Antigenic Characterization of Neuraminidase of Influenza A/H7N9 Viruses Isolated in Different Years

Overview

Journal Pharmaceuticals (Basel)

Publisher MDPI

Specialty Chemistry

Date 2022 Sep 23

PMID 36145348

Authors

Yulia Desheva

Igor Losev

Nadezhda Petkova

Polina Kudar

Svetlana Donina

Andrey Mamontov

Chih-Hsuan Tsai

Yu-Chan Chao

Affiliations

Soon will be listed here.

Abstract

Influenza outbreaks caused by A/H7N9 viruses have occurred since 2013. After 2016, A/H7N9 influenza viruses underwent evolutionary changes. In this study, we examined the antigenic properties of influenza neuraminidase (NA) of A/H7N9 viruses as part of a live influenza vaccine (LAIV). It was shown that neuraminidase inhibiting (NI) antibodies obtained after A/Anhui/1/2013(H7N9)-based LAIV vaccination did not inhibit A/Hong Kong/125/2017(H7N9) NA and vice versa. The A/Hong Kong/125/2017(H7N9)-based LAIV elicited higher levels of NI antibodies compared to the A/Anhui/1/2013(H7N9)-based LAIV after two doses. Thelow degree of coincidence of the antibody response to hemagglutinin (HA) and NA after LAIV vaccination allows us to consider an enzyme-linked lectin assay (ELLA) as an additional measure for assessing the immunogenicity of influenza vaccines. In mice, N9-reactive monoclonal antibodies (mABs) for the A/environment/Shanghai/RL01/2013(H7N9) influenza virus partially protected against lung infection from the A/Guangdong/17SF003/2016 IDCDC-RG56N(H7N9) virus, thus showing the cross-protective properties of monoclonal antibodies against the drift variant.

Citing Articles

Longitudinal Analysis of Neuraminidase and Hemagglutinin Antibodies to Influenza A Viruses after Immunization with Seasonal Inactivated Influenza Vaccines.

Sergeeva M, Romanovskaya-Romanko E, Krivitskaya V, Kudar P, Petkova N, Kudria K Vaccines (Basel). 2023; 11(11).

PMID: 38006063 PMC: 10675551. DOI: 10.3390/vaccines11111731.

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