Trace Immunosensing of Multiple Neonicotinoid Insecticides by a Novel Broad-Specific Antibody Obtained from a Rational Screening Strategy

Overview

Journal Biosensors (Basel)

Specialty Biotechnology

Date 2022 Sep 23

PMID 36140100

Authors

Shasha Jiao

Yan Wang

Yunyun Chang

Pengyan Liu

Yang Chen

Yihua Liu

Guonian Zhu

Yirong Guo

Affiliations

Soon will be listed here.

Abstract

Residues of neonicotinoid pesticides have potential risks to food, environmental and biological safety. In this study, the hapten toward imidacloprid was adopted to gain antibodies. After molecular modeling, the electrostatic potentials of eight commonly-used neonicotinoid pesticides were individually calculated to analyze the structural similarity. Two representative compounds (imidacloprid and acetamiprid) with moderate similarity were rationally selected for hybridoma screening. Using this strategy, four clones of broad-specific monoclonal antibodies (mAbs) against multiple neonicotinoids were obtained, and the clone 6F11 exhibited the broadest spectrum to six neonicotinoid pesticides and two metabolites, with half-maximal inhibitory concentrations (IC) ranging from 0.20 to 5.92 ng/mL. Then, the novel antibody gene was sequenced and successfully expressed in full-length IgG form using mammalian cells. Based on the sensitive recombinant antibody, a gold lateral-flow immunosensing strip assay was developed and it was qualified for rapid detection of imidacloprid, clothianidin or imidaclothiz residues in food samples.

Citing Articles

Development of colloidal carbon-based immunoassay for the determination of thiamethoxam in medicinal and edible Lonicerae Japonicae Flos.

Guo M, Ke T, Zhang H, Zhang J, Qin J, Ruan H Anal Bioanal Chem. 2025; 417(8):1595-1606.

PMID: 39890623 DOI: 10.1007/s00216-025-05749-8.

Development and Validation of a High-Performance Liquid Chromatography Diode Array Detector Method to Measure Seven Neonicotinoids in Wheat.

Seccia S, Albrizio S, Morelli E, Dini I Foods. 2024; 13(14).

PMID: 39063319 PMC: 11275328. DOI: 10.3390/foods13142235.

On-site rapid detection of multiple pesticide residues in tea leaves by lateral flow immunoassay.

Gao J, Zhang T, Fang Y, Zhao Y, Yang M, Zhao L J Pharm Anal. 2024; 14(2):276-283.

PMID: 38464789 PMC: 10921326. DOI: 10.1016/j.jpha.2023.09.011.

References

Fang S, Zhang B, Ren K, Cao M, Shi H, Wang M . Development of a sensitive Indirect Competitive Enzyme-Linked Immunosorbent Assay (ic-ELISA) based on the monoclonal antibody for the detection of the imidaclothiz residue. J Agric Food Chem. 2011; 59(5):1594-7. DOI: 10.1021/jf104241n. View

Liu P, Guo Y, Jiao S, Chang Y, Liu Y, Zou R . Characterization of Variable Region Genes and Discovery of Key Recognition Sites in the Complementarity Determining Regions of the Anti-Thiacloprid Monoclonal Antibody. Int J Mol Sci. 2020; 21(18). PMC: 7555632. DOI: 10.3390/ijms21186857. View

Hallmann C, Foppen R, van Turnhout C, de Kroon H, Jongejans E . Declines in insectivorous birds are associated with high neonicotinoid concentrations. Nature. 2014; 511(7509):341-3. DOI: 10.1038/nature13531. View

Humphrey W, Dalke A, Schulten K . VMD: visual molecular dynamics. J Mol Graph. 1996; 14(1):33-8, 27-8. DOI: 10.1016/0263-7855(96)00018-5. View

Hladik M, Main A, Goulson D . Environmental Risks and Challenges Associated with Neonicotinoid Insecticides. Environ Sci Technol. 2018; 52(6):3329-3335. DOI: 10.1021/acs.est.7b06388. View

Li H, Ma S, Zhang X, Li C, Dong B, Mujtaba M . Generic Hapten Synthesis, Broad-Specificity Monoclonal Antibodies Preparation, and Ultrasensitive ELISA for Five Antibacterial Synergists in Chicken and Milk. J Agric Food Chem. 2018; 66(42):11170-11179. DOI: 10.1021/acs.jafc.8b03834. View

