Coordinate Regulation of Methanol Utilization Pathway Genes of by Transcription Factors and Chromatin Modifiers

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2022 Sep 23

PMID 36147846

Authors

Aditi Gupta

Pundi N Rangarajan

Affiliations

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Abstract

The methylotrophic yeast () harbors a methanol utilization (MUT) pathway, enabling it to utilize methanol as the sole source of carbon. The nexus between transcription factors such as Mxr1p and Trm1p and chromatin-modifying enzymes in the regulation of genes of MUT pathway has not been well studied in . Using transcriptomics, we demonstrate that Gcn5, a histone acetyltransferase, and Gal83, one of the beta subunits of nuclear-localized SNF1 (sucrose non-fermenting 1) kinase complex are essential for the transcriptional regulation by the zinc finger transcription factors Mxr1p and Trm1p. We conclude that interactions among Gcn5, Snf1, Mxr1p, and Trm1p play a critical role in the transcriptional regulation of genes of MUT pathway of .

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