Potential Molecular Mechanism of Guishen Huoxue Decoction Against Intrauterine Adhesion Based on Network Pharmacology

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2022 Oct 4

PMID 36193142

Authors

Wenyan Zhang

Yuan Yuan

Guangrong Huang

Jing Xiao

Affiliations

Soon will be listed here.

Abstract

Objective: Intrauterine adhesion (IUA) represents an endometrial repair disorder that is associated with menstrual disorders, recurrent pregnancy loss, and infertility. This study aimed to explore the underlying biological mechanisms of Guishen Huoxue decoction for the treatment of IUA based on network pharmacology.

Methods: The selection of active compounds for Guishen Huoxue decoction and prediction of relevant targets were performed by the TCMSP and Swiss Target Prediction databases, respectively. The targets of IUA were obtained by three databases, including Online Mendelian Inheritance in Man (OMIM), DisGeNET, and GeneCards. The drug-disease regulatory network was constructed via Cytoscape software, following the acquisition of common genes of active compounds of drug Guishen Huoxue decoction and disease IUA, which was carried out through Venny software. Protein-protein interaction (PPI) network and function enrichment analyses were performed.

Results: According to the data obtained from TCMSP, a total of 200 potential active compounds of Guishen Huoxue decoction and their related targets (1068) were screened by the Swiss Target Prediction database. 1303 disease targets and 134 common targets were identified. The drug-disease regulatory network showed that 165 active compounds were found to be involved in the treatment of IUA. Among 134 common targets, AKT1, SRC, TP53, VEGFA, and IL-6 were predicted as core genes against IUA. PI3K-Akt, Rap1, Ras, and AGE-RAGE were the main signaling pathways that participated in the treatment of Guishen Huoxue decoction for IUA.

Conclusion: The active compounds of Guishen Huoxue decoction confer therapeutic effects against IUA by regulating fibrosis, inflammation, and oxidative stress through major signaling pathways such as PI3K-Akt and AGE-RAGE.

Citing Articles

Integrating Network Pharmacology and In Vitro Experimental Verification Revealing Bushenhuoxue Recipe Against Intrauterine Adhesions via PI3K-AKT Signaling Pathway.

Wang L, Zhuang L, Lu Y, Jia J, Chen C, Zhang Y Biochem Genet. 2024; 63(1):686-697.

PMID: 38502457 DOI: 10.1007/s10528-024-10732-6.

Retracted: Potential Molecular Mechanism of Guishen Huoxue Decoction against Intrauterine Adhesion Based on Network Pharmacology.

And Alternative Medicine E Evid Based Complement Alternat Med. 2023; 2023:9803084.

PMID: 38125121 PMC: 10732954. DOI: 10.1155/2023/9803084.

HPV infection and vaginal microecological disorders in women with intrauterine adhesion: cross-sectional study in a Chinese population.

Wang L, Zeng J, Liu H, Xu H, Liu Y, Luo M BMC Infect Dis. 2023; 23(1):836.

PMID: 38012631 PMC: 10683081. DOI: 10.1186/s12879-023-08659-1.

Engineering self-healing adhesive hydrogels with antioxidant properties for intrauterine adhesion prevention.

Feng L, Wang L, Ma Y, Duan W, Martin-Saldana S, Zhu Y Bioact Mater. 2023; 27:82-97.

PMID: 37006827 PMC: 10063385. DOI: 10.1016/j.bioactmat.2023.03.013.

References

Niu H, Miao X, Zhan X, Zhou X, Li X, Jiang L . Tiaoshen Tongluo Attenuates Fibrosis by Modulating the TGF-1/Smad Pathway in Endometrial Stromal Cells and a Rat Model of Intrauterine Adhesion. Evid Based Complement Alternat Med. 2021; 2021:6675329. PMC: 8088367. DOI: 10.1155/2021/6675329. View

Roy K, Baruah J, Sharma J, Kumar S, Kachawa G, Singh N . Reproductive outcome following hysteroscopic adhesiolysis in patients with infertility due to Asherman's syndrome. Arch Gynecol Obstet. 2009; 281(2):355-61. DOI: 10.1007/s00404-009-1117-x. View

Aleshin A, Finn R . SRC: a century of science brought to the clinic. Neoplasia. 2010; 12(8):599-607. PMC: 2915404. DOI: 10.1593/neo.10328. View

Yu D, Li T, Xia E, Huang X, Liu Y, Peng X . Factors affecting reproductive outcome of hysteroscopic adhesiolysis for Asherman's syndrome. Fertil Steril. 2007; 89(3):715-22. DOI: 10.1016/j.fertnstert.2007.03.070. View

