Evaluation of Electrolyte Element Composition in Human Tissue by Laser-induced Breakdown Spectroscopy (LIBS)

Overview

Journal Sci Rep

Specialty Science

Date 2022 Sep 30

PMID 36180727

Authors

Philipp Winnand

K Olaf Boernsen

Georgi Bodurov

Matthias Lammert

Frank Holzle

Ali Modabber

Affiliations

Soon will be listed here.

Abstract

Laser-induced breakdown spectroscopy (LIBS) enables the direct measurement of cell electrolyte concentrations. The utility of LIBS spectra in biomarker studies is limited because these studies rarely consider basic physical principles. The aim of this study was to test the suitability of LIBS spectra as an analytical method for biomarker assays and to evaluate the composition of electrolyte elements in human biomaterial. LIBS as an analytical method was evaluated by establishing KCl calibration curves to demonstrate linearity, by the correct identification of emission lines with corresponding reference spectra, and by the feasibility to use LIBS in human biomaterial, analyzing striated muscle tissues from the oral regions of two patients. Lorentzian peak fit and peak area calculations resulted in better linearity and reduced shot-to-shot variance. Correct quantitative measurement allowed for differentiation of human biomaterial between patients, and determination of the concentration ratios of main electrolytes within human tissue. The clinical significance of LIBS spectra should be evaluated using peak area rather than peak intensity. LIBS might be a promising tool for analyzing a small group of living cells. Due to linearity, specificity and robustness of the proposed analytical method, LIBS could be a component of future biomarker studies.

Citing Articles

Assessment of the bony resection margin distance in bone-invasive oral cancer using laser-induced breakdown spectroscopy.

Winnand P, Ooms M, Heitzer M, Vohl N, Lammert M, Holzle F Clin Oral Investig. 2024; 28(9):474.

PMID: 39112646 PMC: 11306308. DOI: 10.1007/s00784-024-05862-5.

Assessment of the Electrolyte Heterogeneity of Tissues in Mandibular Bone-Infiltrating Head and Neck Cancer Using Laser-Induced Breakdown Spectroscopy.

Winnand P, Boernsen K, Ooms M, Heitzer M, Vohl N, Lammert M Int J Mol Sci. 2024; 25(5).

PMID: 38473853 PMC: 10932450. DOI: 10.3390/ijms25052607.

The role of potassium in depth profiling of the tumor border in bone-invasive oral cancer using laser-induced breakdown spectroscopy (LIBS): a pilot study.

Winnand P, Boernsen K, Ooms M, Heitzer M, Lammert M, Eschweiler J J Cancer Res Clin Oncol. 2023; 149(18):16635-16645.

PMID: 37716922 PMC: 10645631. DOI: 10.1007/s00432-023-05411-9.

References

Tian Y, Hou S, Wang L, Duan X, Xue B, Lu Y . CaOH Molecular Emissions in Underwater Laser-Induced Breakdown Spectroscopy: Spatial-Temporal Characteristics and Analytical Performances. Anal Chem. 2019; 91(21):13970-13977. DOI: 10.1021/acs.analchem.9b03513. View

Mehari F, Rohde M, Knipfer C, Kanawade R, Klampfl F, Adler W . Laser induced breakdown spectroscopy for bone and cartilage differentiation - ex vivo study as a prospect for a laser surgery feedback mechanism. Biomed Opt Express. 2014; 5(11):4013-23. PMC: 4242035. DOI: 10.1364/BOE.5.004013. View

Peng J, He Y, Ye L, Shen T, Liu F, Kong W . Moisture Influence Reducing Method for Heavy Metals Detection in Plant Materials Using Laser-Induced Breakdown Spectroscopy: A Case Study for Chromium Content Detection in Rice Leaves. Anal Chem. 2017; 89(14):7593-7600. DOI: 10.1021/acs.analchem.7b01441. View

Morton A, Norton C, Jacobsen N, Fernando C, Cornelison D, Segal S . Barium chloride injures myofibers through calcium-induced proteolysis with fragmentation of motor nerves and microvessels. Skelet Muscle. 2019; 9(1):27. PMC: 6833148. DOI: 10.1186/s13395-019-0213-2. View

Alfarouk K, B M Ahmed S, Ahmed A, Elliott R, Ibrahim M, Ali H . The Interplay of Dysregulated pH and Electrolyte Imbalance in Cancer. Cancers (Basel). 2020; 12(4). PMC: 7226178. DOI: 10.3390/cancers12040898. View

Sun C, Xu W, Tan Y, Zhang Y, Yue Z, Zou L . From machine learning to transfer learning in laser-induced breakdown spectroscopy analysis of rocks for Mars exploration. Sci Rep. 2021; 11(1):21379. PMC: 8560777. DOI: 10.1038/s41598-021-00647-2. View

