Arrangement of Glycan Chains in the Sacculus of Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1978 Nov 1

PMID 361720

Citations 32

Authors

R W Verwer

N Nanninga

W Keck

U Schwarz

Affiliations

Soon will be listed here.

Abstract

A novel of Escherichia coli endopeptidase was used for a selective partial hydrolysis of the peptide bridges which interlink the glycan chains in E. coli sacculi. The loosening of the murein network revealed, in the electron microscope, a preferential orientation of the glycan chains, more or less perpendicular to the length axis of the cell. Control incubations with E. coli transglycosylase or egg-white lysozyme did not leave ordered structures behind.

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References

WEIDEL W, Frank H, MARTIN H . The rigid layer of the cell wall of Escherichia coli strain B. J Gen Microbiol. 1960; 22:158-66. DOI: 10.1099/00221287-22-1-158. View

WEIDEL W, Pelzer H . BAGSHAPED MACROMOLECULES--A NEW OUTLOOK ON BACTERIAL CELL WALLS. Adv Enzymol Relat Subj Biochem. 1964; 26:193-232. DOI: 10.1002/9780470122716.ch5. View

PRIMOSIGH J, Pelzer H, Maass D, WEIDEL W . Chemical characterization of mucopeptides released from the E. coli B cell wall by enzymic action. Biochim Biophys Acta. 1961; 46:68-80. DOI: 10.1016/0006-3002(61)90647-3. View

Burge R, Fowler A, Reaveley D . Structure of the peptidogylcan of bacterial cell walls. I. J Mol Biol. 1977; 117(4):927-53. DOI: 10.1016/s0022-2836(77)80006-5. View

PREUSSER H . [Structural alterations of the murein sacculus of Spirillum serpens after treatment with uranyl acetate]. Arch Mikrobiol. 1969; 68(2):150-64. View

Ghuysen J . Use of bacteriolytic enzymes in determination of wall structure and their role in cell metabolism. Bacteriol Rev. 1968; 32(4 Pt 2):425-64. PMC: 413160. View

Formanek H, Formanek S, WAWRA H . A three-dimensional atomic model of the murein layer of bacteria. Eur J Biochem. 1974; 46(2):279-94. DOI: 10.1111/j.1432-1033.1974.tb03620.x. View

Braun V, Gnirke H, Henning U, Rehn K . Model for the structure of the shape-maintaining layer of the Escherichia coli cell envelope. J Bacteriol. 1973; 114(3):1264-70. PMC: 285390. DOI: 10.1128/jb.114.3.1264-1270.1973. View

Holtje J, Mirelman D, Sharon N, Schwarz U . Novel type of murein transglycosylase in Escherichia coli. J Bacteriol. 1975; 124(3):1067-76. PMC: 236007. DOI: 10.1128/jb.124.3.1067-1076.1975. View

10.

Hartmann R, Holtje J, Schwarz U . Targets of penicillin action in Escherichia coli. Nature. 1972; 235(5339):426-9. DOI: 10.1038/235426a0. View

11.

Kelemen M, Rogers H . Three-dimensional molecular models of bacterial cell wall mucopeptides (peptidoglycans). Proc Natl Acad Sci U S A. 1971; 68(5):992-6. PMC: 389098. DOI: 10.1073/pnas.68.5.992. View

12.

Schwarz U, Asmus A, Frank H . Autolytic enzymes and cell division of Escherichia coli. J Mol Biol. 1969; 41(3):419-29. DOI: 10.1016/0022-2836(69)90285-x. View

13.

Marr A, Harvey R, Trentini W . Growth and division of Escherichia coli. J Bacteriol. 1966; 91(6):2388-9. PMC: 316226. DOI: 10.1128/jb.91.6.2388-2389.1966. View