Comparative Physiological and Coexpression Network Analyses Reveal the Potential Drought Tolerance Mechanism of Peanut

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2022 Sep 26

PMID 36162997

Authors

Jingyao Ren

Pei Guo

He Zhang

Xiaolong Shi

Xin Ai

Jing Wang

Chunji Jiang

Xinhua Zhao

Xibo Liu

Haiqiu Yu

Affiliations

Soon will be listed here.

Abstract

Background: Drought stress has negative effects on plant growth and productivity. In this study, a comprehensive analysis of physiological responses and gene expression was performed. The responses and expressions were compared between drought-tolerant (DT) and drought-sensitive (DS) peanut varieties to investigate the regulatory mechanisms and hub genes involved in the impact of drought stress on culture.

Results: The drought-tolerant variety had robust antioxidative capacities with higher total antioxidant capacity and flavonoid contents, and it enhanced osmotic adjustment substance accumulation to adapt to drought conditions. KEGG analysis of differentially expressed genes demonstrated that photosynthesis was strongly affected by drought stress, especially in the drought-sensitive variety, which was consistent with the more severe suppression of photosynthesis. The hub genes in the key modules related to the drought response, including genes encoding protein kinase, E3 ubiquitin-protein ligase, potassium transporter, pentatricopeptide repeat-containing protein, and aspartic proteinase, were identified through a comprehensive combined analysis of genes and physiological traits using weighted gene co-expression network analysis. There were notably differentially expressed genes between the two varieties, suggesting the positive roles of these genes in peanut drought tolerance.

Conclusion: A comprehensive analysis of physiological traits and relevant genes was conducted on peanuts with different drought tolerances. The findings revealed diverse drought-response mechanisms and identified candidate genes for further research.

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References

Du J, Shen T, Xiong Q, Zhu C, Peng X, He X . Combined proteomics, metabolomics and physiological analyses of rice growth and grain yield with heavy nitrogen application before and after drought. BMC Plant Biol. 2020; 20(1):556. PMC: 7731554. DOI: 10.1186/s12870-020-02772-y. View

Chen W, Yao Q, Patil G, Agarwal G, Deshmukh R, Lin L . Identification and Comparative Analysis of Differential Gene Expression in Soybean Leaf Tissue under Drought and Flooding Stress Revealed by RNA-Seq. Front Plant Sci. 2016; 7:1044. PMC: 4950259. DOI: 10.3389/fpls.2016.01044. View

Lv L, Zhang W, Sun L, Zhao A, Zhang Y, Wang L . Gene co-expression network analysis to identify critical modules and candidate genes of drought-resistance in wheat. PLoS One. 2020; 15(8):e0236186. PMC: 7458298. DOI: 10.1371/journal.pone.0236186. View

Heath R, Packer L . Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys. 1968; 125(1):189-98. DOI: 10.1016/0003-9861(68)90654-1. View

Deng X, Liu Y, Xu X, Liu D, Zhu G, Yan X . Comparative Proteome Analysis of Wheat Flag Leaves and Developing Grains Under Water Deficit. Front Plant Sci. 2018; 9:425. PMC: 5902686. DOI: 10.3389/fpls.2018.00425. View

Gou X, He K, Yang H, Yuan T, Lin H, Clouse S . Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana. BMC Genomics. 2010; 11:19. PMC: 2817689. DOI: 10.1186/1471-2164-11-19. View

Guo C, Xie Y, Zhu M, Xiong Q, Chen Y, Yu Q . Influence of different cooking methods on the nutritional and potentially harmful components of peanuts. Food Chem. 2020; 316:126269. DOI: 10.1016/j.foodchem.2020.126269. View

Feng X, Liu W, Cao F, Wang Y, Zhang G, Chen Z . Overexpression of HvAKT1 improves drought tolerance in barley by regulating root ion homeostasis and ROS and NO signaling. J Exp Bot. 2020; 71(20):6587-6600. DOI: 10.1093/jxb/eraa354. View

Leshem Y, Golani Y, Kaye Y, Levine A . Reduced expression of the v-SNAREs AtVAMP71/AtVAMP7C gene family in Arabidopsis reduces drought tolerance by suppression of abscisic acid-dependent stomatal closure. J Exp Bot. 2010; 61(10):2615-22. PMC: 2882261. DOI: 10.1093/jxb/erq099. View

10.

Velazquez-Marquez S, Conde-Martinez V, Trejo C, Delgado-Alvarado A, Carballo A, Suarez R . Effects of water deficit on radicle apex elongation and solute accumulation in Zea mays L. Plant Physiol Biochem. 2015; 96:29-37. DOI: 10.1016/j.plaphy.2015.07.006. View

11.

Leng G, Hall J . Crop yield sensitivity of global major agricultural countries to droughts and the projected changes in the future. Sci Total Environ. 2018; 654:811-821. PMC: 6341212. DOI: 10.1016/j.scitotenv.2018.10.434. View

12.

Sebastian D, Fernando F, Raul D, Gabriela G . Overexpression of Arabidopsis aspartic protease APA1 gene confers drought tolerance. Plant Sci. 2020; 292:110406. DOI: 10.1016/j.plantsci.2020.110406. View

13.

Luo Y, Pang D, Jin M, Chen J, Kong X, Li W . Identification of plant hormones and candidate hub genes regulating flag leaf senescence in wheat response to water deficit stress at the grain-filling stage. Plant Direct. 2019; 3(11):e00152. PMC: 6834085. DOI: 10.1002/pld3.152. View

14.

Choe J, Kim G, Kim H . Effects of green tea leaf, lotus leaf, and kimchi powders on quality characteristics of chicken liver sausages. J Anim Sci Technol. 2019; 61(1):28-34. PMC: 6582918. DOI: 10.5187/jast.2019.61.1.28. View

15.

Yang Q, Cheng L, Long Z, Li H, Gunaratne A, Gan R . Comparison of the Phenolic Profiles of Soaked and Germinated Peanut Cultivars via UPLC-QTOF-MS. Antioxidants (Basel). 2019; 8(2). PMC: 6406428. DOI: 10.3390/antiox8020047. View

16.

Wang M, Zheng Q, Shen Q, Guo S . The critical role of potassium in plant stress response. Int J Mol Sci. 2013; 14(4):7370-90. PMC: 3645691. DOI: 10.3390/ijms14047370. View

17.

Tang X, Ghimire S, Liu W, Fu X, Zhang H, Zhang N . Potato E3 ubiquitin ligase PUB27 negatively regulates drought tolerance by mediating stomatal movement. Plant Physiol Biochem. 2020; 154:557-563. DOI: 10.1016/j.plaphy.2020.07.026. View

18.

Deikman J, Petracek M, Heard J . Drought tolerance through biotechnology: improving translation from the laboratory to farmers' fields. Curr Opin Biotechnol. 2011; 23(2):243-50. DOI: 10.1016/j.copbio.2011.11.003. View

19.

Zhu M, Xie H, Wei X, Dossa K, Yu Y, Hui S . WGCNA Analysis of Salt-Responsive Core Transcriptome Identifies Novel Hub Genes in Rice. Genes (Basel). 2019; 10(9). PMC: 6771013. DOI: 10.3390/genes10090719. View

20.

Ahumada-Fierro N, Garcia-Mendoza E, Sandoval-Gil J, Band-Schmidt C . Photosynthesis and photoprotection characteristics related to ROS production in three Chattonella (Raphidophyceae) species. J Phycol. 2021; 57(3):941-954. DOI: 10.1111/jpy.13138. View