Application of Optical Tweezers in Cardiovascular Research: More Than Just a Measuring Tool

Overview

Journal Front Bioeng Biotechnol

Date 2022 Sep 23

PMID 36147537

Authors

Yi Yang

Zhenhai Fu

Wei Zhu

Huizhu Hu

Jianan Wang

Affiliations

Soon will be listed here.

Abstract

Recent advances in the field of optical tweezer technology have shown intriguing potential for applications in cardiovascular medicine, bringing this laboratory nanomechanical instrument into the spotlight of translational medicine. This article summarizes cardiovascular system findings generated using optical tweezers, including not only rigorous nanomechanical measurements but also multifunctional manipulation of biologically active molecules such as myosin and actin, of cells such as red blood cells and cardiomyocytes, of subcellular organelles, and of microvessels . The implications of these findings in the diagnosis and treatment of diseases, as well as potential perspectives that could also benefit from this tool, are also discussed.

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References

Tyska M, Hayes E, Giewat M, Seidman C, Seidman J, Warshaw D . Single-molecule mechanics of R403Q cardiac myosin isolated from the mouse model of familial hypertrophic cardiomyopathy. Circ Res. 2000; 86(7):737-44. DOI: 10.1161/01.res.86.7.737. View

Nanasi P, Komaromi I, Gaburjakova M, Almassy J . Omecamtiv Mecarbil: A Myosin Motor Activator Agent with Promising Clinical Performance and New in vitro Results. Curr Med Chem. 2017; 25(15):1720-1728. DOI: 10.2174/0929867325666171222164320. View

Kwon W, Song Y . Diabetes Mellitus With Coronary Artery Disease: Identifying Subgroups in Need of Additional Monitoring. JACC Asia. 2022; 2(3):309-310. PMC: 9627823. DOI: 10.1016/j.jacasi.2022.02.006. View

Biering-Sorensen T, Minamisawa M, Claggett B, Liu J, Felker G, McMurray J . Cardiac Myosin Activator Omecamtiv Mecarbil Improves Left Ventricular Myocardial Deformation in Chronic Heart Failure: The COSMIC-HF Trial. Circ Heart Fail. 2020; 13(12):e008007. DOI: 10.1161/CIRCHEARTFAILURE.120.008007. View

Chan J, Motton D, Rutledge J, Keim N, Huser T . Raman spectroscopic analysis of biochemical changes in individual triglyceride-rich lipoproteins in the pre- and postprandial state. Anal Chem. 2005; 77(18):5870-6. DOI: 10.1021/ac050692f. View

Feller T, Connell S, Arijons R . Why fibrin biomechanical properties matter for hemostasis and thrombosis. J Thromb Haemost. 2021; 20(1):6-16. DOI: 10.1111/jth.15531. View

Bianco P, Nagy A, Kengyel A, Szatmari D, Martonfalvi Z, Huber T . Interaction forces between F-actin and titin PEVK domain measured with optical tweezers. Biophys J. 2007; 93(6):2102-9. PMC: 1959548. DOI: 10.1529/biophysj.107.106153. View

Gentemann L, Kalies S, Coffee M, Meyer H, Ripken T, Heisterkamp A . Modulation of cardiomyocyte activity using pulsed laser irradiated gold nanoparticles. Biomed Opt Express. 2017; 8(1):177-192. PMC: 5231291. DOI: 10.1364/BOE.8.000177. View

Jagiello A, Castillo U, Botvinick E . Cell mediated remodeling of stiffness matched collagen and fibrin scaffolds. Sci Rep. 2022; 12(1):11736. PMC: 9273755. DOI: 10.1038/s41598-022-14953-w. View

10.

McCully J, Levitsky S, Del Nido P, Cowan D . Mitochondrial transplantation for therapeutic use. Clin Transl Med. 2016; 5(1):16. PMC: 4851669. DOI: 10.1186/s40169-016-0095-4. View

11.

Jordan P, Leach J, Padgett M, Blackburn P, Isaacs N, Goksor M . Creating permanent 3D arrangements of isolated cells using holographic optical tweezers. Lab Chip. 2005; 5(11):1224-8. DOI: 10.1039/b509218c. View

12.

Anand S, Nylk J, Neale S, Dodds C, Grant S, Ismail M . Aerosol droplet optical trap loading using surface acoustic wave nebulization. Opt Express. 2014; 21(25):30148-55. DOI: 10.1364/OE.21.030148. View

13.

Hook P, Litvinov R, Kim O, Xu S, Xu Z, Bennett J . Strong Binding of Platelet Integrin αIIbβ3 to Fibrin Clots: Potential Target to Destabilize Thrombi. Sci Rep. 2017; 7(1):13001. PMC: 5636895. DOI: 10.1038/s41598-017-12615-w. View

14.

Tsurupa G, Pechik I, Litvinov R, Hantgan R, Tjandra N, Weisel J . On the mechanism of αC polymer formation in fibrin. Biochemistry. 2012; 51(12):2526-38. PMC: 3343699. DOI: 10.1021/bi2017848. View

15.

Koirala D, Yu Z, Dhakal S, Mao H . Detection of single nucleotide polymorphism using tension-dependent stochastic behavior of a single-molecule template. J Am Chem Soc. 2011; 133(26):9988-91. DOI: 10.1021/ja201976r. View

16.

Ishii S, Kawai M, Ishiwata S, Suzuki M . Estimation of actomyosin active force maintained by tropomyosin and troponin complex under vertical forces in the in vitro motility assay system. PLoS One. 2018; 13(2):e0192558. PMC: 5805308. DOI: 10.1371/journal.pone.0192558. View

17.

Shin B, Saeed M, Esch J, Guariento A, Blitzer D, Moskowitzova K . A Novel Biological Strategy for Myocardial Protection by Intracoronary Delivery of Mitochondria: Safety and Efficacy. JACC Basic Transl Sci. 2020; 4(8):871-888. PMC: 6938990. DOI: 10.1016/j.jacbts.2019.08.007. View

18.

Tskhovrebova L, Trinick J, Sleep J, Simmons R . Elasticity and unfolding of single molecules of the giant muscle protein titin. Nature. 1997; 387(6630):308-12. DOI: 10.1038/387308a0. View

19.

Collet J, Shuman H, Ledger R, Lee S, Weisel J . The elasticity of an individual fibrin fiber in a clot. Proc Natl Acad Sci U S A. 2005; 102(26):9133-7. PMC: 1166643. DOI: 10.1073/pnas.0504120102. View

20.

Martonfalvi Z, Bianco P, Linari M, Caremani M, Nagy A, Lombardi V . Low-force transitions in single titin molecules reflect a memory of contractile history. J Cell Sci. 2013; 127(Pt 4):858-70. DOI: 10.1242/jcs.138461. View