Biosynthesis of Copper Oxide Nanoparticles Mediated Annona Muricata As Cytotoxic and Apoptosis Inducer Factor in Breast Cancer Cell Lines

Overview

Journal Sci Rep

Specialty Science

Date 2022 Sep 28

PMID 36171339

Authors

Rana I Mahmood

Afraa Ali Kadhim

Sumayah Ibraheem

Salim Albukhaty

Harraa S Mohammed-Salih

Ruaa H Abbas

Majid S Jabir

Mustafa K A Mohammed

Uday M Nayef

Faizah A AlMalki

Ghassan M Sulaiman

Hassan Al-Karagoly

Affiliations

Soon will be listed here.

Abstract

This study investigated for the first time a simple bio-synthesis approach for the synthesis of copper oxide nanoparticles (CuO NPs) using Annona muricata L (A. muricata) plant extract to test their anti-cancer effects. The presence of CuONPs was confirmed by UV-visible spectroscopy, Scanning electron microscope (SEM), and Transmission electron microscope (TEM). The antiproliferative properties of the synthesized nanoparticles were evaluated against (AMJ-13), (MCF-7) breast cancer cell lines, and the human breast epithelial cell line (HBL-100) as healthy cells. This study indicates that CuONPs reduced cell proliferation for AMJ-13 and MCF-7. HBL-100 cells were not significantly inhibited for several concentration levels or test periods. The outcomes suggest that the prepared copper oxide nanoparticles acted against the growth of specific cell lines observed in breast cancer. It was observed that cancer cells had minor colony creation after 24 h sustained CuONPs exposure using (IC) concentration for AMJ-13 was (17.04 µg mL). While for MCF-7 cells was (18.92 µg mL). It indicates the uptake of CuONPs by cancer cells, triggering apoptosis. Moreover, treatment with CuONPs enhanced Lactate dehydrogenase (LDH) production, probably caused by cell membrane damage, creating leaks comprising cellular substances like lactate dehydrogenase. Hence, research results suggested that the synthesized CuONPs precipitated anti-proliferative effects by triggering cell death through apoptosis.

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References

Sun J, Wang S, Zhao D, Hun F, Weng L, Liu H . Cytotoxicity, permeability, and inflammation of metal oxide nanoparticles in human cardiac microvascular endothelial cells: cytotoxicity, permeability, and inflammation of metal oxide nanoparticles. Cell Biol Toxicol. 2011; 27(5):333-42. DOI: 10.1007/s10565-011-9191-9. View

George V, Naveen Kumar D, Rajkumar V, Suresh P, Ashok Kumar R . Quantitative assessment of the relative antineoplastic potential of the n-butanolic leaf extract of Annona muricata Linn. in normal and immortalized human cell lines. Asian Pac J Cancer Prev. 2012; 13(2):699-704. DOI: 10.7314/apjcp.2012.13.2.699. View

Bhattacharyya A, Chattopadhyay R, Mitra S, Crowe S . Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev. 2014; 94(2):329-54. PMC: 4044300. DOI: 10.1152/physrev.00040.2012. View

Albukhaty S, Naderi-Manesh H, Tiraihi T . In vitro labeling of neural stem cells with poly-L-lysine coated super paramagnetic nanoparticles for green fluorescent protein transfection. Iran Biomed J. 2013; 17(2):71-6. PMC: 3677676. DOI: 10.6091/ibj.1114.2013. View

Alyamani A, Albukhaty S, Aloufi S, AlMalki F, Al-Karagoly H, Sulaiman G . Green Fabrication of Zinc Oxide Nanoparticles Using Leaf Extract: Characterization and In Vitro Evaluation of Cytotoxicity and Antibacterial Properties. Molecules. 2021; 26(20). PMC: 8537537. DOI: 10.3390/molecules26206140. View

Hosseinzadeh N, Shomali T, Hosseinzadeh S, Raouf Fard F, Pourmontaseri M, Fazeli M . Green synthesis of gold nanoparticles by using Ferula persica Willd. gum essential oil: production, characterization and in vitro anti-cancer effects. J Pharm Pharmacol. 2020; 72(8):1013-1025. DOI: 10.1111/jphp.13274. View

Zivadinovic D, Watson C . Membrane estrogen receptor-alpha levels predict estrogen-induced ERK1/2 activation in MCF-7 cells. Breast Cancer Res. 2005; 7(1):R130-44. PMC: 1064105. DOI: 10.1186/bcr959. View

