Novel Megaplasmid Driving NDM-1-Mediated Carbapenem Resistance in ST1588 in South America

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2022 Sep 23

PMID 36139987

Authors

Mario Quezada-Aguiluz

Andres Opazo-Capurro

Nilton Lincopan

Fernanda Esposito

Bruna Fuga

Sergio Mella-Montecino

Gisela Riedel

Celia A Lima

Helia Bello-Toledo

Marcela Cifuentes

Francisco Silva-Ojeda

Boris Barrera

Juan C Hormazabal

Gerardo Gonzalez-Rocha

Affiliations

Soon will be listed here.

Abstract

Carbapenem-resistant Enterobacterales (CRE) is a critical public health problem in South America, where the prevalence of NDM metallo-betalactamases has increased substantially in recent years. In this study, we used whole genome sequencing to characterize a multidrug-resistant (MDR) (UCO-361 strain) clinical isolate from a teaching hospital in Chile. Using long-read (Nanopore) and short-read (Illumina) sequence data, we identified a novel un-typeable megaplasmid (314,976 kb, pNDM-1_UCO-361) carrying the carbapenem resistance gene within a Tn transposon. Strikingly, conjugal transfer of pNDM-1_UCO-361 plasmid only occurs at low temperatures with a high frequency of 4.3 × 10 transconjugants/receptors at 27 °C. UCO-361 belonged to the ST1588 clone, previously identified in Latin America, and harbored aminoglycoside, extended-spectrum β-lactamases (ESBLs), carbapenem, and quinolone-resistance determinants. These findings suggest that -bearing megaplasmids can be adapted to carriage by some lineages, whereas its conjugation at low temperatures could contribute to rapid dissemination at the human-environmental interface.

Citing Articles

Genomic Diversity of NDM-Producing Species from Brazil, 2013-2022.

Camargo C, Yamada A, Souza A, Reis A, Santos M, Assis D Antibiotics (Basel). 2022; 11(10).

PMID: 36290053 PMC: 9598336. DOI: 10.3390/antibiotics11101395.

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