Zhang B, Yu H, Sun L, Xin Y, Cong Z . A method for resolving overlapped peaks in laser-induced breakdown spectroscopy (LIBS). Appl Spectrosc. 2013; 67(9):1087-97. DOI: 10.1366/12-06822. View

Guerenne-Del Ben T, Couderc V, Duponchel L, Sol V, Leproux P, Petit J . Multiplex coherent anti-Stokes Raman scattering microspectroscopy detection of lipid droplets in cancer cells expressing TrkB. Sci Rep. 2020; 10(1):16749. PMC: 7542145. DOI: 10.1038/s41598-020-74021-z. View

Sancey L, Motto-Ros V, Busser B, Kotb S, Benoit J, Piednoir A . Laser spectrometry for multi-elemental imaging of biological tissues. Sci Rep. 2014; 4:6065. PMC: 4206947. DOI: 10.1038/srep06065. View

10.

Yue Z, Sun C, Chen F, Zhang Y, Xu W, Shabbir S . Machine learning-based LIBS spectrum analysis of human blood plasma allows ovarian cancer diagnosis. Biomed Opt Express. 2021; 12(5):2559-2574. PMC: 8176811. DOI: 10.1364/BOE.421961. View

11.

Yee L, Lively T, McShane L . Biomarkers in early-phase trials: fundamental issues. Bioanalysis. 2018; 10(12):933-944. PMC: 6123886. DOI: 10.4155/bio-2018-0006. View

12.

Zhang D, Hu Z, Su Y, Hai B, Zhu X, Zhu J . Simple method for liquid analysis by laser-induced breakdown spectroscopy (LIBS). Opt Express. 2018; 26(14):18794-18802. DOI: 10.1364/OE.26.018794. View

13.

Saudemont P, Quanico J, Robin Y, Baud A, Balog J, Fatou B . Real-Time Molecular Diagnosis of Tumors Using Water-Assisted Laser Desorption/Ionization Mass Spectrometry Technology. Cancer Cell. 2018; 34(5):840-851.e4. DOI: 10.1016/j.ccell.2018.09.009. View

14.

Rohde M, Mehari F, Klampfl F, Adler W, Neukam F, Schmidt M . The differentiation of oral soft- and hard tissues using laser induced breakdown spectroscopy - a prospect for tissue specific laser surgery. J Biophotonics. 2016; 10(10):1250-1261. DOI: 10.1002/jbio.201600153. View

15.

Harefa E, Zhou W . Laser-Induced Breakdown Spectroscopy Combined with Nonlinear Manifold Learning for Improvement Aluminum Alloy Classification Accuracy. Sensors (Basel). 2022; 22(9). PMC: 9102175. DOI: 10.3390/s22093129. View

16.

Karnadi I, Pardede M, Tanra I, Hedwig R, Marpaung A, Lie Z . Suppression of self-absorption in laser-induced breakdown spectroscopy using a double pulse orthogonal configuration to create vacuum-like conditions in atmospheric air pressure. Sci Rep. 2020; 10(1):13278. PMC: 7411021. DOI: 10.1038/s41598-020-70151-6. View

17.

NAGY I, Lustyik G, Nagy V, Zarandi B, Bertoni-Freddari C . Intracellular Na+:K+ ratios in human cancer cells as revealed by energy dispersive x-ray microanalysis. J Cell Biol. 1981; 90(3):769-77. PMC: 2111914. DOI: 10.1083/jcb.90.3.769. View

18.

Kanawade R, Mahari F, Klampfl F, Rohde M, Knipfer C, Tangermann-Gerk K . Qualitative tissue differentiation by analysing the intensity ratios of atomic emission lines using laser induced breakdown spectroscopy (LIBS): prospects for a feedback mechanism for surgical laser systems. J Biophotonics. 2013; 8(1-2):153-61. PMC: 4320783. DOI: 10.1002/jbio.201300159. View

19.

Bennet D, Khorsandian Y, Pelusi J, Mirabella A, Pirrotte P, Zenhausern F . Molecular and physical technologies for monitoring fluid and electrolyte imbalance: A focus on cancer population. Clin Transl Med. 2021; 11(6):e461. PMC: 8214861. DOI: 10.1002/ctm2.461. View

20.

Muhammed Shameem K, Dhanada V, Unnikrishnan V, George S, Kartha V, Santhosh C . A hyphenated echelle LIBS-Raman system for multi-purpose applications. Rev Sci Instrum. 2018; 89(7):073108. DOI: 10.1063/1.5024966. View