Physicochemical Characterization of a Polysaccharide from Tratt Fruit and Its Antitumor Activity by Activating ROS Mediated Pathways

Overview

Journal Curr Res Food Sci

Date 2022 Sep 26

PMID 36161228

Authors

Yanlin Jin

YingHua Li

Lei Wang

Xiong Fu

Chao Li

Affiliations

Soon will be listed here.

Abstract

Tratt fruit is a highly valued fruit that contains abundant functional and nutritional constituents. In this study, a novel polysaccharide, named RTFP-1, was isolated and purified from Tratt fruit. Structural characterization indicated that RTFP-1 was a homogeneous heteropolysaccharide with the molecular weight (Mw) of 128.7 kDa and consisted of arabinose, galactose, glucose, mannose, xylose, and fucose with molar ratio percentages of 34.84, 40.59, 12.11, 5.06, 3.39, and 4.01%, respectively. A CCK-8 assay indicated that RTFP-1 inhibited the cell growth of HepG2 cells in a dose-dependent manner. Morphological analysis and flow cytometry experiment showed that RTFP-1 promoted the apoptosis of HepG2 cells and increased reactive oxygen species (ROS) level. The underlying molecular mechanisms indicated that RTFP-1 activated the apoptosis of HepG2 cells through ROS-mediated MAPK, STAT, and p53 apoptotic pathways. These results suggest that RTFP-1 might be a potential chemopreventive and antitumor agent.

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References

Yang H, Villani R, Wang H, Simpson M, Roberts M, Tang M . The role of cellular reactive oxygen species in cancer chemotherapy. J Exp Clin Cancer Res. 2018; 37(1):266. PMC: 6211502. DOI: 10.1186/s13046-018-0909-x. View

Carneiro B, El-Deiry W . Targeting apoptosis in cancer therapy. Nat Rev Clin Oncol. 2020; 17(7):395-417. PMC: 8211386. DOI: 10.1038/s41571-020-0341-y. View

Zhang Y, Zhou T, Wang H, Cui Z, Cheng F, Wang K . Structural characterization and in vitro antitumor activity of an acidic polysaccharide from Angelica sinensis (Oliv.) Diels. Carbohydr Polym. 2016; 147:401-408. DOI: 10.1016/j.carbpol.2016.04.002. View

Madduru D, Ijaq J, Dhar S, Sarkar S, Poondla N, Das P . Systems Challenges of Hepatic Carcinomas: A Review. J Clin Exp Hepatol. 2019; 9(2):233-244. PMC: 6477144. DOI: 10.1016/j.jceh.2018.05.002. View

Sung H, Ferlay J, Siegel R, Laversanne M, Soerjomataram I, Jemal A . Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 2021; 71(3):209-249. DOI: 10.3322/caac.21660. View

McGlynn K, Petrick J, El-Serag H . Epidemiology of Hepatocellular Carcinoma. Hepatology. 2020; 73 Suppl 1:4-13. PMC: 7577946. DOI: 10.1002/hep.31288. View

Verhoeven Y, Tilborghs S, Jacobs J, De Waele J, Quatannens D, Deben C . The potential and controversy of targeting STAT family members in cancer. Semin Cancer Biol. 2019; 60:41-56. DOI: 10.1016/j.semcancer.2019.10.002. View

Chen Y, Liu Z, Liu J, Liu L, Zhang E, Li W . Inhibition of metastasis and invasion of ovarian cancer cells by crude polysaccharides from rosa roxburghii tratt in vitro. Asian Pac J Cancer Prev. 2015; 15(23):10351-4. DOI: 10.7314/apjcp.2014.15.23.10351. View

Zhang M, Tang X, Wang F, Zhang Q, Zhang Z . Characterization of Lycium barbarum polysaccharide and its effect on human hepatoma cells. Int J Biol Macromol. 2013; 61:270-5. DOI: 10.1016/j.ijbiomac.2013.06.031. View

10.

Lee S, Rauch J, Kolch W . Targeting MAPK Signaling in Cancer: Mechanisms of Drug Resistance and Sensitivity. Int J Mol Sci. 2020; 21(3). PMC: 7037308. DOI: 10.3390/ijms21031102. View

11.

Xia Y, Xiao M, Zhao M, Xu T, Guo M, Wang C . Doxorubicin-loaded functionalized selenium nanoparticles for enhanced antitumor efficacy in cervical carcinoma therapy. Mater Sci Eng C Mater Biol Appl. 2019; 106:110100. DOI: 10.1016/j.msec.2019.110100. View

12.

Li C, Dong Z, Zhang B, Huang Q, Liu G, Fu X . Structural characterization and immune enhancement activity of a novel polysaccharide from Moringa oleifera leaves. Carbohydr Polym. 2020; 234:115897. DOI: 10.1016/j.carbpol.2020.115897. View

13.

Anwanwan D, Singh S, Singh S, Saikam V, Singh R . Challenges in liver cancer and possible treatment approaches. Biochim Biophys Acta Rev Cancer. 2019; 1873(1):188314. PMC: 6981221. DOI: 10.1016/j.bbcan.2019.188314. View

14.

Wang L, Chen C, Zhang B, Huang Q, Fu X, Li C . Structural characterization of a novel acidic polysaccharide from Rosa roxburghii Tratt fruit and its α-glucosidase inhibitory activity. Food Funct. 2018; 9(7):3974-3985. DOI: 10.1039/c8fo00561c. View

15.

Su J, Fu X, Huang Q, Liu G, Li C . Phytochemical profile, bioactivity and prebiotic potential of bound polyphenols released from fruit pomace dietary fiber during digestion and fermentation. Food Funct. 2022; 13(17):8880-8891. DOI: 10.1039/d2fo00823h. View

16.

Ray P, Huang B, Tsuji Y . Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell Signal. 2012; 24(5):981-90. PMC: 3454471. DOI: 10.1016/j.cellsig.2012.01.008. View

17.

Wang L, Li C, Huang Q, Fu X . Polysaccharide from Tratt Fruit Attenuates Hyperglycemia and Hyperlipidemia and Regulates Colon Microbiota in Diabetic Mice. J Agric Food Chem. 2019; 68(1):147-159. DOI: 10.1021/acs.jafc.9b06247. View

18.

Van Opdenbosch N, Lamkanfi M . Caspases in Cell Death, Inflammation, and Disease. Immunity. 2019; 50(6):1352-1364. PMC: 6611727. DOI: 10.1016/j.immuni.2019.05.020. View

19.

Li Y, Guo M, Lin Z, Zhao M, Xia Y, Wang C . Multifunctional selenium nanoparticles with Galangin-induced HepG2 cell apoptosis through p38 and AKT signalling pathway. R Soc Open Sci. 2018; 5(11):180509. PMC: 6281927. DOI: 10.1098/rsos.180509. View

20.

Zhang K, Zhou X, Wang J, Zhou Y, Qi W, Chen H . Dendrobium officinale polysaccharide triggers mitochondrial disorder to induce colon cancer cell death via ROS-AMPK-autophagy pathway. Carbohydr Polym. 2021; 264:118018. DOI: 10.1016/j.carbpol.2021.118018. View