Amphiphysin AoRvs167-Mediated Membrane Curvature Facilitates Trap Formation, Endocytosis, and Stress Resistance in

Overview

Journal Pathogens

Date 2022 Sep 23

PMID 36145429

Authors

Peijie Cui

Mengqing Tian

Jinrong Huang

Xi Zheng

Yingqi Guo

Guohong Li

Xin Wang

Affiliations

Soon will be listed here.

Abstract

Bin1/Amphiphysin/Rvs (BAR) domain-containing proteins mediate fundamental cellular processes, including membrane remodeling and endocytosis. Nematode-trapping (NT) fungi can differentiate to form trapping structures through highly reorganized cell membranes and walls. In this study, we identified the NT fungus ortholog of yeast Rvs167 and documented its involvement in membrane bending and endocytosis. We further confirmed that the deletion of makes the fungus more hypersensitive to osmotic salt (Nacl), higher temperatures (28 to 30 °C), and the cell wall perturbation agent Congo red. In addition, the disruption of reduced the trap formation capacity. Hence, may regulate fungal pathogenicity through the integrity of plasma membranes and cell walls.

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References

Gkourtsa A, van den Burg J, Strijbis K, Avula T, Bijvoets S, Timm D . Identification and characterization of Rvs162/Rvs167-3, a novel N-BAR heterodimer in the human fungal pathogen Candida albicans. Eukaryot Cell. 2014; 14(2):182-93. PMC: 4311921. DOI: 10.1128/EC.00282-14. View

Bernardino de la Serna J, Schutz G, Eggeling C, Cebecauer M . There Is No Simple Model of the Plasma Membrane Organization. Front Cell Dev Biol. 2016; 4:106. PMC: 5040727. DOI: 10.3389/fcell.2016.00106. View

Kornitzer D . Regulation of Hyphal Morphogenesis by Endogenous Signals. J Fungi (Basel). 2019; 5(1). PMC: 6463138. DOI: 10.3390/jof5010021. View

Wan J, Dai Z, Zhang K, Li G, Zhao P . Pathogenicity and Metabolites of Endoparasitic Nematophagous Fungus YMF1.01759 against Nematodes. Microorganisms. 2021; 9(8). PMC: 8401723. DOI: 10.3390/microorganisms9081735. View

Ren G, Vajjhala P, Lee J, Winsor B, Munn A . The BAR domain proteins: molding membranes in fission, fusion, and phagy. Microbiol Mol Biol Rev. 2006; 70(1):37-120. PMC: 1393252. DOI: 10.1128/MMBR.70.1.37-120.2006. View

Zhou D, Zhu Y, Bai N, Yang L, Xie M, Yang J . AoATG5 plays pleiotropic roles in vegetative growth, cell nucleus development, conidiation, and virulence in the nematode-trapping fungus Arthrobotrys oligospora. Sci China Life Sci. 2021; 65(2):412-425. DOI: 10.1007/s11427-020-1913-9. View

Zhen Z, Zhang G, Yang L, Ma N, Li Q, Ma Y . Characterization and functional analysis of calcium/calmodulin-dependent protein kinases (CaMKs) in the nematode-trapping fungus Arthrobotrys oligospora. Appl Microbiol Biotechnol. 2018; 103(2):819-832. DOI: 10.1007/s00253-018-9504-5. View

Yang X, Ma N, Yang L, Zheng Y, Zhen Z, Li Q . Two Rab GTPases play different roles in conidiation, trap formation, stress resistance, and virulence in the nematode-trapping fungus Arthrobotrys oligospora. Appl Microbiol Biotechnol. 2018; 102(10):4601-4613. DOI: 10.1007/s00253-018-8929-1. View

Zhen Z, Xing X, Xie M, Yang L, Yang X, Zheng Y . MAP kinase Slt2 orthologs play similar roles in conidiation, trap formation, and pathogenicity in two nematode-trapping fungi. Fungal Genet Biol. 2018; 116:42-50. DOI: 10.1016/j.fgb.2018.04.011. View

10.

Mim C, Unger V . Membrane curvature and its generation by BAR proteins. Trends Biochem Sci. 2012; 37(12):526-33. PMC: 3508348. DOI: 10.1016/j.tibs.2012.09.001. View

11.

De Rossi P, Nomura T, Andrew R, Masse N, Sampathkumar V, Musial T . Neuronal BIN1 Regulates Presynaptic Neurotransmitter Release and Memory Consolidation. Cell Rep. 2020; 30(10):3520-3535.e7. PMC: 7146643. DOI: 10.1016/j.celrep.2020.02.026. View

12.

Su H, Zhao Y, Zhou J, Feng H, Jiang D, Zhang K . Trapping devices of nematode-trapping fungi: formation, evolution, and genomic perspectives. Biol Rev Camb Philos Soc. 2015; 92(1):357-368. DOI: 10.1111/brv.12233. View

13.

Toume M, Tani M . Yeast lacking the amphiphysin family protein Rvs167 is sensitive to disruptions in sphingolipid levels. FEBS J. 2016; 283(15):2911-28. DOI: 10.1111/febs.13783. View

14.

van der Spoel D, Lindahl E, Hess B, Groenhof G, Mark A, Berendsen H . GROMACS: fast, flexible, and free. J Comput Chem. 2005; 26(16):1701-18. DOI: 10.1002/jcc.20291. View

15.

Vajda S, Yueh C, Beglov D, Bohnuud T, Mottarella S, Xia B . New additions to the ClusPro server motivated by CAPRI. Proteins. 2016; 85(3):435-444. PMC: 5313348. DOI: 10.1002/prot.25219. View

16.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

17.

Myers M, Ryazantsev S, Hicke L, Payne G . Calmodulin Promotes N-BAR Domain-Mediated Membrane Constriction and Endocytosis. Dev Cell. 2016; 37(2):162-73. PMC: 4855880. DOI: 10.1016/j.devcel.2016.03.012. View

18.

Stamenova S, Dunn R, Adler A, Hicke L . The Rsp5 ubiquitin ligase binds to and ubiquitinates members of the yeast CIN85-endophilin complex, Sla1-Rvs167. J Biol Chem. 2004; 279(16):16017-25. DOI: 10.1074/jbc.M313479200. View

19.

Kozakov D, Beglov D, Bohnuud T, Mottarella S, Xia B, Hall D . How good is automated protein docking?. Proteins. 2013; 81(12):2159-66. PMC: 3934018. DOI: 10.1002/prot.24403. View

20.

Feyder S, De Craene J, Bar S, Bertazzi D, Friant S . Membrane trafficking in the yeast Saccharomyces cerevisiae model. Int J Mol Sci. 2015; 16(1):1509-25. PMC: 4307317. DOI: 10.3390/ijms16011509. View