Stress-Induced Immunosuppression Affects Immune Response to Newcastle Disease Virus Vaccine Via Circulating MiRNAs

Overview

Journal Animals (Basel)

Date 2022 Sep 23

PMID 36139236

Authors

Yufei Tian

Yang Liu

Qiuyuan Wang

Jie Wen

Yiru Wu

Jianwei Han

Chaolai Man

Affiliations

Soon will be listed here.

Abstract

Studies have shown that circulating microRNAs (miRNAs) are important players in the immune response and stress-induced immunosuppression. However, the function and mechanism of stress-induced immunosuppression affecting the immune response to the Newcastle disease virus (NDV) vaccine remain largely unknown. This study analyzed the changes of 15 NDV-related circulating miRNAs at different immune stages by qRT-PCR, aiming to explore the key timepoints, potential biomarkers, and mechanisms for the functional regulation of candidate circulating miRNAs under immunosuppressed conditions. The results showed that stress-induced immunosuppression induced differential expressions of the candidate circulating miRNAs, especially at 2 days post immunization (dpi), 14 dpi, and 28 dpi. In addition, stress-induced immunosuppression significantly affected the immune response to NDV vaccine, which was manifested by significant changes in candidate circulating miRNAs at 2 dpi, 5 dpi, and 21 dpi. The featured expressions of candidate circulating miRNAs indicated their potential application as biomarkers in immunity and immunosuppression. Bioinformatics analysis revealed that the candidate circulating miRNAs possibly regulated immune function through key targeted genes, such as Mg/Mn-dependent 1A () and Nemo-like kinase (), in the MAPK signaling pathway. This study provides a theoretical reference for studying the function and mechanism of circulating miRNAs in immune regulation.

References

Wang X, Jia Y, Ren J, Liu H, Xiao S, Wang X . MicroRNA gga-miR-455-5p suppresses Newcastle disease virus replication via targeting cellular suppressors of cytokine signaling 3. Vet Microbiol. 2019; 239:108460. DOI: 10.1016/j.vetmic.2019.108460. View

Fu X, Qie J, Fu Q, Chen J, Jin Y, Ding Z . miR-20a-5p/TGFBR2 Axis Affects Pro-inflammatory Macrophages and Aggravates Liver Fibrosis. Front Oncol. 2020; 10:107. PMC: 7031347. DOI: 10.3389/fonc.2020.00107. View

Schilling S, Datto M, Wang X . A phosphatase controls the fate of receptor-regulated Smads. Cell. 2006; 125(5):838-40. DOI: 10.1016/j.cell.2006.05.015. View

Nie F, Zhang J, Li M, Chang X, Duan H, Li H . Transcriptome analysis of thymic tissues from Chinese Partridge Shank chickens with or without Newcastle disease virus LaSota vaccine injection via high-throughput RNA sequencing. Bioengineered. 2022; 13(4):9131-9144. PMC: 9161911. DOI: 10.1080/21655979.2021.2008737. View

El-Kassas S, Abdo S, El-Naggar K, Abdo W, Kirrella A, Nashar T . Ameliorative effect of dietary supplementation of copper oxide nanoparticles on inflammatory and immune reponses in commercial broiler under normal and heat-stress housing conditions. J Therm Biol. 2018; 78:235-246. DOI: 10.1016/j.jtherbio.2018.10.009. View

Min S, Liang X, Zhang M, Zhang Y, Mei S, Liu J . Multiple tumor-associated microRNAs modulate the survival and longevity of dendritic cells by targeting YWHAZ and Bcl2 signaling pathways. J Immunol. 2013; 190(5):2437-46. DOI: 10.4049/jimmunol.1202282. View

Liu W, Kang S, Huang Z, Wu C, Jin H, Maine C . A miR-155-Peli1-c-Rel pathway controls the generation and function of T follicular helper cells. J Exp Med. 2016; 213(9):1901-19. PMC: 4995083. DOI: 10.1084/jem.20160204. View

