Synbiotic Supplementation Modulates Gut Microbiota, Regulates β-Catenin Expression and Prevents Weight Gain in Ob/ob Mice: Preliminary Findings

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Sep 23

PMID 36142396

Authors

Sebastiao Mauro B Duarte

Jose Tadeu Stefano

Lucas A M Franco

Roberta C Martins

Bruna D G C Moraes

Denise Frediani Barbeiro

Nathalia Oliveira

Junia Marielle Teixeira Rodrigues Neri

Bruno Cogliati

Denise Siqueira Vanni

Ester C Sabino

Flair J Carrilho

Claudia P Oliveira

Affiliations

Soon will be listed here.

Abstract

Background: Obesity is one of the main health problems in the world today, and dysbiosis seems to be one of the factors involved. The aim of this study was to examine the impact of synbiotic supplementation on obesity and the microbiota in ob/ob mice. Twenty animals were divided into four groups: obese treated (OT), obese control (OC), lean treated (LT) and lean control (LC). All animals received a standard diet for 8 weeks. The treated groups received a synbiotic (Simbioflora-Invictus Farmanutrição Ltd., Sao Paulo, Brazil) in water, while the nontreated groups received only water. After 8 weeks, all animals were sacrificed, and gut tissue and stool samples were collected for mRNA isolation and microbiota analysis, respectively. β-Catenin, occludin, cadherin and zonulin in the gut tissue were analyzed via RT-qPCR. Microbiome DNA was extracted from stool samples and sequenced using an Ion PGM Torrent platform.

Results: Synbiotic supplementation reduced body weight gain in the OT group compared with the OC group ( = 0.0398) and was associated with an increase in Enterobacteriaceae ( = 0.005) and a decrease in Cyanobacteria ( = 0.047), ( = 0.026), ( = 0.005) and ( = 0.047). On the other hand, a significant reduction in ( = 0.009) and ( = 0.005) bacteria was observed in the LT group compared to the LC group. Alpha and beta diversities were different among all treated groups. β-Catenin gene expression was significantly decreased in the gut tissue of the OT group ( ≤ 0.0001) compared to the other groups. No changes were observed in occludin, cadherin or zonulin gene expression in the gut tissue.

Conclusions: Synbiotic supplementation prevents excessive weight gain, modulates the gut microbiota, and reduces β-catenin expression in ob/ob mice.

References

Monga S . β-Catenin Signaling and Roles in Liver Homeostasis, Injury, and Tumorigenesis. Gastroenterology. 2015; 148(7):1294-310. PMC: 4494085. DOI: 10.1053/j.gastro.2015.02.056. View

Ku C, Yang Y, Park Y, Lee J . Health benefits of blue-green algae: prevention of cardiovascular disease and nonalcoholic fatty liver disease. J Med Food. 2013; 16(2):103-11. PMC: 3576896. DOI: 10.1089/jmf.2012.2468. View

Vazquez-Baeza Y, Pirrung M, Gonzalez A, Knight R . EMPeror: a tool for visualizing high-throughput microbial community data. Gigascience. 2013; 2(1):16. PMC: 4076506. DOI: 10.1186/2047-217X-2-16. View

Caporaso J, Lauber C, Walters W, Berg-Lyons D, Lozupone C, Turnbaugh P . Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. Proc Natl Acad Sci U S A. 2010; 108 Suppl 1:4516-22. PMC: 3063599. DOI: 10.1073/pnas.1000080107. View

Auchtung T, Holder M, Gesell J, Ajami N, Duarte R, Itoh K . Complete Genome Sequence of Turicibacter sp. Strain H121, Isolated from the Feces of a Contaminated Germ-Free Mouse. Genome Announc. 2016; 4(2). PMC: 4807225. DOI: 10.1128/genomeA.00114-16. View

Ferrario C, Taverniti V, Milani C, Fiore W, Laureati M, De Noni I . Modulation of fecal Clostridiales bacteria and butyrate by probiotic intervention with Lactobacillus paracasei DG varies among healthy adults. J Nutr. 2014; 144(11):1787-96. DOI: 10.3945/jn.114.197723. View

