High-resolution Mapping Reveals a -like Locus Against Northern Corn Leaf Blight

Overview

Journal Front Plant Sci

Date 2022 Sep 26

PMID 36160951

Authors

Mang Zhu

Jun Ma

Xinfang Liu

Yanling Guo

Xin Qi

Xue Gong

Yanbin Zhu

Yanbo Wang

Min Jiang

Affiliations

Soon will be listed here.

Abstract

Northern corn leaf blight (NCLB), caused by the fungal pathogen , poses a grave threat to maize production worldwide. The resistance gene in A619, discovered decades ago, is an important genetic resource for NCLB control. By using a pair of near-isogenic lines (NILs) A619 and A619, together with the resistant and susceptible bulks derived from the cross of A619 and L3162 lines, we initially detected a -like () locus in bin 8.06 that was closely associated with NCLB resistance. We then performed five rounds of fine-mapping, which ultimately delimited the locus to a 577-kb interval flanked by SNP markers KA002081 and KA002084. Plants homozygous for the / genotype exhibited an average reduction in diseased leaf area (DLA) by 16.5% compared to plants lacking . The locus showed extensive variation in genomic architecture among different maize lines and did not appear to contain any genes encoding canonical cell wall-associated kinases against NCLB. Moreover, the locus was located ∼2.7 Mb away from the known locus. We speculate that the locus may contain a bona fide gene or a novel NCLB resistance gene closely linked to . In practice, the locus is a valuable resource for improving maize resistance to NCLB.

Citing Articles

The ZmCPK39-ZmDi19-ZmPR10 immune module regulates quantitative resistance to multiple foliar diseases in maize.

Zhu M, Zhong T, Xu L, Guo C, Zhang X, Liu Y Nat Genet. 2024; 56(12):2815-2826.

PMID: 39496881 PMC: 11631770. DOI: 10.1038/s41588-024-01968-4.

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