Lu W, You R, Yuan X, Yang T, Samuel E, Marcano D . The microRNA miR-22 inhibits the histone deacetylase HDAC4 to promote T(H)17 cell-dependent emphysema. Nat Immunol. 2015; 16(11):1185-94. PMC: 4597310. DOI: 10.1038/ni.3292. View

Zhang G, Liu X, Wang W, Cai Y, Li S, Chen Q . Down-regulation of miR-20a-5p triggers cell apoptosis to facilitate mycobacterial clearance through targeting JNK2 in human macrophages. Cell Cycle. 2016; 15(18):2527-38. PMC: 5026815. DOI: 10.1080/15384101.2016.1215386. View

10.

Tian H, Ding M, Guo Y, Su A, Zhai M, Tian Y . Use of transcriptomic analysis to identify microRNAs related to the effect of stress on thymus immune function in a chicken stress model. Res Vet Sci. 2021; 140:233-241. DOI: 10.1016/j.rvsc.2021.09.004. View

11.

Wu X, Ye Y, Niu J, Li Y, Li X, You X . Defective PTEN regulation contributes to B cell hyperresponsiveness in systemic lupus erythematosus. Sci Transl Med. 2014; 6(246):246ra99. DOI: 10.1126/scitranslmed.3009131. View

12.

Li J, Che L, Xu C, Lu D, Xu Y, Liu M . XIST/miR-34a-5p/PDL1 axis regulated the development of lung cancer cells and the immune function of CD8 T cells. J Recept Signal Transduct Res. 2022; 42(5):469-478. DOI: 10.1080/10799893.2021.2019274. View

13.

Matur E, Akyazi I, Eraslan E, Ergul Ekiz E, Eseceli H, Keten M . The effects of environmental enrichment and transport stress on the weights of lymphoid organs, cell-mediated immune response, heterophil functions and antibody production in laying hens. Anim Sci J. 2015; 87(2):284-92. DOI: 10.1111/asj.12411. View

14.

Sapolsky R, Romero L, Munck A . How do glucocorticoids influence stress responses? Integrating permissive, suppressive, stimulatory, and preparative actions. Endocr Rev. 2000; 21(1):55-89. DOI: 10.1210/edrv.21.1.0389. View

15.

Kaufmann L, Syedbasha M, Vogt D, Hollenstein Y, Hartmann J, Linnik J . An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers. J Vis Exp. 2017; (130). PMC: 5755515. DOI: 10.3791/55833. View

16.

Yoshikawa Y, Hirata N, Terada H, Sawashita Y, Yamakage M . Identification of Candidate Genes and Pathways in Dexmedetomidine-Induced Cardioprotection in the Rat Heart by Bioinformatics Analysis. Int J Mol Sci. 2019; 20(7). PMC: 6480577. DOI: 10.3390/ijms20071614. View

17.

Liang X, Liu Y, Mei S, Zhang M, Xin J, Zhang Y . MicroRNA-22 impairs anti-tumor ability of dendritic cells by targeting p38. PLoS One. 2015; 10(3):e0121510. PMC: 4380340. DOI: 10.1371/journal.pone.0121510. View

18.

Lv Z, Li C, Zhang W, Jin C, Shao Y, Xuemei D . Nemo like kinase negatively regulates NF-κB activation and coelomocytes apoptosis in Apostichopus japonicus. Dev Comp Immunol. 2015; 54(1):109-15. DOI: 10.1016/j.dci.2015.09.002. View

19.

Daams R, Massoumi R . Nemo-Like Kinase in Development and Diseases: Insights from Mouse Studies. Int J Mol Sci. 2020; 21(23). PMC: 7731171. DOI: 10.3390/ijms21239203. View

20.

Cecchini S, Rossetti M, Tomaso F, Caputo A . Evaluation of the effects of dexamethasone-induced stress on levels of natural antibodies in immunized laying hens. Vet Immunol Immunopathol. 2016; 177:35-41. DOI: 10.1016/j.vetimm.2016.06.002. View