Silver Nanoparticles Conjugated with Colistin Enhanced the Antimicrobial Activity Against Gram-Negative Bacteria

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Sep 23

PMID 36144516

Authors

Poowadon Muenraya

Somchai Sawatdee

Teerapol Srichana

Apichart Atipairin

Affiliations

Soon will be listed here.

Abstract

Colistin is a potent peptide antibiotic that is effective against Gram-negative bacteria. However, nephrotoxicity limited its clinical use. Silver nanoparticles (AgNPs) have gained attention as a potential antimicrobial agent and nanodrug carrier. The conjugation of antibiotics and AgNPs has been found to increase the activity and decrease drug toxicity. In this study, colistin was conjugated with AgNPs (Col-AgNPs), which was confirmed by Fourier-transform infrared (FT-IR) and energy-dispersive X-ray (EDX) spectra. The optimized Col-AgNPs had the proper characteristics, including spherical shape, monodispersity, nanosized particle, high surface charge, and good stability. The powder X-ray diffraction (PXRD) pattern supported the crystallinity of Col-AgNPs and AgNPs. The drug loading of Col-AgNPs was 11.55 ± 0.93%. Col-AgNPs had higher activity against Gram-negative bacteria (, , and ) than AgNPs and colistin. The mechanism of actions of Col-AgNPs involved membrane disruption and genomic DNA damage. The Col-AgNPs and AgNPs were biocompatible with human red blood cells and renal cells at concentrations up to 16 µg/mL. Interestingly, Col-AgNPs exhibited higher cell survival than AgNPs and colistin at 32 µg/mL. Our results revealed that the Col-AgNPs could enhance the antimicrobial activity and cell biocompatibility more than colistin and AgNPs.

Citing Articles

Colistin-niclosamide-loaded nanoemulsions and nanoemulsion gels for effective therapy of colistin-resistant infections.

Zhang J, Wang X, Li P, Gao Y, Wang R, Li S Front Vet Sci. 2024; 11:1492543.

PMID: 39507218 PMC: 11539104. DOI: 10.3389/fvets.2024.1492543.

Synthesis, Characterization, and Biological Evaluation of Chitosan Nanoparticles Cross-Linked with Phytic Acid and Loaded with Colistin against Extensively Drug-Resistant Bacteria.

Pacheco F, Barrera A, Ciro Y, Polo-Ceron D, Salamanca C, Onate-Garzon J Pharmaceutics. 2024; 16(9).

PMID: 39339153 PMC: 11435368. DOI: 10.3390/pharmaceutics16091115.

Antibiotic-Polyphosphate Nanocomplexes: A Promising System for Effective Biofilm Eradication.

To D, Blanco Massani M, Coraca-Huber D, Seybold A, Ricci F, Zoller K Int J Nanomedicine. 2024; 19:9707-9725.

PMID: 39309185 PMC: 11416784. DOI: 10.2147/IJN.S473241.

Excellent antibacterial and anti-inflammatory efficacy of amoxicillin by AgNPs and their conjugates synthesized using crude flavonoid extracts.

Jalil K, Ahmad S, Islam N, Muhammad S, Jalil Q, Ali A Heliyon. 2024; 10(17):e36752.

PMID: 39281441 PMC: 11399619. DOI: 10.1016/j.heliyon.2024.e36752.

Poly(2-Deoxy-2-Methacrylamido-D-Glucose)-Based Complex Conjugates of Colistin, Deferoxamine and Vitamin B12: Synthesis and Biological Evaluation.

Stepanova M, Levit M, Egorova T, Nashchekina Y, Sall T, Demyanova E Pharmaceutics. 2024; 16(8).

PMID: 39204425 PMC: 11359296. DOI: 10.3390/pharmaceutics16081080.

References

Miller S, Bell C, Mejias R, McClain M, Cover T, Giorgio T . Colistin-Functionalized Nanoparticles for the Rapid Capture of Acinetobacter baumannii. J Biomed Nanotechnol. 2018; 12(9):1806-19. DOI: 10.1166/jbn.2016.2273. View

Lee S, Kim J, Ravichandran K, Gil H, Song H, Hong S . P-Glycoprotein Induction Ameliorates Colistin Induced Nephrotoxicity in Cultured Human Proximal Tubular Cells. PLoS One. 2015; 10(8):e0136075. PMC: 4545883. DOI: 10.1371/journal.pone.0136075. View

Anastasiadis S, Chrissopoulou K, Stratakis E, Kavatzikidou P, Kaklamani G, Ranella A . How the Physicochemical Properties of Manufactured Nanomaterials Affect Their Performance in Dispersion and Their Applications in Biomedicine: A Review. Nanomaterials (Basel). 2022; 12(3). PMC: 8840392. DOI: 10.3390/nano12030552. View

Liu Y, Kuo S, Yao B, Fang Z, Lee Y, Chang Y . Colistin nanoparticle assembly by coacervate complexation with polyanionic peptides for treating drug-resistant gram-negative bacteria. Acta Biomater. 2018; 82:133-142. DOI: 10.1016/j.actbio.2018.10.013. View

