Analysis of Polyadenylation Signal Usage with Full-Length Transcriptome in (Lepidoptera: Noctuidae)

Overview

Journal Insects

Specialty Biology

Date 2022 Sep 22

PMID 36135504

Authors

Liying Fang

Lina Guo

Min Zhang

Xianchun Li

Zhongyuan Deng

Affiliations

Soon will be listed here.

Abstract

During the messenger RNA (mRNA) maturation process, RNA polyadenylation is a key step, and is coupled to the termination of transcription. Various cis-acting elements near the cleavage site and their binding factors would affect the process of polyadenylation, and AAUAAA, a highly conserved hexamer, was the most important polyadenylation signal (PAS). PAS usage is one of the critical modification determinants targeted at mRNA post-transcription. The full-length transcriptome has recently generated a massive amount of sequencing data, revealing poly(A) variation and alternative polyadenylation (APA) in . We identified 50,616 polyadenylation signals in via analysis of full-length transcriptome combined with expression Sequence Tags Technology (EST). The polyadenylation signal usage in is conserved, and it is similar to that of flies and other animals. AAUAAA and AUUAAA are the most highly conserved polyadenylation signals of all polyadenylation signals we identified. Additionally, we found the U/GU-rich downstream sequence element (DSE) in the cleavage site. These results demonstrate that APA in plays a significant role in root growth and development. This is the first polyadenylation signal usage analysis in agricultural pests, which can deepen our understanding of and provide a theoretical basis for pest control.

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