Transcriptional Regulation Mechanism of Flavonoids Biosynthesis Gene During Fruit Development in

Overview

Journal Front Genet

Date 2022 Sep 23

PMID 36147500

Authors

Pengfei Hu

Suriguga

Ming Zhao

Shaoqing Chen

Xiaohua Wu

Quan Wan

Affiliations

Soon will be listed here.

Abstract

, as an important medicinal plant, are an excellent source of flavonoids. Flavonoid compounds in have been widely used in medicine and supplement, but known of the molecular mechanism of flavonoid biosynthesis is still very few. Here, we analyzed the association between flavonoid content and gene expression pattern during six different fruit developmental stages. Sixteen gene expression trends were significantly identified, involving 8,218 genes. The gene expression trend in profile 0 was positively correlated with flavonoid content, while the gene expression trend in profile 79 was negatively correlated with flavonoid content at six developmental stages. The expression level of genes involved in the general phenylpropane pathway was higher than that of genes involved in the flavonoid biosynthesis pathway. A total of 37 genes involved in flavonoid synthesis were identified in . The expression pattern of flavonoid-related genes was highly correlated with flavonoid content. Our study deepened the understanding of the flavonoid synthesis mechanism and provided useful resources for future studies on the high flavonoid molecular breeding of .

Citing Articles

Germplasm Resources and Genetic Breeding of Huang-Qi (Astragali Radix): A Systematic Review.

Dong P, Wang L, Chen Y, Wang L, Liang W, Wang H Biology (Basel). 2024; 13(8).

PMID: 39194563 PMC: 11351161. DOI: 10.3390/biology13080625.

Pharmacological effects of Astragalus polysaccharides in treating neurodegenerative diseases.

Shi Y, Ma P Front Pharmacol. 2024; 15:1449101.

PMID: 39156112 PMC: 11327089. DOI: 10.3389/fphar.2024.1449101.

: A Review of Its Antitumor Effects on Non-Small Cell Lung Cancer.

Li Z, Liu J, Cui H, Qi W, Tong Y, Wang T Cancer Manag Res. 2024; 16:909-919.

PMID: 39081698 PMC: 11287463. DOI: 10.2147/CMAR.S466633.

Combined Metabolomics and Transcriptomics Analysis of the Distribution of Flavonoids in the Fibrous Root and Taproot of Coll.et Hemsl.

Mo X, Wang L, Yu C, Kou C Genes (Basel). 2024; 15(7).

PMID: 39062607 PMC: 11275391. DOI: 10.3390/genes15070828.

Integrated Transcriptomics and Metabolomics Analysis Reveals the Effects of Cutting on the Synthesis of Flavonoids and Saponins in Chinese Herbal Medicine .

Guo X, Yan X, Wang Y, Shi Z, Niu J, Liang J Metabolites. 2024; 14(2).

PMID: 38392989 PMC: 10891646. DOI: 10.3390/metabo14020097.

References

Martens S, Forkmann G, Britsch L, Wellmann F, Matern U, Lukacin R . Divergent evolution of flavonoid 2-oxoglutarate-dependent dioxygenases in parsley. FEBS Lett. 2003; 544(1-3):93-8. DOI: 10.1016/s0014-5793(03)00479-4. View

Auyeung K, Han Q, Ko J . Astragalus membranaceus: A Review of its Protection Against Inflammation and Gastrointestinal Cancers. Am J Chin Med. 2016; 44(1):1-22. DOI: 10.1142/S0192415X16500014. View

Liu P, Zhao H, Luo Y . Anti-Aging Implications of (Huangqi): A Well-Known Chinese Tonic. Aging Dis. 2018; 8(6):868-886. PMC: 5758356. DOI: 10.14336/AD.2017.0816. View

Zhang Y, Gu L, Xia Q, Tian L, Qi J, Cao M . Radix Astragali and Radix Angelicae Sinensis in the Treatment of Idiopathic Pulmonary Fibrosis: A Systematic Review and Meta-analysis. Front Pharmacol. 2020; 11:415. PMC: 7203419. DOI: 10.3389/fphar.2020.00415. View

Shen N, Wang T, Gan Q, Liu S, Wang L, Jin B . Plant flavonoids: Classification, distribution, biosynthesis, and antioxidant activity. Food Chem. 2022; 383:132531. DOI: 10.1016/j.foodchem.2022.132531. View

