Crypt-Villus Scaffold Architecture for Bioengineering Functional Human Intestinal Epithelium

Overview

Journal ACS Biomater Sci Eng

Publisher American Chemical Society

Specialties Biomedical Engineering
Biotechnology

Date 2022 Oct 3

PMID 36191009

Authors

Sara E Rudolph

Brooke N Longo

Megan W Tse

Megan R Houchin

Mina M Shokoufandeh

Ying Chen

David L Kaplan

Affiliations

Soon will be listed here.

Abstract

Crypt-villus architecture in the small intestine is crucial for the structural integrity of the intestinal epithelium and maintenance of gut homeostasis. We utilized three-dimensional (3D) printing and inverse molding techniques to form three-dimensional (3D) spongy scaffold systems that resemble the intestinal crypt-villus microarchitecture. The scaffolds consist of silk fibroin protein with curved lumens with rows of protruding villi with invaginating crypts to generate the architecture. Intestinal cell (Caco-2, HT29-MTX) attachment and growth, as well as long-term culture support were demonstrated with cell polarization and tissue barrier properties compared to two-dimensional (2D) Transwell culture controls. Further, physiologically relevant oxygen gradients were generated in the 3D system. The various advantages of this system may be ascribed to the more physiologically relevant 3D environment, offering a system for the exploration of disease pathogenesis, host-microbiome interactions, and therapeutic discovery.

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