Identification and Validation of Reference Genes for Expression Analysis Using QRT-PCR in (Hemiptera: Cimicidae)

Overview

Journal Insects

Specialty Biology

Date 2022 Sep 22

PMID 36135485

Authors

Delong Kong

Daxia Shi

Changlu Wang

Ruyue Zhai

Lingling Lyu

Yurong He

Desen Wang

Affiliations

Soon will be listed here.

Abstract

The tropical bed bug, (F.) (Hemiptera: Cimicidae) is an important public-health pest that feeds on the blood of humans and some other animals. To explore the function of the target genes of , it is essential to select suitable reference genes for the accurate quantification of gene expression. Here, we selected 10 frequently used reference genes in insects and evaluated their stability in under various biotic (developmental stage, sex, and tissue) and abiotic (gas stimulation and temperature) conditions through RefFinder (which integrates four computational programs: geNorm, NormFinder, BestKeeper, and ∆Ct). Results indicate that the optimal combination of reference genes for each experimental condition was as follows: and for the developmental stage (eggs, early instar nymphs, late instar nymphs, and adults), and for adult sex, and for adult tissue (head, thorax, abdomen, and legs), and for gas stimulation (air and carbon dioxide), and and for temperature (0, 5, 17, 30, and 37 °C). Finally, the expression pattern of the and genes were analyzed, and the results highlight the importance of appropriate reference-gene selection. Our results provide a comprehensive list of optimal reference genes from for the first time, which will contribute to accurately analyzing the expression of target genes.

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