Environment. Adv Mater. 2019; 32(15):e1901924. DOI: 10.1002/adma.201901924. View

15.

Song S, Liu Z, Abubaker M, Ding L, Zhang J, Yang S . Antibacterial polyvinyl alcohol/bacterial cellulose/nano-silver hydrogels that effectively promote wound healing. Mater Sci Eng C Mater Biol Appl. 2021; 126:112171. DOI: 10.1016/j.msec.2021.112171. View

16.

Manchineella S, Thrivikraman G, Khanum K, Ramamurthy P, Basu B, Govindaraju T . Pigmented Silk Nanofibrous Composite for Skeletal Muscle Tissue Engineering. Adv Healthc Mater. 2016; 5(10):1222-32. DOI: 10.1002/adhm.201501066. View

17.

Han S, Peng H, Sun Q, Venkatesh S, Chung K, Lau S . An Overview of the Development of Flexible Sensors. Adv Mater. 2017; 29(33). DOI: 10.1002/adma.201700375. View

18.

Serowoky M, Arata C, Crump J, Mariani F . Skeletal stem cells: insights into maintaining and regenerating the skeleton. Development. 2020; 147(5). PMC: 7075071. DOI: 10.1242/dev.179325. View

19.

Ortinau L, Wang H, Lei K, Deveza L, Jeong Y, Hara Y . Identification of Functionally Distinct Mx1+αSMA+ Periosteal Skeletal Stem Cells. Cell Stem Cell. 2019; 25(6):784-796.e5. PMC: 7055207. DOI: 10.1016/j.stem.2019.11.003. View

20.

Deveza L, Ortinau L, Lei K, Park D . Comparative analysis of gene expression identifies distinct molecular signatures of bone marrow- and periosteal-skeletal stem/progenitor cells. PLoS One. 2018; 13(1):e0190909. PMC: 5771600. DOI: 10.1371/journal.pone.0190909. View