Xie H, Li Y, Wang J, Lei Y, Koidis A, Li X . Broad-specific immunochromatography for simultaneous detection of various sulfonylureas in adulterated multi-herbal tea. Food Chem. 2021; 370:131055. DOI: 10.1016/j.foodchem.2021.131055. View

Lu T, Chen F . Multiwfn: a multifunctional wavefunction analyzer. J Comput Chem. 2011; 33(5):580-92. DOI: 10.1002/jcc.22885. View

Wang J, Wang Y, Pan Y, Chen D, Liu Z, Feng L . Preparation of a generic monoclonal antibody and development of a highly sensitive indirect competitive ELISA for the detection of phenothiazines in animal feed. Food Chem. 2016; 221:1004-1013. DOI: 10.1016/j.foodchem.2016.11.062. View

10.

Watanabe E, Miyake S, Yogo Y . Review of enzyme-linked immunosorbent assays (ELISAs) for analyses of neonicotinoid insecticides in agro-environments. J Agric Food Chem. 2013; 61(51):12459-72. DOI: 10.1021/jf403801h. View

11.

Yu X, Zhang X, Xu J, Guo P, Li X, Wang H . Generation of recombinant antibodies by mammalian expression system for detecting S-metolachlor in environmental waters. J Hazard Mater. 2021; 418:126305. DOI: 10.1016/j.jhazmat.2021.126305. View

12.

Liang J, Liu Z, Xie H, Fang Y, Quan Q, Shen X . Ultrasensitive Magnetic Assisted Lateral Flow Immunoassay Based on Chiral Monoclonal Antibody against -(-)-Salbutamol of Broad-Specificity for 38 β-Agonists Detection in Swine Urine and Pork. J Agric Food Chem. 2022; 70(13):4112-4122. DOI: 10.1021/acs.jafc.2c00875. View

13.

Wang P, Yang X, Wang J, Cui J, Dong A, Zhao H . Multi-residue method for determination of seven neonicotinoid insecticides in grains using dispersive solid-phase extraction and dispersive liquid-liquid micro-extraction by high performance liquid chromatography. Food Chem. 2014; 134(3):1691-8. DOI: 10.1016/j.foodchem.2012.03.103. View

14.

Zhang C, Yi X, Chen C, Tian D, Liu H, Xie L . Contamination of neonicotinoid insecticides in soil-water-sediment systems of the urban and rural areas in a rapidly developing region: Guangzhou, South China. Environ Int. 2020; 139:105719. DOI: 10.1016/j.envint.2020.105719. View

15.

Zou R, Guo Y, Chen Y, Zhao Y, Zhao L, Zhu G . Computer-aided profiling of a unique broad-specific antibody and its application to an ultrasensitive fluoroimmunoassay for five N-methyl carbamate pesticides. J Hazard Mater. 2021; 426:127845. DOI: 10.1016/j.jhazmat.2021.127845. View

16.

Yang B, Ma W, Wang S, Shi L, Li X, Ma Z . Determination of eight neonicotinoid insecticides in Chinese cabbage using a modified QuEChERS method combined with ultra performance liquid chromatography-tandem mass spectrometry. Food Chem. 2022; 387:132935. DOI: 10.1016/j.foodchem.2022.132935. View

17.

Chang Y, Chen Y, Jiao S, Lu X, Fang Y, Liu Y . A Novel Full-length IgG Recombinant Antibody Highly Specific to Clothianidin and Its Application in Immunochromatographic Assay. Biosensors (Basel). 2022; 12(4). PMC: 9032790. DOI: 10.3390/bios12040233. View

18.

Tan G, Zhao Y, Wang M, Chen X, Wang B, Li Q . Ultrasensitive quantitation of imidacloprid in vegetables by colloidal gold and time-resolved fluorescent nanobead traced lateral flow immunoassays. Food Chem. 2019; 311:126055. DOI: 10.1016/j.foodchem.2019.126055. View

19.

Cao L, Kong D, Sui J, Jiang T, Li Z, Ma L . Broad-specific antibodies for a generic immunoassay of quinolone: development of a molecular model for selection of haptens based on molecular field-overlapping. Anal Chem. 2009; 81(9):3246-51. DOI: 10.1021/ac802403a. View

20.

Hao C, Noestheden M, Zhao X, Morse D . Liquid chromatography-tandem mass spectrometry analysis of neonicotinoid pesticides and 6-chloronicotinic acid in environmental water with direct aqueous injection. Anal Chim Acta. 2016; 925:43-50. DOI: 10.1016/j.aca.2016.04.024. View