An Immunological Polysaccharide from : Essential Role of Acetylation in Immunomodulation

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Sep 23

PMID 36142298

Authors

Tzu-Yin Huang

Feng-Ling Yang

Hsiao-Wen Chiu

Hong-Chu Chao

Yen-Ju Yang

Jyh-Horng Sheu

Kuo-Feng Hua

Shih-Hsiung Wu

Affiliations

Soon will be listed here.

Abstract

The edible fungus was shown to have a high molecular weight (1.87 × 10 kDa) bioactive polysaccharide, denoted as TFP-F1. Monosaccharide composition and NMR analysis of the polysaccharide and its derivatives indicated it contained fucose (Fuc), xylose (Xyl), mannose (Man), and glucuronic acid (GlcA) in a ratio of 0.9:1.0:3.2:1.2. Using IR, NMR, and GC-MS spectroscopic data, the structure of TFP-F1 was elucidated as {→3)-[-D-GlcA-(1→2)]--D-Man-(1→3)--D-Man-(1→3)-[-L-Fuc-(1→2)--D-Xyl-(1→2)]--D-Man-(1→}, with partial acetylation of C6-OH in mannoses. Furthermore, at a concentration of 1 μg/mL, TFP-F1 was found to stimulate the secretion of TNF-α and IL-6 in J774A.1 macrophage cells in vitro via interaction with toll-like receptor 4 (TLR4). The removal of -acetyl groups led to the loss of immunomodulatory activities, demonstrating that -acetyl groups play an essential role in enhancing the production of pro-inflammatory cytokines.

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