Distinct Local and Brain-wide Networks Are Activated by Optogenetic Stimulation of Neurons Specific to Each Layer of Motor Cortex

Overview

Journal Neuroimage

Specialty Radiology

Date 2022 Sep 30

PMID 36176220

Authors

Russell W Chan

Greg O Cron

Mazen Asaad

Bradley J Edelman

Hyun Joo Lee

Hillel Adesnik

David Feinberg

Jin Hyung Lee

Affiliations

Soon will be listed here.

Abstract

Primary motor cortex (M1) consists of a stack of interconnected but distinct layers (L1-L6) which affect motor control through large-scale networks. However, the brain-wide functional influence of each layer is poorly understood. We sought to expand our knowledge of these layers' circuitry by combining Cre-driver mouse lines, optogenetics, fMRI, and electrophysiology. Neuronal activities initiated in Drd3 neurons (within L2/3) were mainly confined within M1, while stimulation of Scnn1a, Rbp4, and Ntsr1 neurons (within L4, L5, and L6, respectively) evoked distinct responses in M1 and motor-related subcortical regions, including striatum and motor thalamus. We also found that fMRI responses from targeted stimulations correlated with both local field potentials (LFPs) and spike changes. This study represents a step forward in our understanding of how different layers of primary motor cortex are embedded in brain-wide circuitry.

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