The Effect of High-throughput Vitrification of Human Ovarian Cortex Tissue on Follicular Viability: a Promising Alternative to Conventional Slow Freezing?

Overview

Journal Arch Gynecol Obstet

Specialty Gynecology & Obstetrics

Date 2022 Sep 29

PMID 36175682

Authors

Andreas Schallmoser

Rebekka Einenkel

Cara Farber

Norah Emrich

Julia John

Nicole Sanger

Affiliations

Soon will be listed here.

Abstract

Background: The standard procedure most frequently used for ovarian tissue cryopreservation (OTC) is slow freezing, while vitrification has been proposed as promising alternative and has built an impressive catalog of success in fertility laboratories regarding cryopreservation of oocytes and embryos.

Methods: We developed and evaluated a high-throughput protocol for vitrification of human ovarian tissue suitable for clinical processing. Follicular viability was assessed via calcein staining prior and after cryopreservation analyzing ovarian tissue of a cohort of 30 patients.

Results: We found no significant differences regarding follicular viability between slow frozen and vitrified cortex tissue samples 24 h after thawing and rapid warming. Follicular viability of thawed and rapid warmed samples was not significantly different in comparison to fresh samples, indicating high proportions of follicular survival rates with both methods.

Conclusions: High-throughput vitrification is a promising option in a clinical setting. More research is required to determine the status of other tissue-specific quality indicators potentially influencing on autotransplantation.

Citing Articles

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De novo design of a nanoregulator for the dynamic restoration of ovarian tissue in cryopreservation and transplantation.

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Investigating the impact of vitrification on bovine ovarian tissue morphology, follicle survival, and transcriptomic signature.

Deligiannis S, Kask K, Modhukur V, Boskovic N, Ivask M, Jaakma U J Assist Reprod Genet. 2024; 41(4):1035-1055.

PMID: 38358432 PMC: 11052753. DOI: 10.1007/s10815-024-03038-4.

High FSH levels impair VEGF secretion of human, frozen-thawed ovarian cortical tissue in vitro.

Einenkel R, Schallmoser A, Sanger N Sci Rep. 2024; 14(1):3287.

PMID: 38332226 PMC: 10853201. DOI: 10.1038/s41598-024-53402-8.

Comparison of angiogenic potential in vitrified vs. slow frozen human ovarian tissue.

Schallmoser A, Einenkel R, Farber C, Huren V, Emrich N, John J Sci Rep. 2023; 13(1):12885.

PMID: 37558708 PMC: 10412559. DOI: 10.1038/s41598-023-39920-x.

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