Chiou G, Yan H, Lei X, Li B, Shen Z . Ocular and cardiovascular pharmacology of tetramethylpyrazine isolated from Ligusticum wallichii Franch. Zhongguo Yao Li Xue Bao. 1991; 12(2):99-104. View

Nie Y, Hu Y, Yu K, Zhang D, Shi Y, Li Y . Akt1 regulates pulmonary fibrosis via modulating IL-13 expression in macrophages. Innate Immun. 2019; 25(7):451-461. PMC: 6900639. DOI: 10.1177/1753425919861774. View

DeMayo F, Zhao B, Takamoto N, Tsai S . Mechanisms of action of estrogen and progesterone. Ann N Y Acad Sci. 2002; 955:48-59; discussion 86-8, 396-406. DOI: 10.1111/j.1749-6632.2002.tb02765.x. View

Azumaguchi A, Henmi H, Saito T . Efficacy of silicone sheet as a personalized barrier for preventing adhesion reformation after hysteroscopic adhesiolysis of intrauterine adhesions. Reprod Med Biol. 2019; 18(4):378-383. PMC: 6780041. DOI: 10.1002/rmb2.12294. View

Tanaka T, Narazaki M, Kishimoto T . IL-6 in inflammation, immunity, and disease. Cold Spring Harb Perspect Biol. 2014; 6(10):a016295. PMC: 4176007. DOI: 10.1101/cshperspect.a016295. View

10.

Song Y, Liu P, Tan J, Zou C, Li Q, Li-Ling J . Stem cell-based therapy for ameliorating intrauterine adhesion and endometrium injury. Stem Cell Res Ther. 2021; 12(1):556. PMC: 8557001. DOI: 10.1186/s13287-021-02620-2. View

11.

Berman J . Intrauterine adhesions. Semin Reprod Med. 2008; 26(4):349-55. DOI: 10.1055/s-0028-1082393. View

12.

Shah S, Brock E, Ji K, Mattingly R . Ras and Rap1: A tale of two GTPases. Semin Cancer Biol. 2018; 54:29-39. PMC: 6170734. DOI: 10.1016/j.semcancer.2018.03.005. View

13.

Yu J, Jiang L, Gao Y, Sun Q, Liu B, Hu Y . Interaction between BMSCs and EPCs promotes IUA angiogenesis via modulating PI3K/Akt/Cox2 axis. Am J Transl Res. 2019; 10(12):4280-4289. PMC: 6325499. View

14.

Leroy B, Anderson M, Soussi T . TP53 mutations in human cancer: database reassessment and prospects for the next decade. Hum Mutat. 2014; 35(6):672-88. DOI: 10.1002/humu.22552. View

15.

Dreisler E, Kjer J . Asherman's syndrome: current perspectives on diagnosis and management. Int J Womens Health. 2019; 11:191-198. PMC: 6430995. DOI: 10.2147/IJWH.S165474. View

16.

Bai X, Liu J, Cao S, Wang L . Mechanisms of endometrial fibrosis and the potential application of stem cell therapy. Discov Med. 2019; 27(150):267-279. View

17.

Ai Y, Chen M, Liu J, Ren L, Yan X, Feng Y . lncRNA TUG1 promotes endometrial fibrosis and inflammation by sponging miR-590-5p to regulate Fasl in intrauterine adhesions. Int Immunopharmacol. 2020; 86:106703. DOI: 10.1016/j.intimp.2020.106703. View

18.

Xu Q, Duan H, Gan L, Liu X, Chen F, Shen X . MicroRNA-1291 promotes endometrial fibrosis by regulating the ArhGAP29-RhoA/ROCK1 signaling pathway in a murine model. Mol Med Rep. 2017; 16(4):4501-4510. PMC: 5647010. DOI: 10.3892/mmr.2017.7210. View

19.

Reske J, Wilson M, Holladay J, Siwicki R, Skalski H, Harkins S . Co-existing TP53 and ARID1A mutations promote aggressive endometrial tumorigenesis. PLoS Genet. 2021; 17(12):e1009986. PMC: 8741038. DOI: 10.1371/journal.pgen.1009986. View

20.

Pang W, Zhang Q, Ding H, Sun N, Li W . Effect of new biological patch in repairing intrauterine adhesion and improving clinical pregnancy outcome in infertile women: study protocol for a randomized controlled trial. Trials. 2022; 23(1):510. PMC: 9206332. DOI: 10.1186/s13063-022-06428-0. View