Association Analysis and Expression Level of ACE Polymorphisms with Egg-laying Trait in Taihang Chicken

Overview

Journal Poult Sci

Publisher Elsevier

Specialty Veterinary Medicine

Date 2022 Sep 26

PMID 36163094

Authors

Peng Wang

Kaiyang Li

Yekai Fan

Hui Zhang

Yifan Zhang

Ziyi Liu

Wentao Li

Haiyin Han

Yahui Gao

Jiannan Liu

Yufang Liu

Affiliations

Soon will be listed here.

Abstract

The number of egg-laying is an important indicator of reproduction performance in poultry breeding. To investigate the relationship between the function of Angiotensin-converting enzyme (ACE) and egg-laying performance of Taihang chicken, the mRNA and protein expression and single nucleotide polymorphism (SNP) of ACE were detected. Analysis of ACE bioinformatics and association analysis of polymorphisms were then performed. The polymorphisms analysis of ACE showed that three SNP loci (g.5066812A>C, g.5080076G>A, and g.5072728A>G) were detected in 800 Taihang chickens with egg-laying records. Association analysis of egg-laying found that ACE g.5066812A>C mutation was significantly associated with the egg-laying performance of Taihang chickens (P < 0.05), and the individuals with the g.5066812A>C mutation showed significantly increasing egg-laying. The mRNA expression was significantly higher in individuals with the AA genotype mutation than those with the AC and CC genotypes (P < 0.01), and the expression of ACE protein levels was consistent with the mRNA expression. Bioinformatics analysis indicated that these mutations affected the secondary and tertiary structure of ACE. This study provides new insights into ACE affecting chicken egg production and some basis for improving the egg production rate of Taihang chickens.

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