Santin O, Moncalian G . Loading of malonyl-CoA onto tandem acyl carrier protein domains of polyunsaturated fatty acid synthases. J Biol Chem. 2018; 293(32):12491-12501. PMC: 6093242. DOI: 10.1074/jbc.RA118.002443. View

Wang S, Yang C, Tu H, Zhou J, Liu X, Cheng Y . Characterization and Metabolic Diversity of Flavonoids in Citrus Species. Sci Rep. 2017; 7(1):10549. PMC: 5585201. DOI: 10.1038/s41598-017-10970-2. View

Jiang L, Schlesinger F, Davis C, Zhang Y, Li R, Salit M . Synthetic spike-in standards for RNA-seq experiments. Genome Res. 2011; 21(9):1543-51. PMC: 3166838. DOI: 10.1101/gr.121095.111. View

Song J, Zhou Y, Pang Y, Gao L, Du G, Qin X . The anti-aging effect of Scutellaria baicalensis Georgi flowers extract by regulating the glutamine-glutamate metabolic pathway in d-galactose induced aging rats. Exp Gerontol. 2020; 134:110843. DOI: 10.1016/j.exger.2020.110843. View

10.

Doring K, Langeder J, Duwe S, Tahir A, Grienke U, Rollinger J . Insights into the direct anti-influenza virus mode of action of Rhodiola rosea. Phytomedicine. 2022; 96:153895. DOI: 10.1016/j.phymed.2021.153895. View

11.

Zhao C, Liu X, Gong Q, Cao J, Shen W, Yin X . Three AP2/ERF family members modulate flavonoid synthesis by regulating type IV chalcone isomerase in citrus. Plant Biotechnol J. 2020; 19(4):671-688. PMC: 8051604. DOI: 10.1111/pbi.13494. View

12.

Anders S, Pyl P, Huber W . HTSeq--a Python framework to work with high-throughput sequencing data. Bioinformatics. 2014; 31(2):166-9. PMC: 4287950. DOI: 10.1093/bioinformatics/btu638. View

13.

Song J, Mo S, Yip Y, Qiao C, Han Q, Xu H . Development of microwave assisted extraction for the simultaneous determination of isoflavonoids and saponins in radix astragali by high performance liquid chromatography. J Sep Sci. 2007; 30(6):819-24. DOI: 10.1002/jssc.200600340. View

14.

Espley R, Leif D, Plunkett B, McGhie T, Henry-Kirk R, Hall M . Red to Brown: An Elevated Anthocyanic Response in Apple Drives Ethylene to Advance Maturity and Fruit Flesh Browning. Front Plant Sci. 2019; 10:1248. PMC: 6794385. DOI: 10.3389/fpls.2019.01248. View

15.

Lukacin R, Matern U, Junghanns K, Heskamp M, Britsch L, Forkmann G . Purification and antigenicity of flavone synthase I from irradiated parsley cells. Arch Biochem Biophys. 2001; 393(1):177-83. DOI: 10.1006/abbi.2001.2491. View

16.

Chen T, Zhang H, Liu Y, Liu Y, Huang L . EVenn: Easy to create repeatable and editable Venn diagrams and Venn networks online. J Genet Genomics. 2021; 48(9):863-866. DOI: 10.1016/j.jgg.2021.07.007. View

17.

Koirala N, Thuan N, Ghimire G, Van Thang D, Sohng J . Methylation of flavonoids: Chemical structures, bioactivities, progress and perspectives for biotechnological production. Enzyme Microb Technol. 2016; 86:103-16. DOI: 10.1016/j.enzmictec.2016.02.003. View

18.

Singla R, Dubey A, Garg A, Sharma R, Fiorino M, Ameen S . Natural Polyphenols: Chemical Classification, Definition of Classes, Subcategories, and Structures. J AOAC Int. 2019; 102(5):1397-1400. DOI: 10.5740/jaoacint.19-0133. View

19.

Wang L, Cui J, Jin B, Zhao J, Xu H, Lu Z . Multifeature analyses of vascular cambial cells reveal longevity mechanisms in old trees. Proc Natl Acad Sci U S A. 2020; 117(4):2201-2210. PMC: 6995005. DOI: 10.1073/pnas.1916548117. View

20.

Wang C, Dong Y, Zhu L, Wang L, Yan L, Wang M . Comparative transcriptome analysis of two contrasting wolfberry genotypes during fruit development and ripening and characterization of the LrMYB1 transcription factor that regulates flavonoid biosynthesis. BMC Genomics. 2020; 21(1):295. PMC: 7147035. DOI: 10.1186/s12864-020-6663-4. View