Luna-Vazquez-Gomez R, Arellano-Garcia M, Toledano-Magana Y, Garcia-Ramos J, Radilla-Chavez P, Salas-Vargas D . Bell Shape Curves of Hemolysis Induced by Silver Nanoparticles: Review and Experimental Assay. Nanomaterials (Basel). 2022; 12(7). PMC: 9000491. DOI: 10.3390/nano12071066. View

Ordooei Javan A, Shokouhi S, Sahraei Z . A review on colistin nephrotoxicity. Eur J Clin Pharmacol. 2015; 71(7):801-10. DOI: 10.1007/s00228-015-1865-4. View

Goodman C, McCusker C, Yilmaz T, Rotello V . Toxicity of gold nanoparticles functionalized with cationic and anionic side chains. Bioconjug Chem. 2004; 15(4):897-900. DOI: 10.1021/bc049951i. View

Cheon J, Kim S, Rhee Y, Kwon O, Park W . Shape-dependent antimicrobial activities of silver nanoparticles. Int J Nanomedicine. 2019; 14:2773-2780. PMC: 6499446. DOI: 10.2147/IJN.S196472. View

De Lima R, Seabra A, Duran N . Silver nanoparticles: a brief review of cytotoxicity and genotoxicity of chemically and biogenically synthesized nanoparticles. J Appl Toxicol. 2012; 32(11):867-79. DOI: 10.1002/jat.2780. View

10.

Huang H, Lai W, Cui M, Liang L, Lin Y, Fang Q . An Evaluation of Blood Compatibility of Silver Nanoparticles. Sci Rep. 2016; 6:25518. PMC: 4857076. DOI: 10.1038/srep25518. View

11.

Akter M, Sikder M, Rahman M, Ullah A, Hossain K, Banik S . A systematic review on silver nanoparticles-induced cytotoxicity: Physicochemical properties and perspectives. J Adv Res. 2018; 9:1-16. PMC: 6057238. DOI: 10.1016/j.jare.2017.10.008. View

12.

Lim L, Ly N, Anderson D, Yang J, Macander L, Jarkowski 3rd A . Resurgence of colistin: a review of resistance, toxicity, pharmacodynamics, and dosing. Pharmacotherapy. 2010; 30(12):1279-91. PMC: 4410713. DOI: 10.1592/phco.30.12.1279. View

13.

Chen J, Ahmed M, Zhu C, Yu S, Pan W, Velkov T . In vitro evaluation of drug delivery behavior for inhalable amorphous nanoparticle formulations in a human lung epithelial cell model. Int J Pharm. 2021; 596:120211. PMC: 7904636. DOI: 10.1016/j.ijpharm.2021.120211. View

14.

Carlson C, Hussain S, Schrand A, Braydich-Stolle L, Hess K, Jones R . Unique cellular interaction of silver nanoparticles: size-dependent generation of reactive oxygen species. J Phys Chem B. 2008; 112(43):13608-19. DOI: 10.1021/jp712087m. View

15.

Evans B, Nelson C, Yu S, Beavers K, Kim A, Li H . Ex vivo red blood cell hemolysis assay for the evaluation of pH-responsive endosomolytic agents for cytosolic delivery of biomacromolecular drugs. J Vis Exp. 2013; (73):e50166. PMC: 3626231. DOI: 10.3791/50166. View

16.

Wang J, Fei K, Yang X, Zhang S, Peng Y . Synthesis and Plasmonic Chiroptical Studies of Sodium Deoxycholate Modified Silver Nanoparticles. Materials (Basel). 2018; 11(8). PMC: 6117651. DOI: 10.3390/ma11081291. View

17.

Masri A, Anwar A, Ahmed D, Siddiqui R, Raza Shah M, Khan N . Silver Nanoparticle Conjugation-Enhanced Antibacterial Efficacy of Clinically Approved Drugs Cephradine and Vildagliptin. Antibiotics (Basel). 2018; 7(4). PMC: 6316254. DOI: 10.3390/antibiotics7040100. View

18.

Khan T, Yasmin A, Townley H . An evaluation of the activity of biologically synthesized silver nanoparticles against bacteria, fungi and mammalian cell lines. Colloids Surf B Biointerfaces. 2020; 194:111156. DOI: 10.1016/j.colsurfb.2020.111156. View

19.

Piella J, Bastus N, Puntes V . Size-Dependent Protein-Nanoparticle Interactions in Citrate-Stabilized Gold Nanoparticles: The Emergence of the Protein Corona. Bioconjug Chem. 2016; 28(1):88-97. DOI: 10.1021/acs.bioconjchem.6b00575. View

20.

Kanwal Z, Raza M, Riaz S, Manzoor S, Tayyeb A, Sajid I . Synthesis and characterization of silver nanoparticle-decorated cobalt nanocomposites (Co@AgNPs) and their density-dependent antibacterial activity. R Soc Open Sci. 2019; 6(5):182135. PMC: 6549958. DOI: 10.1098/rsos.182135. View