Zhou Z, Chen J, Yao H, Hu H . and Colorectal Cancer. Front Oncol. 2018; 8:371. PMC: 6196248. DOI: 10.3389/fonc.2018.00371. View

Cotillard A, Kennedy S, Kong L, Prifti E, Pons N, Le Chatelier E . Dietary intervention impact on gut microbial gene richness. Nature. 2013; 500(7464):585-8. DOI: 10.1038/nature12480. View

Xie C, Halegoua-DeMarzio D . Role of Probiotics in Non-alcoholic Fatty Liver Disease: Does Gut Microbiota Matter?. Nutrients. 2019; 11(11). PMC: 6893593. DOI: 10.3390/nu11112837. View

10.

Schrezenmeir J, de Vrese M . Probiotics, prebiotics, and synbiotics--approaching a definition. Am J Clin Nutr. 2001; 73(2 Suppl):361S-364S. DOI: 10.1093/ajcn/73.2.361s. View

11.

Schinner S . Wnt-signalling and the metabolic syndrome. Horm Metab Res. 2009; 41(2):159-63. DOI: 10.1055/s-0028-1119408. View

12.

Tao J, Calvisi D, Ranganathan S, Cigliano A, Zhou L, Singh S . Activation of β-catenin and Yap1 in human hepatoblastoma and induction of hepatocarcinogenesis in mice. Gastroenterology. 2014; 147(3):690-701. PMC: 4143445. DOI: 10.1053/j.gastro.2014.05.004. View

13.

Yao F, Jia R, Huang H, Yu Y, Mei L, Bai L . Effect of N1115 and fructooligosaccharides in nonalcoholic fatty liver disease. Arch Med Sci. 2019; 15(5):1336-1344. PMC: 6764303. DOI: 10.5114/aoms.2019.86611. View

14.

Benzler J, Andrews Z, Pracht C, Stohr S, Shepherd P, Grattan D . Hypothalamic WNT signalling is impaired during obesity and reinstated by leptin treatment in male mice. Endocrinology. 2013; 154(12):4737-45. DOI: 10.1210/en.2013-1746. View

15.

Campbell H, Maiers J, DeMali K . Interplay between tight junctions & adherens junctions. Exp Cell Res. 2017; 358(1):39-44. PMC: 5544570. DOI: 10.1016/j.yexcr.2017.03.061. View

16.

Goretsky T, Bradford E, Ryu H, Tahir M, Moyer M, Gao T . A Cytosolic Multiprotein Complex Containing p85α Is Required for β-Catenin Activation in Colitis and Colitis-associated Cancer. J Biol Chem. 2015; 291(8):4166-77. PMC: 4759191. DOI: 10.1074/jbc.M115.669416. View

17.

Anstee Q, Goldin R . Mouse models in non-alcoholic fatty liver disease and steatohepatitis research. Int J Exp Pathol. 2006; 87(1):1-16. PMC: 2517349. DOI: 10.1111/j.0959-9673.2006.00465.x. View

18.

Horie M, Miura T, Hirakata S, Hosoyama A, Sugino S, Umeno A . Comparative analysis of the intestinal flora in type 2 diabetes and nondiabetic mice. Exp Anim. 2017; 66(4):405-416. PMC: 5682353. DOI: 10.1538/expanim.17-0021. View

19.

Ahmad A, Ali T, Kim M, Khan A, Jo M, Rehman S . Adiponectin homolog novel osmotin protects obesity/diabetes-induced NAFLD by upregulating AdipoRs/PPARα signaling in ob/ob and db/db transgenic mouse models. Metabolism. 2018; 90:31-43. DOI: 10.1016/j.metabol.2018.10.004. View

20.

Shinde T, Perera A, Vemuri R, Gondalia S, Beale D, Karpe A . Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases. Eur J Nutr. 2020; 59(8):3669-3689. PMC: 7669818. DOI: 10.1007/s00394-020-02200-9. View