Nawwar M, Ayoub N, Hussein S, Hashim A, El-Sharawy R, Wende K . A flavonol triglycoside and investigation of the antioxidant and cell stimulating activities of Annona muricata Linn. Arch Pharm Res. 2012; 35(5):761-7. DOI: 10.1007/s12272-012-0501-4. View

Ovais M, Khalil A, Islam N, Ahmad I, Ayaz M, Saravanan M . Role of plant phytochemicals and microbial enzymes in biosynthesis of metallic nanoparticles. Appl Microbiol Biotechnol. 2018; 102(16):6799-6814. DOI: 10.1007/s00253-018-9146-7. View

10.

Hosseini-Koupaei M, Shareghi B, Saboury A, Davar F, Sirotkin V, Hosseini-Koupaei M . Catalytic activity, structure and stability of proteinase K in the presence of biosynthesized CuO nanoparticles. Int J Biol Macromol. 2018; 122:732-744. DOI: 10.1016/j.ijbiomac.2018.11.001. View

11.

Munoz-Escobar A, Ruiz-Baltazar A, Reyes-Lopez S . Novel Route of Synthesis of PCL-CuONPs Composites With Antimicrobial Properties. Dose Response. 2019; 17(3):1559325819869502. PMC: 6699009. DOI: 10.1177/1559325819869502. View

12.

Eng K, Panas M, Karlsson Hedestam G, McInerney G . A novel quantitative flow cytometry-based assay for autophagy. Autophagy. 2010; 6(5):634-41. DOI: 10.4161/auto.6.5.12112. View

13.

Naik A, Sellappan K . evaluation of L. (Soursop) leaf methanol extracts on inhibition of tumorigenicity and metastasis of breast cancer cells. Biomarkers. 2020; 25(8):701-710. DOI: 10.1080/1354750X.2020.1836025. View

14.

Esmaeili Govarchin Ghaleh H, Zarei L, Motlagh B, Jabbari N . Using CuO nanoparticles and hyperthermia in radiotherapy of MCF-7 cell line: synergistic effect in cancer therapy. Artif Cells Nanomed Biotechnol. 2019; 47(1):1396-1403. DOI: 10.1080/21691401.2019.1600529. View

15.

Duan H, Wang D, Li Y . Green chemistry for nanoparticle synthesis. Chem Soc Rev. 2015; 44(16):5778-92. DOI: 10.1039/c4cs00363b. View

16.

Nasrollahzadeh M, Sajadi S, Rostami-Vartooni A, Hussin S . Green synthesis of CuO nanoparticles using aqueous extract of Thymus vulgaris L. leaves and their catalytic performance for N-arylation of indoles and amines. J Colloid Interface Sci. 2015; 466:113-9. DOI: 10.1016/j.jcis.2015.12.018. View

17.

Al-Mohaimeed A, Mostafa G, El-Tohamy M . New Construction of Functionalized CuO/AlO Nanocomposite-Based Polymeric Sensor for Potentiometric Estimation of Naltrexone Hydrochloride in Commercial Formulations. Polymers (Basel). 2021; 13(24). PMC: 8703699. DOI: 10.3390/polym13244459. View

18.

Shafagh M, Rahmani F, Delirezh N . CuO nanoparticles induce cytotoxicity and apoptosis in human K562 cancer cell line via mitochondrial pathway, through reactive oxygen species and P53. Iran J Basic Med Sci. 2016; 18(10):993-1000. PMC: 4686584. View

19.

Chung I, Rahuman A, Marimuthu S, Kirthi A, Anbarasan K, Padmini P . Green synthesis of copper nanoparticles using leaves extract and their antioxidant and cytotoxic activities. Exp Ther Med. 2017; 14(1):18-24. PMC: 5488509. DOI: 10.3892/etm.2017.4466. View

20.

Albukhaty S, Al-Musawi S, Abdul Mahdi S, Sulaiman G, Alwahibi M, Dewir Y . Investigation of Dextran-Coated Superparamagnetic Nanoparticles for Targeted Vinblastine Controlled Release, Delivery, Apoptosis Induction, and Gene Expression in Pancreatic Cancer Cells. Molecules. 2020; 25(20). PMC: 7587551. DOI: 10.3390/molecules25204721. View