A Nano-biomimetic Transformation System Enables Expression of a Reporter Gene in Mature Plants and Seeds

Overview

Journal Nanoscale Adv

Publisher Royal Society of Chemistry

Specialty Biotechnology

Date 2022 Sep 22

PMID 36133668

Authors

Chinenye L Izuegbunam

Nisitha Wijewantha

Beate Wone

Madhavi A Ariyarathne

Grigoriy Sereda

Bernard W M Wone

Affiliations

Soon will be listed here.

Abstract

Plant genetic engineering will be essential to decipher the genomic basis of complex traits, optimize crop genomics, and enable plant-based production of recombinant proteins. However, established plant transformation approaches for bioengineering are fraught with limitations. Although nanoparticle-mediated methods show great promise for advancing plant biotechnology, many engineered nanomaterials can have cytotoxic and ecological effects. Here, we demonstrate the efficient uptake of a nano-biomimetic carrier of plasmid DNA and transient expression of a reporter gene in leaves of , common ice plant and tobacco, as well as in the developing seed tissues of , field mustard, barley, and wheat. The nano-biomimetic transformation system described here has all the advantages of other nanoparticle-mediated approaches for passive delivery of genetic cargo into a variety of plant species and is also nontoxic to cells and to the environment for diverse biotechnological applications in plant biology and crop science.

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