Germline Transformation of L. Plant Via Transformation Technology "Floral Dip"

Overview

Journal Biotechnol Rep (Amst)

Specialty Biotechnology

Date 2022 Sep 26

PMID 36159743

Authors

Firdaus Qamar

Shazia Khan

Kudsiya Ashrafi

Sadia Iqrar

Syed Naved Quadri

Monica Saifi

M Z Abdin

Affiliations

Soon will be listed here.

Abstract

The therapeutic efficacy of L. is governed by artemisinin (ART), prevalently produced by extraction. Due to the modest amount of ART (0.01-1 %dw) in this plant, commercialization of ACTs is difficult. In this study, the floral-dip based transformation protocol for was developed to enhance expression of artemisinin biosynthesis genes and ART content. For dipping, the effective infiltration media components were optimized, and to obtain high transformation (26.9%) partially open bud stage capitulum of floral development was used. Hygromycin phospho-transferase () selection marker was used to validate the transformed T progenies. The copy numbers of the transgene () in T progenies were determined using a sensitive, high-throughput SYBR Green based quantitative RT-PCR. The results of the transgene were compared with those of the low copy number, internal standard (). Using optimised PCR conditions, one, two and three transgene copies in T transformants were achieved.

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